EGCG抑制Nicotine诱导肺腺癌H1299细胞JAK2/STAT3 mRNA的表达研究

为探索EGCG对Nicotine诱导肺癌细胞增殖的抑制作用,本研究通过MTT实验筛选出EGCG、Nicotine对肺腺癌细胞H1299的最佳作用浓度,利用实时荧光定量PCR技术检测EGCG、Nicotine对H1299细胞中Bax、Bcl-2、Jak2和Stat3基因mRNA相对表达量的变化。实验结果表明,EGCG对H1299细胞的半抑制浓度IC₅₀值约为32βμmol·L^-1（24βh）、15βμmol·L^-1（48βh）;1βμmol·L^-1的Nicotine对H1299细胞促增殖作用明显;以15βμmol·L^-1的EGCG预处理H1299细胞24βh可显著下调1βμmol·L^-1 Nicotine的促增殖作用（P<0.05）。1βμmol·L^-1的Nicotine处理H1299细胞可明显降低JAK2/STAT3信号通路中Bax基因mRNA的表达,增加Bcl-2、Jak2、Stat3基因的mRNA表达;15βμmol·L^-1 EGCG预处理H1299细胞可反向调控Nicotine诱导所致JAK2/STAT3信号通路中Jak2、Stat3和Bax、Bcl-2基因表达量的变化,结果具有显著性差异（P<0.05）。由此可知,EGCG对Nicotine诱导的肺腺癌H1299细胞增殖及JAK2/STAT3信号通路中促增殖基因mRNA的表达起抑制作用。     

在认知语言学框架下看词语象似性的理据

本文以词汇意义为例,在认知语言学理论的框架下,分析语言符号所具有的基本性质——语言符号的依存性和开放性,进一步探讨词语的象似性的理据。     

关于“增加、提高”意念的常用英文表达式

本文归纳了科技英语中常用的表示“增加、提高”这一概念的6种表达式,并简述它们的使用要则。     

体外培养鸽嗉囊组织形态学、酶活力及基因表达动态变化规律

本试验旨在探究体外培养鸽嗉囊组织形态学、酶活力及基因表达变化规律。取60周龄美国王鸽嗉囊组织于体外培养7 d,动态检测其形态学、代谢和凋亡相关酶活力以及角蛋白和凋亡相关基因表达的变化。形态学结果表明:鸽嗉囊组织于体外培养第2~4天结构清晰,上皮层细胞排列紧密,体外培养5 d后嗉囊结构显著退化,形态学受到破坏。酶活力和基因表达结果显示:Caspase-3酶活力于培养第1、5、6、7天显著高于培养第2~4天(P0.05);琥珀酸脱氢酶、Na~+-K~+-ATP酶、Ca~(2+)-Mg~(2+)-ATP酶和总ATP酶活力以及Bcl-2和角蛋白19基因表达均于培养第1、6、7天显著下调(P0.05),于培养第2~4天显著升高(P0.05);Bak1基因表达于培养第2~5天稳定,随后于培养第6天显著上调(P0.05)。综上所述,体外培养鸽嗉囊组织形态学、酶活力和基因表达随培养时间延长而显著改变。本试验条件下,鸽嗉囊组织体外培养稳定时间为4 d,且开展后续药理、病理及生理学试验前的1 d预培养十分必要。     

试论英语系动词的词汇意义

本文论述了英语系动词的基本词汇意义及其种种细微差异,并列举了某些相关的习惯用法。     

Influence of elevated protein and tannin-rich peanut skin supplementation on growth performance,blood metabolites,carcass traits and immune-related gene expression of grazing meat goats

The aim of the present study was to define whether elevated rumen-undegradable protein (RUP) and tannin-rich peanut skin (PS) supplementation would affect animal growth performance, average daily gain (ADG), blood metabolites, carcass traits associated with lipogenic and immune-related gene expressions in meat goats grazing winter wheat (WW). Thirty-six Kiko-crossbreed male goats at approximately 6 months of age were blocked by body weight (BW; 25.6 ± 1.1 kg) and randomly assigned to one of the four treatments with two replicates based on a 2 × 2 factorial design. Diets contained PS replacing alfalfa meal (ALM), without or with RUP supplementation. Both PS and ALM were incorporated into grain mix portion of the diet and pelletized, with remaining diets fed ad libitum of WW forage for a period of 51 days. Lipogenic genes examined included SCD, ACLY, YWHAZ, PPIA and FABP4, while immune-related genes examined included ACTB (as a control gene), H3F3A, PPIA, IRF3, STAT2, HERC3 and IFIT3 antibody genes. The meat goats on PS-pellet-supplemented group with or without RUP supplementation grew 38.5% faster ADG (p < .001) when compared to control-supplemented group. When goats received PS diet, empty body weight, hot carcass, cold carcass, shoulder, hind shank, rack, loin and fat thickness were greater (p < .05) than control diet. Animals on PS-pellet had higher ACLY, YWHAZ, PPIA and FABP4 gene expression (p < .05) when compared to ALM-pellet control, with RUP by PS-pellet interactions (p < .01). Goats receiving additional RUP supplementation had increased (p < .05) STAT2 gene expression, whereas goats receiving PS-pellet supplementation showed increased STAT2 (p < .05) and a tendency to increase IRF3 (p = .07) gene expressions. In conclusion, the addition of PS-pellet or RUP supplementation has the potential to improve ADG and altered selected lipogenic and immune-related gene expressions.     

英语意义否定句表现法及其汉译琐谈

英语意义否定部分可借助于隐含否定意义的词、词组及惯用句式来表达 ,汉译时要从其内涵着手 ,在理解上下功夫。此外 ,还要注意文化因素所带来的翻译阻碍。     

大学英语教学中的文化输入

语言和文化是密不可分的，注重文化的输入在大学英语的实践教学中尤为重要。通过大学的英语教学受文化制约的分析、课堂教学中文化背景知识的传授、文化特色词语的比较以及在第二课堂教学中文化的导入，阐述了大学英语教学中文化输入的必要性。     

语言简洁摭谈

语言简洁是对对方的尊重,有利于自我形象的塑造,能满足现代社会快节奏、高效率的需要。加强思维训练,讲究遣词造句,恰当使用古语词、成语、熟语,优化句子结构,精心谋篇是实现语言简洁的重要途径。     

How to Achieve Continuity in Writing a Paragraph

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