Preliminary Study on NF-κB Signaling Pathway Regulating Brucella Intracellular Survival Molecular Mechanisms

By the infection of Brucella virulent strain and attenuated strain in mice macrophage RAW264.7,the assay was aimed to explore the relationship between NF-κB signaling pathways and Brucella virulent strain and attenuated strain in intracellular survival.Use different MOI Brucella (2308,RB51,16M and M5) to infect mice macrophage RAW264.7,after 0,4,8 and 24 h infected,cracking cell and collecting supernatant,we detected the effect of Brucella on activation of NF-κB signaling pathway by Western blotting.Different concentrations of NF-κB signaling pathway inhibitor were incubated with mice macrophage RAW264.7,with different multiplicities of infection (MOI) of Brucella infecting cells,ELISA kits to detect the expressions of TNF-α,IL-1β and IL-6 cytokine;At the same time,count the number of intracellular bacteria of CFU.The results showed that rough cattle Brucella strains RB51 could strongly activate NF-κB signaling pathway,smooth cattle Brucella strains 2308 was weak in the activation;At the same time,the activation of NF-κB signaling pathway was concentration dependent.When the MOI was 80,infection time was 8 h,NF-κB activation degrees of rough cattle Brucella strains RB51 and smooth cattle Brucella strains 2308 were the strongest,and this pathway was involved in producing TNF-α and IL-6;NF-κB signaling pathway inhibitor BAY11-7082 affected Brucella intracellular survival.So rough cattle Brucella strains RB51 intracellular survival and NF-κB signaling pathway activity were closely related.The results laid the foundation for the further study of Brucella intracellular pathogenesis,also provided scientific basis for the research of new drugs to Brucella,and prevention and treatment of brucellosis.     

水位检测远程传输系统

本系统以STC89C51单片机为控制核心,HC-SR04超声波模块进行测距,GSM模块进行远程传输[1],LCD12864和矩阵键盘进行数据显示和更改等操作。据实验,该系统测量结果准确、灵敏度高、使用可靠、性价比高,具有很好的实用价值。     

Identification of the core motif of the BRCA2 C-terminal RAD51-binding domain by comparing canine and human BRCA2

Mammary tumors are the most common tumors in women and non-spayed female dogs. One of the reasons for mammary tumors is mutations of the tumor suppressor gene, BRCA2. BRCA2 participates in homologous recombination repair by interacting with the RAD51 recombinase. BRCA2 has two RAD51-binding domains, consisting of BRC repeats and the C-terminal RAD51-binding domain, respectively. Although several studies have addressed the function of the C-terminal RAD51-binding domain of human BRCA2, the amino acid sequences required for the RAD51-interaction activity remain unclear. In this study, the C-terminal RAD51-binding domains of canine and human BRCA2 were compared; the canine domain displayed a weaker interaction with RAD51. This difference was attributed to the C-terminal portion of the domain via a comparison between canine and human domains. Furthermore, peptides shorter than those previously reported displayed RAD51-interacting activity, and a core motif of this domain consisting of 25 amino acids was identified. Since a mutation (S3323N) was reported in the core motif of this domain, the effect of this mutation was evaluated. The mutant exhibited similar RAD51-binding activity as that of the wild-type protein, suggesting that the mutation was functionally neutral. These data suggested that the C-terminal portion of the BRCA2 C-terminal RAD51-binding domain influenced its RAD51-interaction activity, and a minimum core motif of 25 amino acids was identified in this domain. These data may help clarify BRCA2 function, as well as the tumorigenic effects of BRCA2 mutation.     

单片机技术在自动给料系统中的应用

文章设计了基于单片机控制的送料机自动送料机控制系统,论述了单片机AT89C51在实现其生产过程控制的自动给料系统的设计方法。     

基于STM32单片机的方形喷洒域装置的设计与试验

针对已有方形喷洒域喷头存在喷灌均匀系数低、方形程度差、转动过程精度不高等问题,设计了一种适用于方形喷洒的新型喷灌装置.该装置主要由控制模块、工作模块和喷头组成;通过滚子与空间凸轮的接触,调节喷头仰角,改变射程,实现方形区域的喷洒;采用单片机控制装置的旋转速度,以提高喷灌均匀度.在工作压力0.20 MPa、流量1.30 ...     

一种采用无线数据传输方式的储粮仓群测温系统

基于无线收发芯片和1-Wire总线数字温度传感器,实现了可进行无线数据传输的大型储粮仓群温度监测系统。该系统包括主机和信号采集分机,主机负责管理每个粮仓的温度信号采集分机,定时或根据操作者指令向分机发出数据传输指令,各个粮仓分机根据收到的指令进行温度数据的传输。该系统在继承了全数字粮仓测温系统硬件结构简单、安装及维修方便等优点的基础上,实现了测温数据的无线传输,适于在大型分散式储粮仓群以及不便于布线的粮库中应用。     

6CB-5.4型莲籽穿芯脱壳机控制系统的设计

根据6CB-5.4型莲籽穿芯脱壳机的工作原理设计出了该机的控制系统,该控制系统是基于一台89C51单片机来实现的.为此介绍了系统硬件和软件的设计思想和实现过程,达到了莲籽自动穿芯脱壳的目的,收到了较好的效果.＃     

基于AT89S51的汽车智能安全报警系统

利用AT89S51设计了一种新型汽车防盗报警系统.当满足报警条件,单片机会向GSM模块发出指令,系统向机主终端发出报警求助短信,同时还发出声光报警、切断发动机电源.本系统具有误报率小,可靠性高,成本低,使用方便等优点.     

计算法测量电阻应变原理的试验分析

介绍了计算法测量电阻应变的原理及试验,着重分析了试验结果,验证了测量原理．