Pelargonium flower-break and pelargonium line pattern viruses in the Netherlands; purification,antiserum preparation,serological identification,and detection in pelargonium by ELISA

Two viruses, detected frequently in the Netherlands in pelargonium, were identified by serology and test plant reactions. Antisera were prepared and an ELISA procedure was developed to detect the viruses in pelargonium.One of the viruses, PFBV-N, proved to be pelargonium flower-break virus. With the antiserum to PFBV-N, it could be detected reliably throughout the year inPelargonium zonale Springtime Irene.The other virus, PLPV-N, was serologically closely related to pelargonium line pattern virus (PLPV) and to pelargonium ring pattern virus (PRPV), as were an old virus isolate from Saturnus, collected in the Netherlands in 1971 (L128), and PLPV isolates from Yugoslavia (PLPV-Y) and Denmark (PLPV-D). There were only minor differences in host-plant reactions between the virus isolates. Based on these tests, PLPV and PRPV are considered as isolates of the same virus, for which, for practical reasons, the name pelargonium line pattern virus is proposed.PLPV could be reliably detected by ELISA inP. zonale Springtime Irene and Amanda throughout the year with only a few exceptions. InPelargonium peltatum Tavira, however, reslts were erratic due to uneven distribution of virus in the plant. Best results were obtained when petioles of fully expanded leaves were tested.     

Frequency of transmission of artichoke Italian latent nepovirus by Longidorus fasciatus (Nematoda: Longidoridae) from artichoke fields in the Iria and Kandia areas of Argolis in northeast Peloponnesus, Greece

Artichoke Italian latent nepovirus (AILV) transmitted by Longidorus fasciatus is a causative agent of artichoke patchy chlorotic stunting (APCS) in northeast Peloponnesus in southern Greece. Populations of L. fasciatus collected from the Iria and Kandia areas of Argolis in northeast Peloponnesus were used in laboratory experiments to determine the frequency of transmission of the virus by its natural vector. One tenth to almost one half of the L. fasciatus specimens recovered from soil collected in two artichoke fields showing APCS transmitted AILV. Allowing nematodes access for 4 wk to Nicotiana clevelandii mechanically infected with AILV did not increase the number of individual specimens able to transmit virus. The total number of specimens transmitting virus in an experiment did not exceed fifty percent of the individuals tested. Virus-like particles were only observed adsorbed to the inner surface of the odontostyle and it is suggested that the high frequency of transmission of AILV by L. fasciatus is a result of efficient dissociation of virus particles from the specific sites of retention in the vector.     

Postnatal development of canine axial globe length measured by B-scan ultrasonography

OBJECTIVE: The objectives of this study were to document the development of axial globe length (AGL) in normal mesocephalic cross-bred dogs between 2 and 52 weeks of age, to determine a relationship between AGL and age, and derive an equation to predict AGL in normal mesocephalic cross-bred dogs. ANIMALS STUDIED AND PROCEDURE: The AGL of twenty normal mesocephalic cross-bred dogs was measured at 12 time points from 2 to 52 weeks of age using B-scan ultrasonography. RESULTS: The mean (+/- SEM) AGL increased from 12.65 mm (+/- 0.18) at 2 weeks of age to 19.52 mm (+/- 0.18) at 52 weeks of age. The correlation between AGL and age was evaluated by fitting possible variables to a regression pattern. A linear model of natural logarithmic-transformed value of AGL (mm) and age (week) was established. Side (left or right eye) and gender did not correlate with development of AGL. CONCLUSIONS: A reverse transformation of the formula can be used to predict AGL in mesocephalic cross-bred dogs: AGL = 10.847 * age in weeks 0.1653.     

北方半湿润滨海地区海水养殖废水灌溉菊芋研究

2007年3~10月,在山东莱州海洋863中试基地,研究了海水养殖废水灌溉条件下,耐盐能源植物菊芋的盐胁迫响应以及对氮、磷的吸收利用机制。结果表明:海水养殖废水灌溉条件下,菊芋地上部生物量(茎、叶)随着灌溉水盐度(S)的增加明显下降,盐度为9.46 g/L的养殖废水处理生物量仅为淡水处理(CK2)的62.5%;地下部生物量(根、块茎)与盐度之间的关系符合一元二次方程曲线,且当盐度为3.83 g/L时,地下部生物量取得最大值(0.205 kg/株)。养殖废水处理菊芋地上部氮浓度明显低于淡水处理,地下部则各灌溉水处理间氮浓度没有明显差异;磷浓度地上部各灌溉水处理间没有明显差异,但地下部养殖废水灌溉处理明显高于淡水处理,表明海水养殖废水灌溉可以促进菊芋对磷的吸收,但氮利用受到显著影响。通过氮、磷通量计算,进一步证实了这一结果。     

Use of saline aquaculture wastewater to irrigate salt-tolerant Jerusalem artichoke and sunflower in semiarid coastal zones of China

In 2004 and 2005, the feasibility of agricultural use of saline aquaculture wastewater for irrigation of Jerusalem artichoke and sunflower was conducted in the Laizhou region using saline aquaculture wastewater mixed with brackish groundwater at different ratios. Six treatments with different electrical conductivities (EC) were included in the experiment: CK1 (rainfed), CK2 (irrigation with freshwater, EC of 0.02 dS m⁻¹), and saline aquaculture wastewater (EC of 39.2 dS m⁻¹) mixed with brackish groundwater (EC of 4.4 dS m⁻¹) at volumetric ratios of 1:1, 1:2, 1:3, and 1:4 with corresponding EC of 22.0, 16.1, 13.2, and 11.4 dS m⁻¹. Soil electrical conductivity (ECe) in the saline aquaculture wastewater irrigation treatments was significantly higher (P ≤ 0.05) than that in the rainfed or freshwater irrigation treatments, and the maximum value occurred in the 22.0 dS m⁻¹ treatment. The sodium adsorption ratio (SAR) ranged from 4.1 to 11.7 mmol^1/2 L^−1/2 and increased with decreasing salinity of irrigation water. The biomass of Jerusalem artichoke significantly decreased (P ≤ 0.05) when irrigated with saline aquaculture wastewater compared to the rainfed or freshwater irrigation treatments; however, the effect of salinity on root biomass was much smaller than the aerial parts. Concomitantly, the highest tuber yield of Jerusalem artichoke occurred in the 11.4 dS m⁻¹ treatment, while the highest seed yield of sunflower occurred in the rainfed treatment. Additionally, nitrogen and phosphorus concentrations of Jerusalem artichoke were significantly higher in the 11.4 dS m⁻¹ treatment than the other treatments. This study demonstrated that properly diluted saline aquaculture wastewater can be used successfully to irrigate Jerusalem artichoke with higher economic yield and nutrient removal, but not sunflower due to the difference in salt tolerance.     

菊芋耐盐性的初步研究

菊芋为优良耐盐能源植物,但其耐盐程度一直未有定论.为准确探讨菊芋的耐盐性,测定了不同盐胁迫下(电导率(ECe)为0、10、20、30、40、50、60dS/m)菊芋的生长、生理指标.结果表明:在ECe=0～30 dS/m时,菊芋能够正常发芽、生长;但在ECe≥40 dS/m时菊芋不能正常存活;随着NaCl处理时间的延长...     

喷硼对红地球葡萄产量和品质的影响

通过大田对比试验研究了红地球葡萄不同浓度硼和不同处理时期对葡萄产量和品质的影响。结果表明:花中期(50%花朵开放)喷施0.2%的硼有利于提高葡萄的产量和品质,与对照(CK)不喷硼相比,坐果率、单粒重、穗重、单株产量和可溶性固性物含量分别增长了132.47%、24.23%、38.71%、38.71%和30.69%。可滴定酸含量降低了40.82%,均达到极显著水平,且穗形紧凑。可将此方法作为高寒冷凉区设施红地球葡萄增产提质的参考值。     

红地球葡萄主要栽培特性研究

红地球（Ｒｅｄｇｌｏｂｅ）葡萄，系欧亚杂交种，原产于美国加利福尼亚州，是加州的传统栽培品种［１］。该品种在原产地表现为果实粉红色，果穗平均重４５０ｇ，单粒重１５～１８ｇ，果肉脆硬，汁少味甜，可溶性固形物在１７％以上。由于其品质优良，成为风靡世界的著名...     

红地球葡萄日灼病与反光膜套袋初报

在2002年5～7月,用塑料反光膜作外袋、白色葡萄套袋为内袋制成双层反光葡萄套袋,进行红地球葡萄防日灼病的试验研究。结果表明:反光葡萄套袋内温度低于气温0～2℃,当气温为38℃时,该套袋内红地球幼果均未发现日灼现象,防日灼效果显著。