观赏榆雄孔雀树栖蹲坐造型技艺

挑选2 点高位嫁接金叶垂榆成活1 个接穗的种苗,将白榆和金叶垂榆联合养育成上耸下垂的双色树冠,得到观赏榆作为毛胚树,着眼于成年雄性蓝孔雀整体面貌,抓住蹲坐在树上休息的典型动作,依附孔雀上身金属模具,由中部至上、下两端进行立体加工,采用修剪绑扎技术制作造型,利用绿色叶的白榆创造雄孔雀玲珑的上身,利用金叶垂榆创造雄孔雀硕大的下体,培育出观赏榆雄孔雀树栖蹲坐造型,配植于自然式园林的重要空间,仿佛蓝孔雀入驻植物园。     

BULKED SEGREGANT RNA SEQUENCING (BSR-SEQ) IDENTIFIES A NOVEL ALLELE ASSOCIATED WITH WEEPING TRAITS IN PRUNUS MUME

• Five QTLs associated with weeping traits on chromosome 7 were identified by BSR-seq. • The novel allele PmUGT72B3 has a synonymous transition of T⁶⁶ (upright) to C (weeping) in the coding sequence and a 470-bp deletion in the promoter region. • PmUGT72B3 was associated with hormone and lignin regulation by WGCNA. Weeping species are used both as ornamental plants and for breeding dwarf plant types. However, exploration of casual genes controlling weeping traits is rather limited. Here, we identified individuals with contrasting phenotypes from an F₁ bi-parental mapping population of Prunus mume which was developed from a cross between the upright cultivar ‘Liuban’ and the weeping cultivar ‘Fentai Chuizhi’. Bulked segregant RNA sequencing was used and five QTLs on Chromosome 7 were identified. The Pm024074 (PmUGT72B3) allele, belonging to the UDP-glycosyltransferase superfamily containing the coniferyl-alcohol glucosyltransferase domain, was identified in a genomic region overlapping with a previously identified QTL, and had a synonymous transition of T⁶⁶ (upright) to C (weeping) in the coding sequence and a 470-bp deletion in the promoter region. Pm024074 had exceptionally high expression in buds and stems of weeping P. mume. Weighted correlation network analysis indicates that genes neighboring Pm024074 were significantly associated with plant architecture. In addition, a reliable single nucleotide polymorphism marker was developed based on the variation in the Pm024074 gene, providing precise marker-assisted breeding for weeping traits. This study provides insights into the genetic mechanism governing the weeping trait in P. mume, and indicates potential applications for the manipulation of tree architecture.     

The influence of nitrogen fertilization,spring burning and height of stubble on hay and seed production of eragrostis curvula 1

Abstract

This investigation, conducted over a three‐year period, emphasized the strong effect of nitrogen fertilization on both hay and seed production. The optimum level of nitrogen, for conditions on the Eastern Highveld being 100–150 kg N/ha applied in spring, followed by subsequent dressings dependent on rainfall. Although the influence of spring burning and stubble height on hay and seed production was not as consistent, both had a strong influence under certain conditions and with more research into their action could become important considerations in management strategies for Eragrostis curvula.     

高温胁迫对连翘叶片SOD活性的影响

以连翘为试验材料,研究高温胁迫对连翘细胞SOD活性的影响。研究发现,在12～24h范围内,高温胁迫时间越久,胁迫温度越高,连翘叶片的SOD活性上升的越显著,高温胁迫时间与连翘细胞SOD正相关,相关系数为0.876。     

日本垂枝樱花扦插生根影响因素探讨

在研究园林植物绿枝扦插和组织培养的基础上,利用植物扦插技术对日本垂枝樱花育苗进行探讨。用植物生长类物质处理不同季节的插穗,使其在植物适宜的生长环境条件下快速发根成苗,形成垂樱克隆系。     

Inheritance of a weeping character and the low frequency of rooting from cuttings of the mulberry variety 'Shidareguwa'

A mulberry variety, Morus alba 'Shidareguwa', has a weeping habit and is used as an ornamental and landscape plant. This variety is known for being difficult to propagate by hardwood cuttings. To clarify the mode of inheritance of the weeping character and its relationship to the difficulty in cutting propagation, we crossed 'Shidareguwa' with a non-weeping variety 'Noi' that has a very high rooting ability. The phenotypic segregations exhibited by the F₁ and F₂ plants suggested that the weeping habit is controlled by a single recessive gene. In tests of the F₂ plants for cutting propagation, no obvious correlation was recognized between the weeping trait and the rooting ability of cuttings. As a result, we were able to develop new weeping mulberry strains with improved high rooting ability.     

复方连翘对Th细胞分化信号蛋白钙调神经磷酸酶转录表达的影响

 【目的】观察中药复方连翘对T淋巴细胞活化信号蛋白CaN 转录表达变化，探讨其免疫调控机制。【方法】用含药血清培养鸡脾T淋巴细胞并以OVA和ConA体外干预诱导;利用半定量RT-PCR和Western-blotting技术检测CaN mRNA及其蛋白的表达。【结果】发现信号蛋白CaN转录、表达在0.1% OVA的情况下无血清组和正常血清组高于其它实验组，与10%OVA相比差异显著（P＜0.05）。0.1%OVA浓度下，抗病毒口服液与复方连翘血清组的CaN表达均有明显降低，但两者之间降低程度无显著差别;复方连翘血清组在10%OVA条件下的CaN转录表达，与无血清组比较，有显著升高（P＜0.05）。【结论】复方连翘可能通过调节信号转导通路第一个接头蛋白CaN的转录表达来影响Th的分化进而实现对机体的免疫调控，同时也表明复方连翘通过双向调节、多环节、多途径实现的对机体免疫调节作用。     

垂柳粉对作物幼苗的生长效果

主要研究了垂柳粉对水稻、烟草、玉米等3种作物幼苗的生长效果.结果表明,对水稻幼苗生长较适宜的垂柳粉含量为0.26×10-3 kg/2kg土～0.525×10-3kg/2kg土;对烟草幼苗生长较适宜的垂柳粉含量为0.5×10-3kg/2kg土～1.5×10-3kg/2kg土;对玉米幼苗生长较适宜的垂柳粉含量为0.34×10-3kg/2kg土～0.70×10-3kg/2kg土.     

树干注药对柳树几种保护酶活性及游离脯氨酸含量的影响

以垂柳为材料研究4种注干药剂(吡虫啉、啶虫脒、吡虫啉.敌敌畏和敌畏.氧乐)树干注药后其叶片内几种保护酶活性及游离脯氨酸含量的变化。结果表明:4种药剂树干注药后垂柳叶片内超氧化物歧化酶(SOD)活性均呈现上升-下降-上升的过程。敌畏.氧乐对SOD影响最明显,药后1 d活性上升了85.01%,6 d活性下降了35.99%,20 d上升了32.00%。各药剂处理均可提高多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)的活性,抑制过氧化物酶(POD)活性。吡虫啉对PPO刺激作用最明显,药后10 d活性上升了21.87%;对POD抑制作用最显著,药后6 d活性下降了24.78%。敌畏.氧乐对PAL活性影响最显著,药后6 d活性上升了28.97%。各药剂处理均可提高游离脯氨酸的含量,其中敌畏.氧乐的影响最显著,药后3 d含量增加了52.91%。树干注药30 d后各处理组SOD、POD、PPO和PAL活性及游离脯氨酸的含量均逐渐恢复到对照水平。     

垂柳粉中激素的提取及其对水稻幼苗的发根效果

主要研究了垂柳粉中激素的提取、分离、鉴定及垂柳粉提取液对水稻幼苗发根力的效果.结果表明,垂柳粉中含有我们所熟知的四种植物激素,即IAA、ABA、ZT、GA3;对水稻幼苗发根力较适宜的提取液浓度为0.1×10-6～10×10-6.