Callus induction and plant regeneration from cotyledonary explants of ash gourd (Benincasa hispida L.)

A protocol for the production of complete plantlets through multiple shoots from the cotyledon-derived calli of ash gourd (Benincasa hispida L.) is described. The embryos were excised from mature seeds and cultured on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurin (BAP, 1–5 μM). After 10 days the well-developed green cotyledons from the growing embryos were isolated and cultured on MS medium fortified with 2,4-D (1–6 μM). The cultured cotyledons gave rise to luxuriantly growing calli after 6 weeks. These calli were subcultured on MS medium supplemented with various concentrations of BAP (1–6 μM) alone or in combination with naphthalene acetic acid (NAA, 0.2 and 0.5 μM) for regeneration. The regenerated shoots were multiplied and rooted on quarter strength MS medium supplemented with indole-3-butyric acid or NAA (1–5 μM). The rooted shoots were transplanted to soil with 90% success.     

STUDY ON THE NATURAL REGENERATION EFFECT OF MANCHURIAN ASH BY OPENING UP EFFECT BELT IN DAHURIAN LARCH PLANTATION

NaturalregenerationofmanchurianashusuallytakesplacewheretherearesuitablesoilmoistureandseedbankofsecondarymanchurianashforestintheEasternMountainousAreaofNortheastChina.Sometimes,naturalregenerationofmanchurianashcanbefoundinpureconiferousplantation.Inordertofindoutthepossibilityofintroducingnaturalregenerationofmanchurianashinpureconiferousplantation,trialsandresear-cheshavebeencarriedoutindahurianlarchp1antationbyopeningupeffectbeltinthestands.lntheend,charactersofcommunitystructureandmicr…     

虉草热值与灰分动态变化研究

通过对虉草的热值和灰分进行测定,探讨虉草热值和灰分的动态变化及二者之间相关性.结果表明,虉草干重热值呈现双峰的变化趋势,峰值分别出现在孕穗期和成熟期;虉草灰分含量随物候期呈现先降低后升高的变化趋势,成熟期灰分含量最低;去灰分热值随着生育时期推迟呈现逐渐降低的变化趋势,其顺序为孕穗期＞抽穗期＞盛花期＞成熟期＞果后营养期＞枯黄期;各生育期干重热值与灰分含量之间均表现为负相关关系,其中抽穗期表现为极显著线性负相关(P＜0.01),孕穗期和果后营养期表现为显著线性负相关(P＜0.05),盛花期、成熟期和枯黄期相关性不显著.     

Tree identity surpasses tree diversity in affecting the community structure of oribatid mites (Oribatida) of deciduous temperate forests

The role of tree diversity and identity as determinants of soil animal community structure is little understood. In a mature deciduous forest dominated by beech we identified clusters of one, two and three tree species of beech, ash and lime allowing to investigate the role of tree species diversity and identity on the density and community structure of oribatid mites. To relate oribatid mite community structure to environmental factors we measured leaf litter input, fine root biomass, mass of organic layers, topsoil pH and C and N content. We expected oribatid mite density to increase with increasing tree diversity, but we expected the effects of tree species identity to override effects of tree diversity. Further, we hypothesized the density of oribatid mites to be reduced by the presence of beech but increased by the presence of lime and ash. As expected tree diversity little affected oribatid mite communities, whereas tree species identity strongly altered density and community structure of oribatid mites. However, in contrast to our expectations the density of oribatid mites was highest in presence of beech indicating that many oribatid mite species benefit from the presence of recalcitrant litter forming thick organic layers. Especially Oppioidea benefited from the presence of beech presumably due to an increased availability of food resources such as fungi and nematodes. Lower density of oribatid mites in monospecific clusters of lime and ash suggests that oribatid mites did not benefit from high quality litter of these species. Notably, large and strongly sclerotized oribatid mite species, such as Steganacarus magnus and Chamobates voigtsi, benefited from the presence of ash and lime. Presumably, these large species better resist harsh microclimatic conditions in shallow organic layers.     

THE CHARACTERS AND EFFECTS OF AVAILABLE PHOSPHORUS IN DAHURIAN LARCH-MANCHURICA ASH MIXED SEEDLING STAND

Dahurianlarch(Larixgmelinii)isamaintreesPeciesinartificialstandsandManchuriaash(Fnainusma:dehurica)isavaluabIehardwoodsPeciesinti1eforestareaofnortheastChina.SomestUdiesrevealedthatthemixedstandbythetwospecieshadpositivemixedeffectSandnutritionrelationshipwasoneofthemaincausesforthepositiveeffects.lnsomeresearches,itwasfoundthatinline-belttwoflarch-ashmixedstand,theconcentra-tionofavailablephosphorusofsoilinlarchbeltwas2ntimesoftl1atinashbelt.Itisdeducedthatinsuchmixedstandtheashcanabsorbmo…     

落叶松纯林与水曲柳落叶松混交林土壤理化性质分析

为了探讨水曲柳落叶松混交林的增产机理。文章从土壤理化性质，营养元素含量等方面，对松南县孟家岗林场２２年生落叶松纯林和水落混交林进行了调查分析。结果表明：混交林在较大幅度上改善了土壤的物理性质。混交林下土壤表层和底层容重分别比纯林降低１２．３８％和１８．７４％，总孔隙度提高１４．５１％和７．０１％；渗透速度分别比纯林提高６４．６０％和５２．１７％。混交林虽未能提高土壤ＰＨ值和全量元素含量，但表层土壤     

Biological soil amelioration as the basis of sustainable agriculture and forestry

Before the discovery of plant nutrients, soil amelioration and fertilization was by force of circumstance biological, i.e. done with substances taken from nature. After the discovery of plant nutrients, the chemical concept of fertility displaced the biological one. The defects of chemical cultivation — inefficiency, deterioration in product quality, diminishing productivity of soils and negative effects on the environment — have created an urgent need for the study of fertility as a result of the activity of the biocycles of the ecosystem. With the aid of the advances of modern science, we can understand the defects and deficiencies of the chemical concept of fertility and return the biological concept of fertility to its proper place. This in turn is a good starting point for productive research and developmental work towards an ecologically sustainable agriculture and forestry.     

微波马弗炉测定烟草中灰分的研究

[目的]建立烟草及其制品中灰分的分析方法。[方法]准确称取1.0 g烟末样品置于石英纤维坩埚中,于微波马弗炉中500℃灼烧20 min,取出置于干燥器中冷却至室温后准确称重,计算。[结果]与传统的马弗炉方法相比,灼烧时间由60 min缩短至20 min,相对标准偏差(RSD)在1.01%～1.58%,测定结果与传统方法吻合。因仪器本身有自动排空系统,灰化过程中产生的气体不断排放,可保证实验室的环境安全。[结论]为烟草及其制品灰分的测定提供了一种简便、快捷的方法。     

Blackening of sugi (Cryptomeria japonica D. Don) heartwood in relation to metal content and moisture content

Blackening in heartwood was investigated in relation to the metal contents and the moisture content in xylem of about 50-year-old seedling sugi (Cryptomeria japonica D. Don) planted in a steeply sloped stand in Okutama district (Itsukaichi Tokyo), where blackened heartwood is frequently found. The potassium, calcium, iron, and manganese contents were examined in the variously blackened heartwood and normal heartwood by an atomic absorption method. It was recognized that potassium increased relative to the degree of the blackening of heartwood, resulting in a significant correlation between them. This finding implies that an increase in potassium has an important role in the blackening of heartwood. Moisture content has a tendency to increase in the blackened heartwood, so it seems that the large accumulation of potassium is associated with the high moisture content in heartwood.This work was presented at the 43rd Annual Meeting of the Japan Wood Research Society at Morioka, August 1993     

Can carbon isotope discrimination and ash content predict grain yield and water use efficiency in wheat?

Drought is the main factor affecting crop grain yield. Increasing grain yield under drought and crop water use efficiency (WUE) is essential for enhancing world crop production and food availability. The objective of this study, carried out in India on 20 durum wheat cultivars, under three water regimes (full irrigation, limited irrigation and residual soil moisture) and during two seasons, was to investigate the potential use of plant traits (particularly carbon isotope discrimination, Δ, and ash content, m_a) to predict grain yield and WUE in wheat. WUE components were estimated using a soil water balance model (Budget) allowing comparison of environments in data scarce situations. A highly significant correlation was noted between grain yield and grain Δ across water regimes. However, the associations between grain yield, Δ and m_a were found to depend highly on the water regime and environmental conditions. The association between grain yield and grain Δ was significant under full irrigation in season 1 and under residual soil moisture in season 2. Significant positive correlations were noted in both seasons between grain yield and leaf Δ under residual soil moisture and between grain yield and leaf ash content at anthesis under limited irrigation. A significant correlation was found across environments between grain and leaf Δ and T, the quantity of water transpired during the growth cycle, as estimated by the soil water balance model. T also significantly correlated to grain and leaf m_a. Variation in WUE across environments was driven more by runoff, drainage and soil evaporation than by harvest index and transpiration. The associations between WUE and transpiration, runoff and Δ were negative but not significant. WUE was significantly correlated with leaf and grain m_a at maturity. The study indicates that Δ and m_a can be used as indirect selection criteria for grain yield and suggests that m_a is a good predictor of transpiration, grain yield and WUE across environments. The use of mechanistic models that allows differentiating between cultivars should permit in a next future to analyze the relationships between WUE, Δ and m_a across cultivars and evaluate the possibility to use these traits as predictors of WUE in wheat breeding programs.