高校图书馆批量退证系统的设计与实现

针对高校图书馆在毕业生退证高峰期所面临的问题,介绍了惠州学院图书馆批量退证系统的设计目标及思路,并对批量退证系统实现过程中对相关表的解读、数据库的连接方法和功能的实现过程进行了详细的论述。     

Comparison of polymerase chain reaction methods for the detection of <Emphasis Type="Italic">Theileria equi</Emphasis> infection using whole blood compared with pre-extracted DNA samples as PCR templates

Rapid, efficient, and reproducible procedures for isolating DNA before PCR gene amplification are essential for the diagnosis of piroplasms. In this study, we evaluated the ease and reliability of detecting Theileria equi by PCR using pre-extracted DNA samples (by QIAamp DNA Mini Kit and phenol-chloroform methods) compared with blood spotted on FTA cards as PCR templates. Although minimal variations in limit of detection were observed among the methods compared, overall, the use of pre-extracted DNA samples and blood spotted on FTA cards had comparable detection limits. These results indicate that T. equi infection can be efficiently detected directly from FTA cards by PCR without the need for pre-extraction of DNA from blood samples.     

利用贴牌水培法快速鉴定稗草对二氯喹啉酸的抗药性水平

本文通过室内贴牌水培法及整株法,快速鉴定并验证了2013年采自湖南省不同地区稻田的50个稗草生物型对二氯喹啉酸的抗药性,试验结果表明:贴牌水培法所用的二氯喹啉酸甄别剂量为80mg/L;稗草死亡率越低,其抗性倍数(resistance factor,RF)越高,其中死亡率为0时,整株法测定的RF值高达896.07~1 209.38;贴牌水培法测定的稗草样本对二氯喹啉酸的ED50远低于整株法,但两种方法得到的不同稗草生物型对二氯喹啉酸的抗性水平趋势高度吻合。笔者认为以贴牌水培法测定的死亡率小于40%的稗草样本对二氯喹啉酸的抗性风险较高,值得深入研究。     

不同因子对烟粉虱种群控制作用模拟

应用现代控制理论中的状态空间分析方法评价了不同因子烟粉虱种群控制作用的大小,结果如下：黄板诱杀 施用扑虱灵组合对烟粉虱种群的控制作用最佳。烟粉虱种群趋势指数I仅为0．3365,其后依次为黄板诱杀 施用阿维菌素组合、施用吡虫啉、施用阿维菌素、施用扑虱灵、黄板诱杀。在乐果施用区和敌敌畏施用区内,烟粉虱种群出现大量发生和猖獗危害现象。     

Epidemiological and genetical studies in Norwegian pig herds. V. Estimates of heritability and phenotypic correlations of the most common diseases in Norwegian pigs

A genetic analysis was performed on disease registrations from 70 Norwegian pig herds. This analysis was complicated by the small degree of cross classification of dams and sires across herds, and the relatively sparse use of artificial insemination. Herd effects seemed to be responsible for much of the variation in disease frequencies. The estimated heritabilities were relatively low. The results of this investigation also revealed positive phenotypic correlations between the investigated diseases.     

磁卡式管道煤气表计数控制器的研究

控制器配合煤气表的传感装置、磁卡和断气装置，可进行定量计量、报警、断气等功能．多次反复实验结果表明，控制器使用ＣＭＯＳ集成电路，能准确实现清零、预置初值、减计数、声光报警及断气显示等功能．     

不同色彩粘板对麦红吸浆虫成虫的诱集效果比较

为了改进麦红吸浆虫成虫的监测方法,提高监测效率,于2009—2011年在河北省保定市徐水县,通过田间试验比较了14种颜色粘板对麦红吸浆虫成虫的诱集效果,并进行了3个高度的紫板和黄板对该成虫的诱集试验。结果显示：在14种颜色粘板中,紫色、桃粉、橙红、橙黄、浅粉的诱集效果较好,其中以紫板最多,其诱集效果显著高于生产上普遍用于监测的黄板,且紫板上诱集到的蝇类数量也明显偏少;下部粘板诱集到的麦红吸浆虫成虫数量最多,与中、上部之间存在极显著差异,且相同高度的紫板上诱集到的麦红吸浆虫成虫数量显著多于黄板。因此,建议用紫板监测麦红吸浆虫成虫发生动态,高度以紫板下缘与地面平齐为宜。