Age estimation of different tree species using a special kind of an electrically recording resistance drill

The determination of tree age is an important issue for urban green planning, forestry and dendrology; finding non-destructive and quasi-non-destructive methods for this purpose is of great theoretical and practical importance. The resistance drilling method is quasi-non-destructive because the average diameter of an opening that remains after drilling does not exceed 3 mm. Do electrically recording resistance drills allow precise assessment of tree age? The aim of this study is to assess the accuracy of determining the number of tree rings based on an examination of this special kind of drilling resistance profiles for three tree species, the pine Pinus sylvestris L., the oak Quercus robur L., and the birch Betula pendula ROTH. In 2015 and 2016, 15 pine trees, 15 oak trees, and 15 birch trees were randomly selected. For each studied tree, a measurement was conducted using the electrically recording resistance drill IML-Resi E400 with a flat-tipped 1.5/3 mm steel needle (research sample), and an increment core was taken (reference sample). The drill used was not a real Resistograph^®. The analysis of the E400-profiles underestimated the number of tree rings; the mean bias error (MBE) values were –6.5, –2.5, and –6.0 years for pine, oak, and birch, respectively. The proportion of investigated trees with less than five years difference between the research and reference samples varied from 38.4 (birch) to 66.7 (oak) percent. The accuracy of tree age determination was lowest for birch and highest for oak. The binomial generalised linear model (GLM) revealed that the most accurate tree age assessments were obtained from tree rings wider than 2 mm. The measurements clearly showed that the electrically recording resistance drill IML-Resi E400 enables a quick, although approximate, tree age assessment. Future research should concentrate on electronically regulating and recording drills, providing a higher spatial and signal resolution, and a stronger correlation to wood density.     

菠萝蜜种子淀粉制备香草兰微胶囊的工艺研究

以菠萝蜜种子淀粉为壁材,采用饱和水溶液法制备香草兰精油微胶囊,以包埋产率为指标,采用响应面分析法对微胶囊的包埋条件进行优化探讨。结果表明:5个单因素中,影响最显著的因素为壁芯材比例、包埋温度和包埋时间。响应面优化得到香草兰精油微胶囊的最佳工艺条件为壁芯材比例为7.5∶1;包埋时间72 min;包埋温度54℃,此条件下的包埋产率为(95.46±0.2)%,包埋率为(76.35±0.6)%,载油量为(27.73±0.3)%。试验证明,此条件结果与模型预测值相吻合,此工艺条件可为菠萝蜜种子淀粉包埋香草兰精油微胶囊工艺提供理论依据。     

智慧图书馆微观知识生态系统运行机理研究

伴随多元化时代的来临,用户的知识需求日益多元化、专业化,对图书馆服务提出了新的挑战。对智慧图书馆微观知识生态系统运行机理的研究,能够为新型知识服务提供理论基础,保障知识资源进行有效的优化整合,实现服务升级。文章在简要分析智慧图书馆微观知识生态系统四大要素的基础上,着重从社会发展、技术进步,知识富集、优化调整和需求驱动、服务升级三方面探讨其形成动因,重点分析智慧图书馆微观知识生态系统的生命周期,最后从合作、循环和保障三方面探讨其运行机理。     

New Polyketides from the Antarctic Fungus Pseudogymnoascus sp. HSX2#-11

The species Pseudogymnoascus is known as a psychrophilic pathogenic fungus with a ubiquitous distribution in Antarctica. Meanwhile, the study of its secondary metabolites is infrequent. Systematic research of the metabolites of the fungus Pseudogymnoascus sp. HSX2#-11, guided by the method of molecular networking, led to the isolation of one novel polyketide, pseudophenone A (1), along with six known analogs (2–7). The structure of the new compound was elucidated by extensive spectroscopic investigation and single-crystal X-ray diffraction. Pseudophenone A (1) is a dimer of diphenyl ketone and diphenyl ether, and there is only one analog of 1 to the best of our knowledge. Compounds 1 and 2 exhibited antibacterial activities against a panel of strains. This is the first time to use molecular networking to study the metabolic profiles of Antarctica fungi.     

Construction of recombinant capripoxviruses as vaccine vectors for delivering foreign antigens: Methodology and application

Goatpox (GTP), sheeppox (SPP) and lumpy skin disease (LSD) are three severe diseases of goat, sheep and cattle. Their typical clinical symptoms are characterized by vesicles, papules, nodules, pustules and scabs on animal skins. The GTP, SPP and LSD are caused by goatpox virus (GTPV), sheeppox virus (SPPV) and lumpy skin disease virus (LSDV), respectively, all of which belong to the genus Capripoxvirus in the family Poxviridae. Several capripoxvirus (CaPV) isolates have been virulently attenuated through serial passaging in vitro for production of live vaccines. CaPV-based vector systems have been broadly used to construct recombinant vaccines for delivering foreign antigens, many of which have been demonstrated to induce effective immune protections. Homologous recombination is the most commonly used method for constructing recombinant CaPVs. Here, we described a methodology for generation of recombinant CaPVs by the homologous recombination, and further reviewed CaPV-vectored vaccines for delivering foreign antigens.     

Identification of an H1N1 subtype of swine influenza virus and serological analysis

To investigate the epizootic of swine influenza virus(SIV), 60 nasal swabs were collected from a clinical cases of pig farm in Tai'an City, Shandong Province of China in April 2017. SIV was isolated by inoculating into 10-day-old Special Pathogen Free embryonated eggs and the whole genome was sequenced. An H1N1 subtype SIV was isolated and designated as A/swine/Shandong/TA04/2017(H1N1). Phylogenetic analysis showed that apart from the polymerase A(PA) fragment belonging to the 2009 pandemic H1N1 branch, seven genome segments belonged to avian-like H1N1 influenza virus lineage. The cleavage site sequence of the hemagglutinin(HA) protein was PSIQSR↓G, which is a typical molecular biological characteristic. Five potential N-glycosylation sites(N14, N26, N277, N484 and N543) were found in the HA gene. To further investigate the epidemiology of SIV in this farm, the 995 serum samples were assessed with EAH1N1 2009 pandemic H1N1 and H3 N2 antigens. The results showed that the total positive rate was 65.43%. The positive rates of single virus infection detected by EAH1N1, 2009 pdmH1N1 and H3 N2 for serum HI(Hemagglutination inhibition) were 48.35, 30.85 and 7.47%, respectively. The results showed that SIV in Shandong Province has been reassorted in some segments and the SIV-positive rate was high on the SIV outbreak farm. These data provide evidence of an epizootic of SIV.     

橡胶园酸性、中性土壤交换性钙、镁测定方法研究

为了获得简单、易操作、准确度高的橡胶园酸性、中性土壤交换性钙、镁测定方法,本试验通过响应面优化试验,建立称样量、提取液体积、提取方式与交换性钙测定结果之间的数学模型。结果表明：在25℃条件下,以1 mol/L的乙酸铵为土壤浸提液,称样量为2 g、提取液体积175 mL、振荡36 min,为橡胶园酸性、中性土壤的最佳提取条件。将上述条件下得到的结果与标准方法条件下得到的结果相对比,两结果的相对误差在1.25~3.04之间,均属合理范围。利用标准样品对优化方法进行试验验证,结果与推荐值相符。改进后的方法分析成本低、操作简便、测定结果准确、稳定性好,可用于橡胶园酸性、中性土壤交换性钙、镁的测定。     

Differential regulation on C5 expression in goose versus mammals by glucose/palmitate provides a potential protection for goose fatty liver

Goose fatty liver is a specific type of nonalcoholic fatty liver that is protected from harmful effects associated with severe steatosis. Our previous findings suggest that suppression of the complement C5 may be relevant, but the mechanism is unclear. Therefore, in this study, we first verified the expression pattern of complement genes (including C5) during goose fatty liver formation and then determined the liver fat content and fatty acid composition by high-performance liquid chromatography (HPLC), followed by selecting the differential metabolites to treat HepG2, goose and mouse primary hepatocytes, aiming to explore the mechanism of C5 and inflammation suppression in goose fatty liver. The data confirmed the suppression of complement genes (including C5) in goose fatty livers. Moreover, fat content was significantly higher in fatty liver versus normal ones, with oleic acid and palmitic acid dominantly accounting for the difference. In line with this, high concentration of palmitate led to down regulation of C5 expression in goose primary hepatocytes whereas upregulation in mouse primary hepatocytes and HepG2 cells. In conclusion, regulation on C5 expression by fatty liver related factors including high level of palmitic acid may contribute to the protection of goose liver from severe hepatic steatosis.