不同激素对金花茶圈枝繁殖生根的影响

分析NAA、IBA及2种激素等体积混合溶液对金花茶圈枝繁殖生根成活率的影响。结果表明:采用萘乙酸和吲哚丁酸的等体积混合激素溶液1 000~1 500 mg/L能明显提高金花茶圈枝繁殖苗的生根率,达到95%以上。该技术简化了操作程序,可节省人力,在基质方面也节约了生产成本,可直接应用于金花茶苗木大规模生产。     

NAA和IBA对福建茶扦插生根的影响

用300 mg/L NAA和300 mg/L IBA处理福建茶1 h、2 h、3 h、4 h,用600 mg/L NAA和600 mg/L IBA处理福建茶1 min、5 min、10 min、20 min,60 d起苗观察生根情况。结果表明,300 mg/LNAA和300 mg/L IBA处理福建茶,NAA处理3 h和IBA处理4 h效果最好,生根率83%和87%;600 mg/L NAA和600 mg/L IBA处理福建茶,NAA处理5 min和IBA处理10 min效果最好,生根率83%和90%。     

Regeneration of adventitious shoots from mature stored cotyledons of Japanese plum (Prunus salicina Lind1)

Adventitious shoot regeneration from mature stored cotyledons of Japanese plum (Prunus salicina Lind1) was achieved in vitro. The influences of the presence and absence of the light, different concentrations of thidiazuron (TDZ) and benzyladenine (BA) in the culture media, TDZ pretreatments and different basal salts on shoot regeneration were evaluated. TDZ was more effective in inducing shoot regeneration from mature stored cotyledons than BA. Dark incubation significantly increased the regeneration frequencies. Quoirin/Lepoivre (QL) basal salts stimulated shoot regeneration more than woody plant (WPM) or B5 salts did. The frequency of adventitious shoot formation varied among the varieties and the regeneration ability appeared to be genotype depended. The frequency of regeneration under the optimum tested conditions for ‘Bruce’, ‘Shiro’, ‘Redheart’, ‘Gladstone’ and ‘Early Golden’ cotyledons were 66.7%, 46.7%, 43.3%, 26.7% and 6.7%, respectively.     

In vitro high frequency direct plant regeneration from whole leaves of blackberry

High frequency and direct (without callus) plant regeneration was achieved from whole leaf explants of thornless blackberry (Rubus hybrid) cv. Black Satin (EC No. 381258; PI No. 553272) in vitro. Leaf blade explants from 1-, 3- and 5-month-old mother cultures were cultured on Murashige and Skoog (MS) medium with thidiazuron (TDZ), N⁶-benzylaminopurine (BAP), indol-3-butyric acid (IBA) and α-naphthalene acetic acid (NAA), alone or in combination. Three-month explants cultured on 0.02 mg l⁻¹ TDZ produced a high regeneration frequency (91.7%) and the most shoots/leaf explant (17.3). The shoot primordia developed within 3 weeks from the point of detachment of the petiole from the leaf blade. The age of the explant source significantly affected the shoot regeneration potential of the leaf explants. Leaves excised from 3-month-old in vitro-cultured shoots performed better than those from 1- and 5-month-old shoots. Shoots rooted best on half-strength MS basal medium with 0.5 mg l⁻¹ IBA and 90% of the plantlets survived acclimatization. The regenerated plantlets were morphologically similar to the mother plants.     

IBA处理对杂交榛根蘖育苗生根指标及相关氧化酶活性的影响

以"龙榛1号"当年萌生条为试材,研究了不同IBA浓度处理(500、750、1 000、1 250mg·L^-1)对平欧杂交榛根蘖育苗生根的影响,分析了根蘖育苗生根过程中过氧化物酶(POD)、多酚氧化酶(PPO)活性的变化,并对外源激素处理与相关氧化酶活性与生根的关系进行了相关性分析,以期探讨其生根机理。结果表明:IBA浓度处理能显著地提高生根率,以1 000mg·L^-1的生根效果最佳,生根率和不定根质量均显著高于其它处理。生根过程中,POD和PPO活性呈先上升后下降趋势,POD活性在育苗后20d出现峰值;PPO活性在40d时达到峰值。生根率与POD、PPO活性呈极显著正相关。说明适宜浓度IBA能增加生根过程POD、PPO活性,从而提高生根率。     

猕猴桃组培苗生根培养的研究

[目的]为建立和完善猕猴桃快速繁殖体系提供理论依据。[方法]以美味猕猴桃无根组培苗为试材,研究培养基、IBA浓度和活性炭对其生根的影响。[结果]1/2MS培养基是最适合诱导猕猴桃组培苗生根的基本培养基,生根率、平均生根数分别是MS培养基的5.05、6.46倍。当IBA浓度为1.0mg/L时,猕猴桃组培苗的生根率为84.4%,生根数为3.82条/株,根平均长度为1.96cm,且植株生长健壮。活性炭对猕猴桃组培苗生根有明显的促进作用,当培养基中加入0.3%的活性炭时,猕猴桃组培苗的生根率达95.6%,生根数达4.53条/株,根平均长度达2.67cm。[结论]猕猴桃组培苗最适宜的生根培养基为1/2MS培养基+IBA1.0mg/L+0.3%活性炭。     

北碚榕扦插繁殖技术研究北碚榕扦插繁殖技术研究

[目的]得出北培榕扦插繁殖技术最优的生根处理方式。[方法]研究了激素种类、处理浓度、处理时间、插穗长度以及基质种类对北培榕扦插生根的影响。[结果]插穗的最优长度为10 cm,用1 000 mg/L的IBA溶液处理15 s生根率可达96.7%。总体来看,IBA处理插穗的效果比NAA要好,根系较发达;扦插基质以珍珠岩：腐殖土为2∶1最好。[结论]扦插成活的难易程度除了和植物本身特性有关,还与处理插穗的激素种类、浓度、时间等有关。插穗的长度、扦插季节以及枝条的粗细对插穗生根也有很大影响。     

三种生根剂对"广益"牛鞭草插穗生根的作用

本试验以NAA、ABT、IBA三种生根剂各200×10-6,100×10-6,80×10-6,60×10-6,40×10-6浓度对"广益"牛鞭草插穗处理5分钟,通过测定株平均生根数、株平均生根长度、最大根长及其分蘖情况,说明80×10-6的ABT、100×10-6的IBA、200×10-6的NAA能使牛鞭草插穗的生根数分别增加72%、318%、254%,根长分别增加103%、724%、548%,鲜根量分别增加88%、128%、88%。证明生根剂对牛鞭草插穗成活生根有促进作用,应在生产实践中推广运用。     

中华猕猴桃单芽绿枝扦插试验

中华猴猕桃单芽绿枝扦插试验结果表明:1.采自组织培养的猕猴桃植株的插穗比采自嫁接植株的插穗更易生根,且发根早,根量大;2.插穗经不同浓度的吲哚丁酸(IBA)处理后可不同程度地促进生根,处理以100ppm浸泡插穗基部20小时为最佳.生根率可达100%.     

6-BA和IBA对丹波黑豆愈伤组织黄酮产量影响研究

以丹波黑豆下胚轴为外植体,接种到1/2MSN6(MS无机盐 N6有机盐)上培养出一致的愈伤组织,将一定量的愈伤组织接种到不同浓度的6-BA(6-苄氨基腺嘌呤)和IBA(吲哆-3-丁酸)组合的培养基上培养15天后,称重,计算愈伤组织生物产量,并取出烘干至恒重,称量其干重。提取愈伤组织中黄酮,分别测定芦丁和槲皮素的生物产量。结果表明:(1)2mg/L的6-BA处理,愈伤组织产量最高,高于其他浓度下的产量,6-BA与IBA相互作用对愈伤组织生物产量有显著影响;(2)2mg/L的6-BA处理,芦丁生物产量和含量最高,并显著高于其他浓度下的芦丁生物产量;(3)2mg/L的6-BA处理,槲皮素生物产量和含量最高,并且其槲皮素生物产量随培养基中IBA浓度的增加而降低;(4)愈伤组织生物产量与芦丁黄酮和槲皮素黄酮生物产量都存在显著的正相关;(5)愈伤组织中槲皮素生物产量和芦丁生物产量之间也存在着显著的正相关。