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1.
Cuticular protein gene LmACP8 is involved in wing morphogenesis in the migratory locust,Locusta migratoria
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Cuticular proteins(CPs) are major components of the insect cuticle-associated organs such as integument and wings, although the importance of CPs for wing development and function in hemimetabolous insects remains understudied. In the present study, a wing cuticular protein LmACP8 was identified from Locusta migratoria, which belongs to the RR-2 subfamily of cuticular protein RR consensus(CPR) chitin-binding proteins. LmACP8 was mainly expressed in the wing pads and showed high expression levels before ecdysis of third-, fourth-, and fifth-instar nymphs, with its encoded protein located in the procuticle of wing pads and adult wings. Depletion of LmACP8 by RNA interference markedly reduced the amount of its protein, which consequently caused abnormal wing morphogenesis in the transition from nymph to adult of L. migratoria. We further demonstrated that the abnormal morphogenesis was caused by severe damage of the endocuticle in the wings. LmACP8 was suppressed by 20-hydroxyecdysone(20 E) in vivo, however, its expression was significantly up-regulated after knocking down the hormone receptor gene LmHR39. Thus, the LmACP8 that is negatively regulated by the LmHR39-mediated 20 E signaling pathway is involved in wing development during the nymph to adult transition. 相似文献
2.
Replacing Soybean Meal with Alternative Protein Sources in Diets for Pond‐raised Hybrid Catfish, ♀ Ictalurus punctatus × ♂ Ictalurus furcatus
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Menghe H. Li Brian G. Bosworth Penelope M. Lucas 《Journal of the World Aquaculture Society》2018,49(4):755-760
The present study investigated the replacement of soybean meal with combinations of two or three alternative protein sources in diets for pond‐raised hybrid catfish, ♀ Ictalurus punctatus × ♂ Ictalurus furcatus. Alternative protein sources evaluated included cottonseed meal, distillers dried grains with solubles (DDGS), peanut meal, and porcine meat and bone meal (PMBM). Hybrid catfish fingerlings with a mean initial weight of 35 g/fish were stocked into 25 earthen ponds (0.04 ha) at a density of 14,826 fish/ha. Fish were fed once daily to apparent satiation for 166 d. No significant differences were observed for total diet fed, net yield, weight gain, survival, carcass yield, fillet yield, or fillet proximate composition among dietary treatments. Results show soybean meal may be completely replaced by combinations of cottonseed meal and one or two other alternative protein sources including DDGS, peanut meal, and PMBM in the diet without markedly affecting production and processing characteristics and fillet proximate composition of pond‐raised hybrid catfish. These alternative diets may be used during foodfish production when prices are favorable. 相似文献
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4.
Because vanadium (V) is easily reduced to a cationic form within plant cells, data from resin-extraction of soil were analysed for evidence of interactions between V and the resin-extractable concentrations of magnesium (Mg) and calcium (Ca) on soybean seed yield. Three varieties, 9091, 9061 and 704, were grown over a 3-year period in a corn–soybean–wheat rotation. Surface soil samples (0–15 cm) were extracted with ion-exchange resins, extracts were analysed by inductively coupled plasma methods (ICP), and the results were regressed against seed yield using SAS PROC STEPWISE analysis using forward selection, backward elimination and maximum R2 routines. The seed yield of each variety showed a correlation with a unique set of resin-extractable concentrations of V, phosphorus (P), Mg and Ca, and the V:(V + P), Mg:(Mg + Ca), Mg:(Mg + 1000 V) and Ca:(Ca + 1000 V) ratios. Variety 9091 was most sensitive to the Mg:(Mg + Ca) ratio. Variety 9061 was most sensitive to extractable V and to the V:(V + P) ratio. Variety 704 was sensitive to extractable P, V and Ca and the Mg:(Mg + 1000 V) ratio. For variety 9091, Mg fertilization (not currently practised) may be an economical practice, whereas P fertilization of 704 may not be economical. Each regression technique varied slightly in identification of important factors in seed yield. Concentrations and ratios of resin-extractable elements in soil provide insights into optimal genotype selection and possible management alternatives for a given soil. 相似文献
5.
提出了大豆田恶性杂草—苣荬菜的综合防除技术体系:1)降低大豆在整个轮作体系中的比例,将苣荬菜消灭于禾谷类作物四;2)深翻抑制芽根的萌发;3)大豆前茬作物收获后秋季茎叶处理2.4-D丁酯,最佳用药量为0.81~1.35kg/hm2,最佳施药时期为10月5~10日;4)作为挽救措施可在大豆播后苗前施2.4—D丁酯,用药量为0.72kg/hm2,并可与乙草胺、赛克津等常规大豆田除草剂混用. 相似文献
6.
通过不同品种(系)和密度的超高产栽培试验,分析四因子和二因子的产量效应,各因子对产量贡献的大小为:亩荚数>百粒重>荚粒数。超高产育种和栽培的主攻方向是提高亩荚数,同步增加百粒重。黄淮超高产育种模式:亩荚数46.7±5.3万,百粒重22.4±2.6克,每荚粒数2.18±0.11个。 相似文献
7.
经过蛋白质着色动力学的研究,发现蛋白质着色的行为遵循数学模型Y=aX^b。在pH2-6范围内,蛋白质上染料随染溶pH的下降而上升,在15-30℃温度范围内,蛋白质上染率随温度上升略有提高,当温度超过30℃时,蛋白质上染率逐渐下降。染浴中添加稀土对蛋白质有较强的促染效果,其实质是染浴中蛋白质首先对稀土高价阳离子进行结合,再通过高价阳离子对色素进吸附。 相似文献
8.
青海血蜱不同发育阶段蛋白分析 总被引:2,自引:0,他引:2
考虑到不同发育阶段蛋白多肽及抗原特性方面的差异,本实验利用SDS-PAGE和Westernblotting对青海血蜱的卵、幼蜱、若蜱、成蜱的蛋白进行了分析。SDS-PAGE结果显示,卵出现了7条主带,幼蜱出现了16条主带,若蜱出现了14条主带,成蜱出现了12条主带。它们的蛋白多肽在数量、含量和分子量上存在差异,共同带只有4条,但相邻发育阶段之间的共同带较多。用感染羊血清和免疫羊血清作免疫印迹时,感染羊血清在分子量5.6万处有明显的反应带,免疫羊血清在分子量9.7万处有明显的反应带。 相似文献
9.
甘肃滩羊血红蛋白(Hb)及红细胞蛋白质(Ep)多态性的研究 总被引:6,自引:3,他引:3
运用聚丙烯酰胺凝胶电泳技术(PAGE)对甘肃滩羊产区皋兰、景泰及靖远3县285只滩羊的血红蛋白(Hb)基因座、红细胞蛋白质-1(Ep-1)和红细胞蛋白质-2(Ep-2)基因座多态性进行了检测,结果发现甘肃滩羊Hb基因座存在3种基因型HbAA、HbAB、HbBB,它们受HbA和HbB两个共显性等位基因控制,其中HbB和HbBB在3个群体中均占优势;本研究未在Ep-1和Ep-2基因座上检测到多态性,它们均呈现单态。 相似文献
10.