植物在水分胁迫下脯氨酸积累的研究─—Ⅳ关于植物体内脯氨酸积累的直接触发因子

依据干旱胁迫下数种植物幼苗体内游离脯氨酸、叶气孔、叶水量的变化，剖析了植物与环境因子、气孔因子、氧气因子、酶因子及能量因子之间的相互依存关系，提出了ＰＲＯ积累系统（ＰＡＳ）。认为水分胁迫下植物体内ＰＲＯ的大量积累是氧化受抑和合成受激共同调控的结果。氧气最有可能为直接触发因子。     

农作物抗旱性鉴定仪的研制和应用

依据陈毓荃等首次提出的连续升温电导法，研制了ＮＫＪ－１型农作物抗旱性鉴定仪，对１４种小麦抗旱性的鉴定结果与田间观测结果一致，也适于果树抗热性鉴定。     

Physiological processes associated with salinity tolerance in an alfalfa half‐sib family

We previously reported an alfalfa half‐sib family, HS‐B, with improved salt tolerance, compared to parental plants, P‐B. In this study, we conducted additional experiments to address potential physiological mechanisms that may contribute to salt tolerance in HS‐B. Vegetatively propagated HS‐B and P‐B plants were treated with a nutrient solution (control) or a nutrient solution containing NaCl (EC = 12 dS/m). Shoots and roots were harvested at various time points after treatment for quantification of proline, soluble sugar, and H₂O₂ using spectrophotometers. Subcellular localization and quantification of Na in roots were conducted using a Na⁺‐specific dye under a confocal microscope. HS‐B produced 86 and 89% greater shoot and root dry biomass, respectively, compared to parental plants, P‐B, under salinity in the greenhouse. Under saline conditions the HS‐B shoots accumulated 115% and roots 55% more soluble sugars than P‐B counterparts. The non‐saline HS‐B shoots, however, showed 72% less soluble sugars than the non‐saline P‐B plants. Under saline conditions HS‐B accumulated 39% less proline in shoots, while roots accumulated 56% more proline, compared to their P‐B parents. HS‐B plants also showed a greater reduction of stomatal conductance under mild saline stress. HS‐B shoots and roots contained 3–4 times less reactive oxygen species (H₂O₂) after salt treatment compared to P‐B plants. Sodium localization and distribution analysis using Na⁺‐specific dye revealed HS‐B plants accumulated 88% and 48% less Na⁺ in stele and xylem vessels compared to P‐B. The study provides insights into the potential mechanisms that may contribute to salt tolerance in HS‐B: maintaining RWC by accumulating soluble sugars while reducing transpiration, maintaining healthy status by reducing oxidative stresses, and preventing salt toxicity by reducing accumulation of Na⁺ inside root cells and xylem.     

Genotypic Variation for Tolerance to Transient Drought During the Reproductive Phase of Brassica rapa

Brassica rapa L. is a genetically diverse parent species of the allotetraploid species, oilseed rape (B. napus) and a potential source of drought tolerance for B. napus. We examined the effect of a 13‐day drought stress period during the early reproductive phase, relative to a well‐watered (WW) control, on subsequent growth and development in nine accessions of B. rapa and one accession of Brassica juncea selected for their wide morphological and genetic diversity. We measured leaf water potential, stomatal conductance, water use, and leaf and bud temperatures during the stress period and aboveground dry weight of total biomass at maturity. Dry weight of seeds and reproductive tissue were not useful measures of drought tolerance due to self‐incompatibility in B. rapa. The relative total biomass (used as the measure of drought tolerance in this study) of the 10 accessions exposed to drought stress ranged from 47 % to 117 % of the WW treatment and was negatively correlated with leaf‐to‐air and bud‐to‐air temperature difference when averaged across the 13‐day stress period. Two wild‐type (B. rapa ssp. sylvestris) accessions had higher relative total and non‐reproductive biomass at maturity and cooler leaves and buds than other types. We conclude that considerable genotypic variation for drought tolerance exists in B. rapa and cooler leaves and buds during a transient drought stress in the early reproductive phase may be a useful screening tool for drought tolerance.     

Physiological Response of Multiple Contrasting Rice (Oryza sativa L.) Cultivars to Suboptimal Temperatures

The physiological response of multiple rice cultivars, eighteen initially and eight cultivars later on, to suboptimal temperatures (ST) conditions was investigated in laboratory and outdoor experimental conditions. Treatment with ST decreased growth in different extents according to the cultivar and affected the PSII performance, determined by chlorophyll fluorescence fast‐transient test, and stomatal conductance, regardless the experimental condition. Two groups of cultivars could be distinguished on the base of their growth and physiological parameters. The group of cultivars presenting higher growths displayed optimal JIP values, and higher instantaneous water use efficiency (WUE_i), due to a lower Gs under ST, unlike cultivars showing lower growth values, which presented worse JIP values and could not adjust their Gs and hence their WUE_i. In this work, we detected at least two cultivars with superior tolerance to ST than the cold tolerant referent Koshihikari. These cultivars could be used as parents or tolerance donors in breeding for new crop varieties. On other hand, positive and significant correlations between data obtained from laboratory and outdoor experiments suggest that laboratory measurements of most of the above mentioned parameters would be useful to predict the response of rice cultivars to ST outdoor.     

Modelling Wheat Stomatal Resistance in Hourly Time Steps from Micrometeorological Variables and Soil Water Status

An accurate estimation of stomatal resistance (r_S) also under drought stress conditions is of pivotal importance for any process‐based prediction of transpiration and the energy budget of real crop canopies and quantification of drought stress. A new model for r_S was developed and parameterized for winter wheat using data from field experiments accounting for the influences of net radiation (R_Net), air temperature (T_Air) and vapour pressure deficit of the atmosphere (VPD) interacting with an average water potential in the rooted soil (ψ_RootedSoil). r_S is simulated with a limiting factor approach as maximum of the metabolic (related to photosynthesis) and hydraulic (related to drought stress) acting influences assuming that, if drought stress occurs, it will dominate stomatal control: r_S = max(r_S(T_Air), r_S(R_Net), r_S(VPD, ψ_RootedSoil)). This transitional approach is suited to reproduce measured daily time courses of r_S with a varying accuracy for the single measurement dates but performed satisfactorily for the whole data set (r² = 0.63, RMSE = 59 s m^?1, EF = 0.60). This new semi‐empiric approach calculates r_S directly from external environmental conditions. Therefore, it can be easily implemented in existing model frameworks as link between operational crop growth models that use the concept of radiation use efficiency instead of mechanistic photosynthesis modelling and soil–vegetation–atmosphere transport models.     

Effects of CO2 Enrichment and Drought on Photosynthesis,Growth and Yield of an Old and a Modern Barley Cultivar

Susceptibility of crops to drought may change under atmospheric CO₂ enrichment. We tested the effects of CO₂ enrichment and drought on the older malting barley cultivar Golden Promise (GP) and the recent variety Bambina (BA). Hypothesizing that CO₂ enrichment mitigates the adverse effects of drought and that GP shows a stronger response to CO₂ enrichment than BA, plants of both cultivars were grown in climate chambers. Optimal and reduced watering levels and two CO₂ concentrations (380 and 550 ppm) were used to investigate photosynthetic parameters, growth and yield. In contrast to expectations, CO₂ increased total plant biomass by 34 % in the modern cultivar while the growth stimulation was not significant in GP. As a reaction to drought, BA showed reduced biomass under elevated CO₂, which was not seen in GP. Grain yield and harvest index (HI) were negatively influenced by drought and increased by CO₂ enrichment. BA formed higher grain yield and had higher water‐use efficiency of grain yield and HI compared to GP. CO₂ fertilization compensated for the negative effect of drought on grain yield and HI, especially in GP. Stomatal conductance proved to be the gas exchange parameter most sensitive to drought. Photosynthetic rate of BA showed more pronounced reaction to drought compared to GP. Overall, BA turned out to respond more intense to changes in water supply and CO₂ enrichment than the older GP.     

The Alleviating Effect of Elevated CO2 on Heat Stress Susceptibility of Two Wheat (Triticum aestivum L.) Cultivars

This study analysed the alleviating effect of elevated CO₂ on stress‐induced decreases in photosynthesis and changes in carbohydrate metabolism in two wheat cultivars (Triticum aestivum L.) of different origin. The plants were grown in ambient (400 μl l^?1) and elevated (800 μl l^?1) CO₂ with a day/night temperature of 15/10 °C. At the growth stages of tillering, booting and anthesis, the plants were subjected to heat stress of 40 °C for three continuous days. Photosynthetic parameters, maximum quantum efficiency of photosystem II (PSII) photochemistry (F_v/F_m) and contents of pigments and carbohydrates in leaves were analysed before and during the stress treatments as well as after 1 day of recovery. Heat stress reduced P_N and F_v/F_m in both wheat cultivars, but plants grown in elevated CO₂ maintained higher P_N and F_v/F_m in comparison with plants grown in ambient CO₂. Heat stress reduced leaf chlorophyll contents and increased leaf sucrose contents in both cultivars grown at ambient and elevated CO₂. The content of hexoses in the leaves increased mainly in the tolerant cultivar in response to the combination of elevated CO₂ and heat stress. The results show that heat stress tolerance in wheat is related to cultivar origin, the phenological stage of the plants and can be alleviated by elevated CO₂. This confirms the complex interrelation between environmental factors and genotypic traits that influence crop performance under various climatic stresses.     

Effect of water deficit in different growth stages on stem sap flux of greenhouse grown pear-jujube tree

The effects of water deficit in different fruit growth stages on the variation of stem sap flux of 6-year old greenhouse-grown pear-jujube trees were investigated. Treatments included sufficient water supply during the whole fruit-growing period (T₁), mild water deficit during the flowering–fruit setting stage (T₂), moderate water deficit during the fruit rapid growth stage (T₃) and severe water deficit during the fruit maturing stage (T₄). Results showed that significant compensation effect on stem sap flux after re-watering was observed in T₂, but not in T₃ and T₄ stages. At the end of rapid growth stage, the diurnal variation of stomatal conductance generally had a similar trend as that of stem sap flux, but with a distinct midday depression from 12:00 to 14:00 p.m. In addition, a linear relationship between the relative available soil water content (RAWC) and the ratio of daily stem sap flux to that of sufficient water treatment was observed (R² = 0.4489).     

遮荫条件下O_3胁迫对冬小麦气孔导度影响的模拟

为了探究在遮荫条件下地表O3浓度增加对冬小麦气孔导度的影响,开展了两种不同强度遮荫与开顶式气室（OTC）相结合的大田试验,设置3个处理组：O3浓度为（100±9）nL.L-1结合（60±5）%遮荫的处理组（T1）;O3浓度为（100±9）nL.L-1结合（20±5）%遮荫的处理组（T2）;O3浓度为（100±9）nL.L-1不作遮荫的处理组（CK）。利用SC-1型气孔导度仪测定了3个处理组冬小麦不同生育期的气孔导度值。综合分析了生育期和O3胁迫,以及水汽压差（VPD）、温度（T）、光照（PAR）环境因子的变化对冬小麦气孔导度的影响,采用修正后的Jarvis非线性气孔导度模型模拟了3个处理组的气孔导度值。结果表明：遮荫对大田环境因子均产生了影响,其中,T1、T2处理组8：00—16：00点的平均温度和水汽压差较CK相比分别下降了5.6℃、4.1℃和0.84kPa、0.74kPa;用该模型得到的气孔导度模拟值与观测值进行了比较,R2=0.88,表明模型模拟效果良好;同时,根据O3吸收通量模型计算出T1、T2和CK处理组在整个O3熏蒸时期,冬小麦O3累积吸收通量分别为14.92、15.52、16.23mmolO3.m-2,并由此建立了O3吸收通量（x）与干物质损失（y）的关系分别为：y=1.0-0.038x和y=1.0-0.022x,表明在相同O3胁迫条件下,遮荫导致了冬小麦O3累积吸收通量的差异,在60%和20%遮荫条件下,O3吸收通量增加10mmol.m-2,与CK相比,其干物质累积损失分别为38.0%和22.0%。