Analysis of the association among three viruses infecting hop in Australia

Associations among Hop latent virus (HpLV), Hop mosaic virus (HpMV), and Apple mosaic virus (ApMV) were assessed in five hop cultivars at four commercial hop-growing regions in Victoria and Tasmania, Australia. The presence or absence of each virus was confirmed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Spatial patterns of virus-infected plants were characterized using the Spatial Analysis by Distance IndicEs ( sadie ) system of pattern analysis. The association among viruses (occurrence and covariation) was assessed using the Jaccard similarity index, Spearman's rank correlation coefficient, and sadie . The spatial pattern of plants infected by HpLV and HpMV ranged from random to highly aggregated depending upon the cultivar infected and the mean disease incidence. The spatial pattern of plants infected by ApMV was aggregated in six of the seven plots where ApMV was present. A strong positive association between HpLV and HpMV was found in all cultivars at all locations. This association may be the result of the viruses sharing a common aphid vector species, the presence of one virus enhancing the ability of the aphid vector to acquire the other virus either through transencapsidation or influences on virus titre, or mixed infections within source plants. Significant associations, positive or negative, were found less frequently between HpLV and ApMV, and HpMV and ApMV.     

Characterization and PCR-based Typing of Xanthomonas campestris pv. vesicatoria from Peppers and Tomatoes in Serbia

During the last two decades bacterial strains associated with necrotic leaf spots of pepper and tomato fruit spots were collected in Serbia. Twenty-eight strains isolated from pepper and six from tomato were characterized. A study of their physiological and pathological characteristics, and fatty acid composition analysis revealed that all of the strains belong to Xanthomonas campestris pv. vesicatoria. Being non-amylolytic and non-pectolytic, pathogenic on pepper but not on tomato, containing lower amounts of fatty acid 15 : 0 ante–iso, the pepper strains were designated as members of the A group of X. campestris pv. vesicatoria. However, the tomato strains hydrolyzed starch and pectate, caused compatible reactions on tomato but not on pepper, had higher percent of 15 : 0 ante–iso fatty acid, and were classified into B phenotypic group and identified as X. vesicatoria. PCR primers were developed which amplified conserved DNA regions related to the hrp genes of different strains of X. campestris pv. vesicatoria associated with pepper and tomato. Restriction analysis of the PCR product resulted in different patterns and enabled grouping of the strains into four groups. When xanthomonads isolated from pepper and tomato in Serbia were analyzed, they clustered into two groups corresponding to the grouping based on their physiological and pathological characteristics. According to the reaction of pepper and tomato differential varieties, the strains from pepper belong to races P7 and P8 and tomato strains belong to the race T2. All strains were sensitive to copper and streptomycin. Advantages and disadvantages of various bacterial spot management practices are discussed.     

北川白山羊基因组与表型性状相关的多态性RAPD标记

北川白山羊是在特定生态条件下未经系统繁育而形成的优良地方山羊品种,在生长发育、繁殖和抗逆等性状方面具有突出优势。通过对122只北川白山羊个体基因组的扩增,结果从80条RAPD引物中选出12条多态引物,北川白山羊群体平均遗传相似率和遗传多样性指数分别为0.859 8±0.077 6和0.919 8,表明北川白山羊品种具有一定的遗传分化和较丰富的遗传多样性。在12条多态性引物中,SBS06同时对体质量（P=0.028）、体高（P=0.017）和体长（P=0.037）具有显著影响,SBS02对体质量（P=0.033）和体高（P=0.034）具有显著效应,所以推断影响体质量和体高性状的QTL基因座可能与RAPD标记SBS06和SBS02相连锁,可以应用于北川白山羊体质量和体高性状的标记辅助选择以及相应主效基因的进一步研究。     

Major ascaroside pheromone component asc-C5 influences reproductive plasticity among isolates of the invasive species pinewood nematode

Pheromones are communication chemicals and regulatory signals used by animals and represent unique tools for organisms to mediate behaviors and make “decisions” to maximize their fitness. Phenotypic plasticity refers to the innate capacity of a species to tolerate a greater breadth of environmental conditions across which it adapts to improve its survival, reproduction, and fitness. The pinewood nematode, Bursaphelenchus xylophilus, an invasive nematode species, was accidentally introduced from North America into Japan, China, and Europe; however, few studies have investigated its pheromones and phenotypic plasticity as a natural model. Here, we demonstrated a novel phenomenon, in which nematodes under the condition of pheromone presence triggered increased reproduction in invasive strains (JP1, JP2, CN1, CN2, EU1, and EU2), while it simultaneously decreased reproduction in native strains (US1 and US2). The bidirectional effect on fecundity, mediated by presence/absence of pheromones, is henceforth termed pheromone-regulative reproductive plasticity (PRRP). We further found that synthetic ascaroside asc-C5 (ascr#9), the major pheromone component, plays a leading role in PRRP and identified 2 candidate receptor genes, Bxydaf-38 and Bxysrd-10, involved in perceiving asc-C5. These results suggest that plasticity of reproductive responses to pheromones in pinewood nematode may increase its fitness in novel environments following introduction. This opens up a new perspective for invasion biology and presents a novel strategy of invasion, suggesting that pheromones, in addition to their traditional roles in chemical signaling, can influence the reproductive phenotype among native and invasive isolates. In addition, this novel mechanism could broadly explain, through comparative studies of native and invasive populations of animals, a potential underlying factor behind of the success of other biological invasions.     

ON STUDY DIGGED FIELD MOVEMENT''S LAW BY EXPERIMENTAL METHOD OF LASER HOLOGRAPHIC MOIRE INTERFERMETRY

The high-precision displacement measuring technology of optic-measuring mechanics is, for the first time,successfully applied on the similar model of the model land-layer with a new-type variable modulus of elasticity. The angle of movement and the distributed displacement of upper and lower rock-layers are measured. The technology is a combination of laser's technology and the modern chemical sticker-it is called the sticking moire interferometry. Compared with the measured values, the result is very satisfactory.     

Reasoning Method Based on Motorcycle Design

According to the needs of setting up a professional system based on motorcycle design,the CBR(case based reasoning) and RBR(rule based reasoning) methods are introduced.After discussing the difference between the two kinds of reasoning,a method of integrating them into the motorcycle design flow is put forward to realize the design reusable technology and improve the design efficiency,by using the CBR in the step of selecting the similar motorcycle and the RBR in project's modification according to correlative rules.     

Weighting Coefficient Optimization Unification of Lexicographic Method in Fuzzy Multi -objective Optimization Design

The Lexicographic method has subjective and empirical character,so a weighting coefficient is introduced by considering the importance and proportion of each individual object,the preferable result of every individual object can be confirmed by comparing the degree of similarity between the perfect one and the ecumenic one and the Fuzzy optimization will be perfect.The effectiveness of these methods is illustrated by a fuzzy optimal design of the three-bar truss.     

跌水坎流体动力性噪声模拟试验研

以流体声学相似原理为依据，对三种跌水坎形式进行噪声模拟试验。利用古典声学理论对背景噪声进行分析与修正，评估降噪的效果，确定跌水坎的修改形式。并对修改方案进行不同比尺的试验，对声强进行曲线拟合，绘制趋势线，推求原型噪声的大小，为工程建设提供参考依据。     

基于两点机器学习方法的土壤有机质空间分布预测

准确预测土壤有机质(Soil Organic Matter,SOM)空间分布对精细农业、耕地质量建设、生态环境保护以及固碳减排等均具有重要的意义。该研究探讨了基于两点机器学习方法(Two-pointMachineLearning,TPML)提高SOM空间分布预测的可行性。以黑龙江省海伦市为研究区,以气候、地形地貌、社会经济和空间位置信息等因素作为辅助变量,充分利用空间位置信息和属性相似关系,有效处理SOM空间分布异质性及其与辅助变量间关系异质性,以提高TPML方法进行SOM空间分布预测的精度。采用随机森林、基于随机森林的回归克里格、反距离权重法和普通克里格(Ordinary Kriging,OK)方法作为对比,以平均绝对误差(Mean Absolute Error,MAE)、均方根误差(Root Mean Square Error,RMSE)、预测值与真实值相关系数(r)和决定系数(R²)作为评价指标,进行不同样本量下的多组对比试验,评价不同方法的预测精度。结果表明：1)研究区SOM含量在1.775～7.188 g/kg之间,平均值为3.179 g/kg,空间分布...