Intensity Comparative Analysis Based on Ideas Between Involute Gear and Double Involute Gear

Modeler and simulation modules in Ideas software are used to build the finite element models on involute gear and double involute gear with accurate tooth shape, and with the same design parameters and under the same loads, finite element contact analysis has been done. At last, the contact stress and bending stress on the two types gear transmission at the different meshing positions are solved out, and comparative analysis and study have been done. At the same time, that the ladder-shape tooth on the double involute gear how to influence contact stress and bending stress is emphatically analysed. All above offer the theoretical foundation that bring the double involute gear with ladder-shape tooth into the industry.     

淀粉加工用搅拌机PLC控制的改造设计

论述了淀粉加工用搅拌机的工作原理和PLC控制原理,并给出了PLC梯形图。     

Development of a ladder-shape melting temperature isothermal amplification (LMTIA) assay for detection of African swine fever virus (ASFV)

BackgroundDue to the unavailability of an effective vaccine or antiviral drug against the African swine fever virus (ASFV), rapid diagnosis methods are needed to prevent highly contagious African swine fever.ObjectivesThe objective of this study was to establish the ladder-shape melting temperature isothermal amplification (LMTIA) assay for the detection of ASFV.MethodsLMTIA primers were designed with the p72 gene of ASFV as the target, and plasmid pUC57 was used to clone the gene. The LMTIA reaction system was optimized with the plasmid as the positive control, and the performance of the LMTIA assay was compared with that of the commercial real-time polymerase chain reaction (PCR) kit in terms of sensitivity and detection rate using 200 serum samples.ResultsOur results showed that the LMTIA assay could detect the 10⁴ dilution of DNA extracted from the positive reference serum sample, which was the same as that of the commercial real-time PCR kit. The coincidence rate between the two assays was 100%.ConclusionsThe LMTIA assay had high sensitivity, good detection, and simple operation. Thus, it is suitable for facilitating preliminary and cost-effective surveillance for the prevention and control of ASFV.