全文获取类型
收费全文 | 11886篇 |
免费 | 881篇 |
国内免费 | 1450篇 |
专业分类
林业 | 898篇 |
农学 | 2550篇 |
基础科学 | 254篇 |
832篇 | |
综合类 | 4700篇 |
农作物 | 932篇 |
水产渔业 | 977篇 |
畜牧兽医 | 1920篇 |
园艺 | 466篇 |
植物保护 | 688篇 |
出版年
2024年 | 46篇 |
2023年 | 163篇 |
2022年 | 357篇 |
2021年 | 393篇 |
2020年 | 432篇 |
2019年 | 482篇 |
2018年 | 366篇 |
2017年 | 495篇 |
2016年 | 542篇 |
2015年 | 526篇 |
2014年 | 579篇 |
2013年 | 602篇 |
2012年 | 843篇 |
2011年 | 849篇 |
2010年 | 762篇 |
2009年 | 755篇 |
2008年 | 726篇 |
2007年 | 730篇 |
2006年 | 655篇 |
2005年 | 533篇 |
2004年 | 462篇 |
2003年 | 416篇 |
2002年 | 327篇 |
2001年 | 320篇 |
2000年 | 239篇 |
1999年 | 226篇 |
1998年 | 184篇 |
1997年 | 154篇 |
1996年 | 170篇 |
1995年 | 147篇 |
1994年 | 110篇 |
1993年 | 103篇 |
1992年 | 104篇 |
1991年 | 85篇 |
1990年 | 84篇 |
1989年 | 50篇 |
1988年 | 38篇 |
1987年 | 37篇 |
1986年 | 25篇 |
1985年 | 8篇 |
1984年 | 12篇 |
1982年 | 10篇 |
1981年 | 12篇 |
1980年 | 4篇 |
1979年 | 9篇 |
1978年 | 9篇 |
1977年 | 9篇 |
1976年 | 4篇 |
1962年 | 6篇 |
1955年 | 11篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
2.
Variability in the performance of juvenile Chinook salmon is explained primarily by when and where they resided in estuarine habitats 下载免费PDF全文
Paul Chittaro Lyndal Johnson David Teel Paul Moran Sean Sol Kate Macneale Richard Zabel 《Ecology of Freshwater Fish》2018,27(3):857-873
Estuarine habitats provide rearing opportunities for the juvenile life stage of anadromous fishes. Because survival is positively correlated with juvenile performance, these estuarine habitats play an important role in population abundance and productivity. To provide information for the recovery of several depressed stocks of Chinook salmon in the Columbia River Basin, we sought to identify the factors that explain variability in performance. Using otolith‐derived estimates of juvenile somatic growth rate as an index of recent performance, we observed a negative nonlinear relationship between growth rate and day of year, and a decreasing and increasing trend of growth rate over the 8 years of this study and distance from the river mouth respectively. Using a generalised linear modelling approach, we found that variability in juvenile somatic growth rate was best explained by where and when individuals were collected, their body size, contaminant loads, stock of origin, and whether a fish was hatchery produced or unmarked. Lastly, we argue that a considerable improvement to the growth rate of juveniles in estuarine habitats is physiologically possible. The results of this 8‐year study provide a baseline of the performance of juvenile Chinook salmon to evaluate habitat restoration programs and to compare against future anthropogenic conditions. 相似文献
3.
为了建立青蒿的SRAP最佳扩增体系,并筛选出SRAP多态性引物,本研究以青蒿叶片DNA为模板,采用正交试验设计,以Mg^2+、dNTP Mix、Taq DNA聚合酶、引物和DNA模板5种因素5个水平,对青蒿SRAP反应体系进行研究。结果表明,青蒿SRAP-PCR最佳反应体系为:引物0.6μmol/L、Mg^2+2.0 mmol/L、模板DNA 5.1 ng、Taq DNA聚合酶2.0 U、dNTPs 0.25 mmol/L,总体积为25μL。各因素对扩增反应均有不同影响,其中引物浓度的影响最大,dNTPs的影响最小。运用该体系对不同种质资源的青蒿进行验证,证明该体系稳定可靠,并在30个引物组合中筛选出了25对扩增条带清晰,多态性丰富的引物组合。这一结论为今后利用SRAP标记技术进行青蒿分子遗传学研究提供了科学依据。 相似文献
4.
Fa-chao SUN Min TAN Yuan-chao ZHANG Yu-chao WANG Sheng-liang CAO Guo-fei DING Fang-yuan CONG Li-hong GUO Si-dang LIU Yi-hong XIAO 《农业科学学报》2019,18(7):1436-1442
To investigate the epizootic of swine influenza virus(SIV), 60 nasal swabs were collected from a clinical cases of pig farm in Tai'an City, Shandong Province of China in April 2017. SIV was isolated by inoculating into 10-day-old Special Pathogen Free embryonated eggs and the whole genome was sequenced. An H1N1 subtype SIV was isolated and designated as A/swine/Shandong/TA04/2017(H1N1). Phylogenetic analysis showed that apart from the polymerase A(PA) fragment belonging to the 2009 pandemic H1N1 branch, seven genome segments belonged to avian-like H1N1 influenza virus lineage. The cleavage site sequence of the hemagglutinin(HA) protein was PSIQSR↓G, which is a typical molecular biological characteristic. Five potential N-glycosylation sites(N14, N26, N277, N484 and N543) were found in the HA gene. To further investigate the epidemiology of SIV in this farm, the 995 serum samples were assessed with EAH1N1 2009 pandemic H1N1 and H3 N2 antigens. The results showed that the total positive rate was 65.43%. The positive rates of single virus infection detected by EAH1N1, 2009 pdmH1N1 and H3 N2 for serum HI(Hemagglutination inhibition) were 48.35, 30.85 and 7.47%, respectively. The results showed that SIV in Shandong Province has been reassorted in some segments and the SIV-positive rate was high on the SIV outbreak farm. These data provide evidence of an epizootic of SIV. 相似文献
5.
提出了一种在线测量混炼胶粘度的新方法.该方法以模糊建模技术为基础,综合考虑混炼过程各因素对胶料粘度的影响,建立起胶料粘度的在线测量模型.在模糊建模中,采用T-S模型描述胶料粘度变化的非线性过程,提出了一种基于相似性判别的模糊聚类算法以自动确定合适的聚类组数目,并用实数编码的遗传算法优化全局参数,从而获得了规则简化的、具有较高精度的模糊模型.根据此方法,设计了测量装置,并进行了现场试验.试验结果表明模糊模型输出与实验室测量值基本一致,平均误差较低且最大误差未超过1门尼.该方法较大地提高了橡胶混炼的生产效率,为粘度最优控制奠定了基础. 相似文献
6.
采用沙黄松天然林自由授粉家系7年生树干材积的材料,分析了联合选择遗传增益结构模式。结果表明:亚群体间存在极显著差异,亚群体内家系间差异不显著;联合选择增益结构模式为亚群体间72.44%、亚群体内家系间3.55%、单株间24.01%。 相似文献
7.
8.
Genetic diversity within and among 20 herbicide-resistant (HR) and 16 herbicide-susceptible (HS) Avena fatua multi-field populations was determined using 82 polymorphic loci resulting from two intersimple sequence repeat (ISSR) primers and one long-primer random amplified polymorphic DNA (LP-RAPD) primer. Collections from the Red River Valley of North Dakota and Minnesota, sampled in 1964 and 2000, represented A. fatua populations before and after intensive exposure to herbicides. A 1995 collection from south-west North Dakota represented A. fatua exposed to low herbicide selection. Despite differences in years of herbicide exposure among collections, both HR and HS populations from every collection maintained nearly similar levels of ISSR and RAPD diversity. Genetic differentiation among populations (GST) varied from 11% to 13% among HR populations and from 9% to 16% among HS populations, indicating that 84–91% of total variation remained within HS or within HR populations. Minimal difference in gene diversity between HR and HS is consistent with multiple origins of resistance, where HR A. fatua most likely evolved from diverse founding individuals. 相似文献
9.
对湖羊生长性状的表型参数和遗传参数的估测表明,性别对这些生长性状有极显著的影响,在遗传参数的估测中剔除性别的效应可提高估计的正确性。初生重、断奶重、三月龄重、六月龄重、周岁重和日增重等性状都属于高遗传力性状,遗传力分别为0.4190,0.3042.0.6023,0.6077,0.3956和0.6945.根据相关分析结果,为提高湖羊的生长速度和肉用性能,把日增重和三月龄重作为主要选择性状,预期可获得较好的效果 相似文献
10.
分子标记用于赤眼蜂分子监测的研究 总被引:5,自引:0,他引:5
通过对松毛虫赤眼蜂(Trichogramma dedrolimi Matsumura)以及玉米螟赤眼蜂(Trichogramma ostriniae Pang et Chen)rDNA-ITS2基因的克隆测序,并联机检索GenBank核酸序列库中其它赤眼蜂的相关序列,利用核酸分析软件找到松毛虫赤眼蜂和玉米螟赤眼蜂ITS2序列中一段有鉴别意义的标志,并据此设计出蜂种的特异PCR引物,实现了松毛虫赤眼蜂和玉米螟赤眼蜂rDNA特异区带的PCR扩增。研究表明该特异区带具有种的特异性。我们认为该分子标记技术可用于目前我国生防中常用的松毛虫赤眼蜂和玉米螟蒌眼蜂的分子监测,如田间种群动态监控和寄生效果评估。 相似文献