毛细管电泳及其在药物分析中的应用

阐述了不同操作模式毛细管电泳的分离机制，介绍了其在药物分析中的应用。     

Quantitative capillary reversed passive latex agglutination test for C-reactive protein (CRP) in the dog

A capillary reversed passive latex agglutination test (capillary RPLA) was developed which allows quantification of serum C-reactive protein (CRP) within approximately 15 min. The logarithmic regression line (calibration curve) obtained after measuring each CRP concentration three times in twofold dilutions of a standard canine serum containing 222 g/ml of CRP was y=6.394+0.030x (r=0.995). Capillary RPLA permitted quantification of CRP in the range 6.9–222 g/ml. The coefficients of variation ranged from 10.28% to 12.40%. The recovery rates (percentage recovery) of CRP by capillary RPLA were within the range 87% to 106%. On measuring the CRP concentrations in sera from 78 dogs by capillary RPLA, single radial immunodiffusion (SRID) and enzyme-linked immunosorbent assay (ELISA), close correlations were demonstrated between SRID and capillary RPLA (y=7.250+1.109x, r=0.978), between SRID and ELISA (y=3.042+1.059x, r=0.967), and between capillary RPLA and ELISA (y=1.778+0.929x, r=0.962). Capillary RPLA may be considered useful as a routine biochemical technique for measurement of serum CRP concentration in the dog.Abbreviations CRP C-reactive protein - ELISA enzyme-linked immunosorbent assay - RPLA reversed passive latex agglutination test - SRID single radial immunodiffusion     

意大利瓦勒拉尼整地系统与常规开沟犁整地效益对比分析

通过意大利瓦勒拉尼整地系统（由火车型犁和海豚型犁2种犁型构成,其中火车型犁近似水平沟式整地,海豚型犁近似大鱼鳞坑式整地）与常规开沟犁整地效益的对比试验,结果显示：同期覆土层（0～20 cm）的土壤含水量以瓦勒拉尼系统2种整地方式最高,是常规开沟犁整地的1.28倍和1.11倍。火车型犁整地的土壤容重为1.05 g/cm3,常规开沟犁整地为1.08 g/cm3,火车型犁整地后的土壤容重最小。瓦勒拉尼系统的火车型犁整地后的土壤毛管最大持水量是56.12%,远高于未整地的48.44%和普通犁整地的52.12%,分别是后两者的1.16倍和1.08倍。火车型犁整地后文冠果的地径达1.00 cm,是普通犁整地后地径0.42 cm的2.38倍;树高为0.93 m,是普通犁整地后树高0.53 m的1.75倍;保存率由59.30%提高至91.43%,提高了32.13个百分点;海豚型犁整地文冠果4年保存率则提高了13.42个百分点。     

FEM on Coupled Hydromechanical Dynamic Response of Geogrid Reinforced Soil Retaining Wall

Geogrid reinforced soil retaining wall(GRSRW) is composed of panel,geogrid and soil,its coupled hydromechanical structural characteristics are increasingly emphasized in practice.In this paper,based on the characteristics of the reinforcement-soil interaction,the grogrid and soil around it with a definite thickness were regarded as a kind of composite material with strain-compatible condition.A saturated porous media model was introduced to simulate the soil and reinforcement-soil composite material hereby and the mathematic model for numerical analysis on structural characteristics of GRSRW was built up.The FEM equations were deduced from the standard Galerkin procedure,resolving these equations,we can gain the relevant dynamic response of saturated GRSRW.     

发动机用平板型空滤器流动阻力特性分析和改进

为减小平板型空滤器流动阻力以增大进气量,对平板型空滤器流动阻力特性开展了实验研究,获得了空滤器流动阻力随流量变化的规律和阻力构成成分。阻力随流量的增大而加速增大,滤芯阻力约占整个空滤器阻力的一半,入口流量为120 m 3/h时,总阻力为915.3 Pa,滤芯阻力为426.4 Pa。在实验获得滤芯阻力参数的基础上,提出采用多孔介质跃升模型对平板型空滤器内部流场开展三维数值仿真分析,结果表明,仿真结果与实验结果比较吻合,最大误差为5.67%。滤芯阻力同样约占整个阻力的一半,另一半阻力主要为出口处阻力,其余壁面阻力约占15%。最后,在实验和仿真分析的基础上,提出了改进模型并进行了仿真分析。结果表明,改进模型阻力有较大程度的下降,入口流量为120 m 3/h时,总阻力为588.2 Pa,较原始模型下降了32.2%;增大空滤器流通横截面积是减小阻力以增大进气量的有效手段,改进空滤器壁面的平滑性是补充措施。     

活性多肽提取分离方法研究进展

综述了近几年来生物活性多肽物质的分离和提纯方法,主要包括色谱、电泳等方法,以及这些方法在肽类研究中的应用现状。     

切片多孔介质对流干燥传质模拟软件开发研究

根据非平衡态热力学和相平衡理论,建立了多孔介质对流干燥内部传质模型、传热模型,该模型充分考虑了传热与传质之间的相互藕合关系。用VB编程软件对该模型进行了分析求解,设计开发模拟软件。以切片土豆为例进行传质模拟计算,并与土豆片干燥实验数据进行对比,结果表明:模拟值和实测值十分接近,二者的最大相对偏差小于9.1%。模拟结果显示土豆对流干燥中内部水分由内往外迁移,以及干燥过程中产生的表低内高水分分布规律。     

多孔介质水分运动的随机数值分析

采用van Genuchten-Mualem模型描述非饱和水力传导度和水分特征曲线,将模型中的3个参数作为随机场处理,联合运用Karhunen-Loeve展开和摄动方法,得到饱和非饱和水流的一个随机数值模型。应用该模型分析了介质随机参数的空间变异性对水头分布的影响,结果表明,水头标准差对介质参数的敏感程度由大到小依次为非饱和参数n、β、饱和水力传导度Ks,饱和带水头分布的变异性较非饱和带小。