Basal internode elongation of rice as affected by light intensity and leaf area

Short basal internodes are important for lodging resistance of rice(Oryza sativa L.).Several canopy indices affect the elongation of basal internodes,but uncertainty as to the key factors determining elongation of basal internodes persists.The objectives of this study were(1)to identify key factors affecting the elongation of basal internodes and(2)to establish a quantitative relationship between basal internode length and canopy indices.An inbred rice cultivar,Yinjingruanzhan,was grown in two split-plot field experiments with three N rates(0,75,and 150 kg N ha−1 in early season and 0,90,and 180 kg N ha−1 in late season)as main plots,three seedling densities(16.7,75.0,and 187.5 seedlings m−2)as subplots,and three replications in the 2015 early and late seasons in Guangzhou,China.Light intensity at base of canopy(Lb),light quality as determined from red/far-red light ratio(R/FR),light transmission ratio(LTR),leaf area index(LAI),leaf N concentration(NLV)and final length of second internode(counted from soil surface upward)(FIL)were recorded.Higher N rate and seedling density resulted in significantly longer FIL.FIL was negatively correlated with Lb,LTR,and R/FR(P<0.01)and positively correlated with LAI(P<0.01),but not correlated with NLV(P>0.05).Stepwise linear regression analysis showed that FIL was strongly associated with Lb and LAI(R2=0.82).Heavy N application to pot-grown rice at the beginning of first internode elongation did not change FIL.We conclude that FIL is determined mainly by Lb and LAI at jointing stage.NLV has no direct effect on the elongation of basal internodes.N application indirectly affects FIL by changing LAI and light conditions in the rice canopy.Reducing LAI and improving canopy light transmission at jointing stage can shorten the basal internodes and increase the lodging resistance of rice.     

3种抗倒剂对小麦生长与产量的影响

在小麦生产中,倒伏对其产量影响较大。为达到控高及稳产的目的,选用3种常用的抗倒剂(25%矮壮丰、3%调环酸钙水乳剂、5%抗倒酯水乳剂)来探究其对小麦生长及产量的影响。结果表明,与常规对照R₂(矮壮丰80 m L/667 m²)相比,在灌浆期(5月12日)前,其他抗倒剂处理下小麦的叶绿素含量大都低于对照,而灌浆期后下降缓慢,光合作用时间延长;抗倒剂处理下小麦的株高都低于对照且在R₆(抗倒酯水乳剂60 m L/667 m²)处理下降至最低,且小麦的不同节间长度在抗倒酯水乳剂处理下都得到控制,在最高浓度(60 m L/667 m²)处理下都降至最低;调环酸钙水乳剂仅对小麦的第3、第5节间有控长作用,而抗倒酯水乳剂对小麦的大部分节间都起到控长作用。从产量方面来看,有效穗数在R₆(抗倒酯水乳剂60 m L/667 m²)处理下达到最高,实粒数在不同处理下相差不大,结实率和千粒质量在抗倒酯水乳剂处理下都处于较高水平,但产量在常规对照R₂(矮壮丰80m L/667 m²)处理下最高;其次是R₆(抗倒酯水乳剂60 m L/667 m²)处理。因此,综合考虑控高效果、有效穗数和千粒质量提高及稳产等多种因素,推荐在小麦的分蘖末期喷施60 m L/667 m²的5%抗倒酯水乳剂,能达到控高且不会明显降低其产量的目的。     

Changes in Cell Wall-Bound Phenolic Acids in the Internodes of Submerged Floating Rice

Abstract

Submergence induces rapid elongation of internodes in floating rice (Oryza sativa L.). We examined the distributions of p-coumaric, ferulic and 5-5-coupled diferulic acids ester-linked to cell walls along the axis of highest internodes of submerged and air-grown floating rice stem segments. The amounts of ferulic and 5-5-diferulic acids per cell wall weight were lowest around the intercalary meristem, and increased as the distance from the meristematic zone increased toward the upper part of the internode in both air-grown and submerged stem segments. The ratio of 5-5-diferulic acid to ferulic acid also increased toward the upper, old parts of internodes in both air-grown and submerged stem segments. These observations suggest that the feruloylation of cell wall polysaccharides and the formation of diferulic acids contribute to the cessation of internodal cell elongation and that the formation of diferulic acids in cell walls is controlled by the coupling reaction in addition to the feruloylation. The amounts of p-coumaric acid per unit length and per cell-wall weight were markedly low in the newly elongated region of submerged internodes, and closely correlated with cell-wall dry mass in both air-grown and submerged internodes, suggesting that the deposition of p-coumaric acid in cell walls is related to the formation of secondary cell walls in floating rice internodes.     

氮肥运筹对小麦光明麦1号群体指标及籽粒产量的影响

[目的]筛选适宜中筋小麦光明麦1号高产高效栽培的氮肥运筹方式。[方法]研究不同施氮量和基追比例对光明麦1号群体指标及籽粒产量的影响。[结果]随施氮量增加,光明麦1号群体茎蘖数、籽粒产量呈先上升后下降的趋势。同一施氮水平下,基追比例为5∶5的处理成熟期茎蘖数和茎蘖成穗率最高;籽粒产量也显著高于基追比例为6∶4、7∶3、8∶2的处理。[结论]在该试验条件下,施氮量为300 kg/hm2、基追比例为5∶5时,穗下节间长占总长比例最高,籽粒产量也最高。     

Lodging-related morphological traits of hybrid rice in a tropical irrigated ecosystem

Hybrid rice (Oryza sativa L.) has been proven to be effective in increasing yield potential, but lodging often limits its yield in high-yielding environments. This study was conducted to determine genotypic variation in lodging resistance and lodging-related morphological traits among hybrid and non-hybrid check varieties, and to identify the key morphological traits associated with lodging resistance. Lodging behavior of 16 rice genotypes, including 12 hybrids and four inbreds, was studied at the International Rice Research Institute farm during the 2004 wet season (WS) and 2005 dry season (DS). Grain yield and visual score of lodging were determined at maturity. Lodging-related morphological traits were measured at 30 days after flowering. Large genotypic differences in lodging-related morphological traits were observed. Among these traits, dry weight per unit length, breaking resistance, and lodging index (bending moment/breaking resistance × 100) of lower internodes were significantly correlated with visual score of lodging. Several hybrids demonstrated high lodging resistance although their average plant height was over 120 cm. The lodging-related morphological traits and visual score of lodging in these hybrids were comparable with those of the non-hybrid check varieties with strong lodging resistance. These results suggest that increases in dry weight per unit length and breaking resistance of lower internodes are primary targets for reducing lodging index, thus improving overall lodging resistance of hybrid rice in a breeding program.     

小麦白粉病成株抗性和抗倒伏性及穗下节长度的QTL定位

由小麦品种花培3号和豫麦57杂交获得了DH群体168个株系, 利用305个SSR标记对白粉病成株抗性、抗倒伏性和穗下节长度进行了QTL定位研究。DH群体及两亲本于2005年和2006年种植于山东泰安, 2006年种于安徽宿州。利用基于混合线性模型的QTLNetwork 2.0软件, 共检测到12个加性效应位点和10对上位效应位点。在4D染色体上控制白粉病成株抗性的qApr4D, 贡献率为20.0%, 在各环境中稳定表达, 其抗病等位基因来源于抗病亲本豫麦57; 在7D染色体上控制小麦穗下节长度的qIlbs7D, 贡献率为12.9%, 在各环境中稳定表达。加性效应和上位效应对小麦白粉病成株抗性、抗倒伏性和穗下节长度的遗传起重要作用, 并且基因与环境常常具有互作效应。以上两个QTL可分别用于小麦白粉病成株抗性和穗下节长度的分子标记辅助选择。     

不同蛋白质含量类型小麦的茎型指标分析

为给优质高产小麦的育种和栽培建立茎型指标体系,2002～2003年度和2003～2004年度分别以不同蛋白质含量类型的小麦品种(系)71个和94个为供试材料,测定了株高、各节间长度、籽粒蛋白质含量和产量,研究了不同蛋白质含量类型小麦的株高和各节间长度等茎部性状的差异及其与产量的关系.结果表明,高蛋白质含量类品种的株高、倒2节间长和倒3节间长显著大于低蛋白质含量类型品种;高蛋白高产类型小麦的适宜株高、穗下节间长、倒2节间长和倒3节间长分别为85～107 cm、27 cm 以上、23 cm左右和14 cm左右,在适宜范围内以各项值稍大较为理想;低蛋白质含量、高产类型的品种则分别为85 cm以下、30 cm 以下、18.5 cm左右和12 cm左右,在适宜范围内各项值以稍小较为理想.     

恒山以北地区油松大树移植及管护实践

总结了国内对油松移植的相关研究,通过对恒山以北地区市镇绿化油松大树移植、管护及成活、生长情况的观察,结合当地气候及环境,分析节间生长量、土球完整性、移植季节、污染物对油松大树移植质量的影响,以期为提高今后该地区油松移植质量提供参考。     

Analysis of Successive Internode Growth in Sweet Sorghum Using Leaf Number as a Plant Age Indicator

Abstract

In sweet sorghum, which is a potential biomass crop, the diameter of internode is an important component of stem yield. However, the thickening of successive internodes is poorly understood. The objective of this study was to clarify the relationship between the thickening of successive internodes using the age indicated by the leaf number on the main stem (AL) as a time scale. Furthermore, the relationship between the elongating and thickening period of successive internodes along the stem was analyzed. Internodes were collected from AL3, when the 3rd leaf had just expanded above the 2nd leaf sheath, to AL17, and at 9 weeks after heading as final harvest. Although the internode thickening patterns based on AL could not be combined into one or a few patterns, a significant correlation (P<0.001) was found between internode position and AL at which the internodes were thickening, suggesting that a regularity of internode thickening existed among successive internodes. The higher the internode position, the longer the first half of the thickening period (from 15% to 50% of thickening), whereas the period of the second half (50%–85% thickening) was constant irrespective of internode position. These results suggest that the degree of the elongation and thickening of successive internodes can be estimated by using AL.     

Identification of Casparian Bands in the Mesocotyl and Lower Internodes of Rice (Oryza sativa L.) Seedlings Using Fluorescence Microscopy

Abstract

The mesocotyl and lower internodes of seedlings are underground organs that play a possible role in water absorption and transport. The aim of this study is to reexamine and understand further the anatomy and morphology of mesocotyls and lower internodes in rice, especially in terms of the existence of Casparian bands. Anatomical structures of mesocotyl and lower internodes tissue of rice seedlings were observed by light and fluorescence microscopy. The rice mesocotyl had two central cylinders, one is large and the other small, in the cross section. Casparian bands were observed in both the endodermis and exodermis of the mesocotyl, and also in the endodermis of the first internode. Furthermore, some bundles with intermediate xylem differentiation were observed between the exarch and endarch arrangement in the first internodes. Casparian bands were identified in the radial walls of cells surrounding each of these bundles. The second internode is quite similar to that of upper internodes in adult plants with respect to internal structure; scattered vascular bundles were seen as in monocotyledons. In the second internode, Casparian bands were seen in the radial cell walls of the bundle sheaths in each vascular bundle. Unlike the mesocotyl, Casparian bands were not observed in the hypodermis of the first or second internodes. The results show that the histological features of the mesocotyl and lower internodes of rice seedlings widely differed. In addition, the present study provides anatomical evidence for the existence of Casparian bands in both the mesocotyl and lower internodes of rice seedlings.