基于船舶观测的黑潮延伸体典型涡对的三维结构分析

为了理解黑潮延伸体(KE)区域活跃的中尺度过程,认识该海域中尺度涡旋的三维结构,以及中尺度涡旋在水团再分配、海-气相互作用方面起到的贡献。本文基于船舶观测和卫星遥感数据,研究了2022年6?7月之间分别分布于KE主流轴南北两侧的一个气旋式涡旋(CE)和反气旋式涡旋(AE)构成的典型涡对三维结构特征。结果表明：(1) CE存在南向跨锋运动,剪切强迫主导了该CE的运动过程;AE则相对稳定地存在;(2) CE的“冷舌”结构在海表增温和黑潮暖水扩散作用下逐渐消退;由于对CE所携带冷水的夹带作用,AE表现出“反涡旋海表面温度异常”的结构;(3) 该涡对内部温盐异常的多核结构与不同特性水团垂向再分配作用有关,AE内部流场斜压强,理查德森数小于临界值0.25的占比达50%左右,说明易引发湍流混合;CE上层垂向结构稳定,正压性强,使跨密面混合难以进行。该研究工作有助于提升对KE区域涡对特征和涡旋内部结构的认识,为进一步的涡旋动力研究提供支撑。     

ω-3多不饱和脂肪酸鸡蛋的研究

ω-3多不饱和脂肪酸(以下简称ω-3)在多不饱和脂肪酸的分子中，距羧基最远的双键是在倒数第三个碳原子上。主要是由：C20：5ω-3(EPA)，C22：6ω-3(DHA)，C18：3ω-3(α-亚麻酸)组成。陆地植物油中几乎不含EPA和DHA。海藻与深海鱼中含有较高EPA和DHA。α-亚麻酸进入人体后．经过～系列的脱饱和作用．缓慢而有限度地转变为EPA和DHA，但延伸却比较快。     

SOE PCR重构SLA-Ⅰ复合体研究

利用剪接重叠延伸PCR(SOE PCR)技术,中间加一富含甘氨酸/丝氨酸的linker(G4S)3,将SLA-Ⅰ重链基因SLA-2和轻链基因β2m连接,形成复合体基因链SLA-2-(G4S)3-β2m,然后将复合体基因链克隆入p2X表达质粒,导入受体菌TB1并进行表达。结果显示,利用SOE PCR技术成功得到了复合体基因链SLA-2-(G4S)3-β2m,并且克隆入p2X载体。SDS-PAGE检测表明,复合体基因导入宿主菌后获得了融合蛋白MBP-SLA-2-(G4S)3-β2m,大小为84.1 ku。试验结果为今后在体外进行相关基因重组表达提供参考。     

楚雄州云岭山羊遗传资源调查

云岭山羊是云南省山羊中数量最多,分布最广的地方品种,主产于云南境内云岭山系及其余脉的哀牢山、无量山和乌蒙山延伸地区,故通称为云岭山羊.楚雄云岭山羊具有耐粗饲、耐贫瘠、适应性和抗病力强、早期发育良好、肉质细嫩、味鲜美、板皮厚、皮革细腻等特点.     

山东蚕区桑黄化型萎缩病病原物的分子鉴定

以采自山东省宁阳市桑园的桑黄化型萎缩病发病植株叶脉组织为材料,通过PCR扩增植原体16S rRNA基因及延伸因子基因(tuf)和核糖体蛋白基因(rp),分别得到大小约为1.4、0.8和1.2 kb的目的片段并测定序列。以该病原物的16S rRNA基因与GanBank中相关的植原体16S rRNA基因序列构建系统发育树并进行RFLP分析,结果显示该病原物属于翠菊黄化组的16SⅠr-B亚组,与翠菊黄化组16SⅠr-B亚组典型成员的同源性为99.9%。进一步对延伸因子基因和核糖体蛋白基因构建系统发育树并做RFLP分析,结果显示该病原物与翠菊黄化组的tuⅠf-B亚组典型成员和rpⅠ-B亚组典型成员的同源性分别达到99.6%、99.9%。由此在3个基因水平确定该植原体的分类地位属于16SrⅠ-B、tuⅠf-B和rpⅠ-B亚组。     

Correction of the Mutant SLA-1-632-TPK Gene from ToPigs Pig and Construction of SLA-1-TPK/pMD19-T Recombinant Plasmid

The swine leukocyte antigen (SLA) genes in pigs were the important immune gene group in antigen presentation, and studing on SLA could provide the references for the prevention of some infectious diseases. Earlier studies found that SLA-1-632-TPK gene in ToPigs pig had a deleted base in its coding sequence (a single base "C" was lost in 632 bp from the 5' end of the SLA-1-632-TPK gene), which lead to frameshift mutation. In order to correct the SLA-1-632-TPK gene, two pairs of gene-correction's primers were designed to correct the gene by the splicing overlap extension PCR (SOE-PCR) in template of recombinant plasmid of SLA-1-632-TPK/pMD18-T. Firstly,the 5'and 3'ends of SLA-1-632-TPK gene were amplified, respectively, then both of them were spliced and amplified to form an intact SLA-1-632-TPK gene. After detected by agarose electrophoresis, the interest of the product was further cloned into pMD19-T Simple vector. The positive clones were screened by colony PCR and then sequenced. The result showed that the 5'and 3' ends of the SLA-1-632-TPK gene were all amplified successfully by SOE-PCR, with the products of about 650 and 590 bp, which were consistent with the theoretical value of 648 and 585 bp, respectively. After spliced, the intact sequence of SLA-1-632-TPK gene was obtained with the product of about 1 200 bp, which was close to the theoretical value of 1 223 bp. The colony PCR result showed that the corrected gene was successfully inserted into pMD19-T Simple vector . After the sequence was analyzed by GENETYX version 9.0, it was shown that the nucleotide "C" in 632 bp numbered from the 5'end of the gene was added and the SLA-1-632-TPK gene was coded correctly. In this study, the SLA-1-632-TPK was corrected successfully, and the recombinant plasmid SLA-1-TPK/pMD19-T was constructed, which would lay a foundation to further study the protein expression and associated function of SLA-1-TPK.     

高校档案动态化管理的探索研究

本文通过对高校档案管理方式的分析,对如何将孤立静止的档案通过管理方式的改变和信息技术的应用将这些档案相互关联,使档案具备动态的可延展性和可追溯性,并对其进行了探索研究。     

滑液支原体WVU1853株热不稳定延伸因子的表达及黏附特性分析

旨在探究滑液支原体(Mycoplasma synoviae,MS)热不稳定延伸因子(elongation factor thermo unstable,EF-Tu)的黏附特性.参照GenBank中MS WVU1853株EF-Tu序列,设计引物对Tu-F/Tu-R,利用PCR扩增获得MS EF-Tu基因后,将其克隆入pE...     

禽分枝杆菌副结核亚种重组蛋白r22-ag85B的表达及纯化

为表达并纯化禽分枝杆菌副结核亚种主要抗原基因的串联重组蛋白r22-ag85B,期望研制出一种新型副结核病疫苗,以禽分枝杆菌副结核亚种参考株P18的基因组DNA为模板,扩增了22KD基因和ag85B基因。采用重叠延伸剪接PCR技术（SOE-PCR）获得了融合基因22-agS5B,将基因连接于表达载体pET32a（＋）,构建了重组质粒pET22-ag85B。将其转化到大肠杆菌感受态细胞BL21（DE3）中,以IPTG（终浓度1mmol／L）诱导后对表达产物进行Western blot检测。检测结果显示,成功表达并纯化了重组蛋白r22-ag85B,其分子质量约为65ku。Western blot检测表明此蛋白具有良好的免疫学活性。禽分枝杆菌副结核亚种22-ag85B重组蛋白的成功表达及纯化为副结核病疫苗的研究工作奠定了基础。     

牛GM-CSF与金黄色葡萄球菌黏附素FnBPB共表达质粒的构建与原核表达

采用PCR方法对牛粒细胞-巨噬细胞集落刺激因子(GM-CSF)和金黄色葡萄球菌FnBPB的D区进行特异性的扩增,并通过重叠延伸PCR扩增GM-CSF-FnBPB串联基因,构建了克隆质粒pMD19-GM-CSF-FnBPB。利用表达载体pET-32a(+)对该融合基因片段进行原核表达,SDS-PAGE分析表明,在1mmol/L IPTG诱导浓度下,在39ku处出现了与目的蛋白一致的外源蛋白带,Western blot分析表明,该蛋白具有反应原性,进而证明该融合基因成功在原核细胞中表达。