云南醉鱼草属(Buddleja L.) 观赏植物资源的调查研究

 在对云南分布的醉鱼草属观赏资源系统调查和资料整理的基础上, 研究了该属植物在云南的地理分布规律及25 种(包括变种) 醉鱼草的观赏性状, 包括花、叶、香气等观赏特征及其开花期, 指出开发利用面对的问题和应采取的措施。     

云南金丝桃属植物资源的调查研究

 通过对云南分布的金丝桃属植物资源系统调查和资料整理，研究了该属植物在云南的地理分布及24种金丝桃的观赏性状及其资源状况，提出其持续合理开发利用的建议及措施。     

基于改进UNet模型的油茶果振动采摘点定位方法

针对非花果同期油茶果采收效率低这一问题,提出一种侧枝振动采摘点定位方法,通过振动侧枝降低树木损伤并实现高效采收。首先构建数据集,对侧枝分段标注,向UNet中添加CloFormer注意力机制并命名为Clo-UNet,实现侧枝的二维重构。其次,在Clo-UNet基础上进一步设计采摘点定位方法并命名为Clo-UNet-Point,该方法优先选择采收离果实最远且最粗的枝条。试验表明,Clo-UNet在验证集上表现优异,其中br_con（连果枝）、danger（危险区）和br_pro（优先采收区域）的平均交并比mIoU分别达到85.36%、86.37%和81.29%,平均像素精度mPA分别达到94.97%、96.17%和89.48%,Clo-UNet在整个数据集上的mIoU和mPA分别比UNet高5.14、6.85个百分点。通过观察验证集647幅图像,Clo-UNet-Point算法在不同光照条件下均能定位到采摘点,平均检测一张图像用时0.15 s。该研究可为未来非花果同期类油茶果的自动化振动采收奠定理论基础。     

基于近红外光谱技术的茶油原产地快速鉴别

为研究茶油原产地溯源问题,维护其市场秩序,促进公平竞争。该文利用近红外光谱技术采集湖南、江西、安徽和浙江4个不同产地茶油的光谱数据,并运用 Savitzky-Golay 平滑（savitzky-golay, SG）、多元散射校正（multiplicative scatter correction, MSC）、一阶导数（first derivation, FD）和矢量归一化（vector normalization, VN）等4种方法对其进行预处理。采用偏最小二乘法（partial least squares, PLS）提取最佳主成分,构建 PLS 回归模型;同时,采用主成分分析（principal component analysis, PCA）和 PLS 算法提取最佳主成分,作为 BP 人工神经网络（BP artificial neural network, BPANN）输入变量,构建 PCA-BPANN 和 PLS-BPANN 模型。以验证集相关系数 RP 和验证集均方根误差 RMSEP 为模型的评价指标,分别优选最佳 PLS 和 BPANN 模型。试验结果表明,SG-PLS-DA 和 SG-PLS-BPANN-DA 模型对未知样本的整体分类准确率均大于90%。其中,SG-PLS-BPANN-DA 的鉴别效果优于前者,其建模集相关系数 RC、均方根误差 RMSEC 分别为0.974、0.170,验证集相关系数 RP、均方根误差 RMSEP 分别为0.972、0.172,对上述两类样本集的总体分类准确率分别为98.15%、95.83%,该模型能较准确鉴别茶油原产地。研究结果可为快速辨别茶油原产地提供参考。     

烟属野生种主要植物学性状及黑胫病抗性评价

鉴定了烟属植物野生种生育期等植物学性状特性,评价了供试材料对黑胫病(0号及1号生理小种)的抗性,为开展远缘杂交花期相遇及亲本选配提供了基础数据。在玻璃温室内,本研究针对66份烟属植物野生种的生育期等7个植物学性状的调查,检测了烟碱及钾含量,并对黑胫病(0号及1号生理小种)抗性进行了评价。结果表明:供试材料播种到开花时间(DF)为35.00~357.00 d,平均121.67 d;种子千粒重(TSW)为0.02~0.20 g,平均0.09 g;株高(PH)为11.20~292.75 cm,平均119.19 cm;花冠长度(CL)为1.22~11.50 cm,平均3.98 cm;花冠直径(CD)0.67~7.80 cm,平均2.37 cm;最大叶长(MLL)为6.79~61.15 cm,平均25.17 cm;最大叶宽(MLW)为0.65~35.51 cm,平均11.84 cm。烟碱含量为0.03%~1.81%,平均0.34%;钾含量为0.32%~7.11%,平均3.56%。筛选到N.alata PI42334等11份资源同时抗黑胫病0及1号生理小种。     

基于ADAMS的油茶果采摘机械臂试验设计及优化

针对目前我国油茶采摘主要以人工为主,劳动强度大、采摘效率低等问题,设计了一种适合园艺化栽培的履带式油茶果采摘机,并在Adams软件中对机械臂进行试验设计和优化仿真。试验数据表明:油茶果采摘机工作区间与各机械臂的长度和液压缸安装距离呈线性相关,最佳组合处的机械臂长度总和减小25.67%,可为油茶果采摘机的机械臂设计提供参考。     

油茶幼林结实与果枝特性研究

通过对油茶自花授粉全同胞子代、无性系间控制授粉全同胞子代、半同胞子代和无性系四种不同群体的结果枝特性和开花结实特点等主要经济性状的研究,揭示了四种群体及其优良林分的结实特性和产量规律,为油茶杂交组合、优良家系和无性系等良种的生产应用和栽培技术、以及进一步开展杂交和选择育种研究积累了必要的技术和理论知识。     

毕节市油茶产业发展现状及对策

油茶作为世界四大木本食用油料植物之一,在林业产业和山区经济发展中占有十分重要的 地位。近年来,毕节将油茶产业作为助推脱贫攻坚的重要支柱产业之一,大力发展。文章介绍了毕节 市近年推进油茶产业的一些主要做法,并指出了当前发展油茶产业存在的一些问题,针对问题,提供 了现阶段的一些对策,以期为促进全市油茶产业发展,助推脱贫攻坚提供一定的理论依据。     

Identification of Rosellinia species as producers of cyclodepsipeptide PF1022 A and resurrection of the genus Dematophora as inferred from polythetic taxonomy

Rosellinia (Xylariaceae) is a large, cosmopolitan genus comprising over 130 species that have been defined based mainly on the morphology of their sexual morphs. The genus comprises both lignicolous and saprotrophic species that are frequently isolated as endophytes from healthy host plants, and important plant pathogens. In order to evaluate the utility of molecular phylogeny and secondary metabolite profiling to achieve a better basis for their classification, a set of strains was selected for a multi-locus phylogeny inferred from a combination of the sequences of the internal transcribed spacer region (ITS), the large subunit (LSU) of the nuclear rDNA, beta-tubulin (TUB2) and the second largest subunit of the RNA polymerase II (RPB2). Concurrently, various strains were surveyed for production of secondary metabolites. Metabolite profiling relied on methods with high performance liquid chromatography with diode array and mass spectrometric detection (HPLC-DAD/MS) as well as preparative isolation of the major components after re-fermentation followed by structure elucidation using nuclear magnetic resonance (NMR) spectroscopy and high resolution mass spectrometry (HR-MS). Two new and nine known isopimarane diterpenoids were identified during our mycochemical studies of two selected Dematophora strains and the metabolites were tested for biological activity. In addition, the nematicidal cyclodepsipeptide PF1022 A was purified and identified from a culture of Rosellinia corticium, which is the first time that this endophyte-derived drug precursor has been identified unambiguously from an ascospore-derived isolate of a Rosellinia species. While the results of this first HPLC profiling were largely inconclusive regarding the utility of secondary metabolites as genus-specific chemotaxonomic markers, the phylogeny clearly showed that species featuring a dematophora-like asexual morph were included in a well-defined clade, for which the genus Dematophora is resurrected. Dematophora now comprises all previously known important plant pathogens in the genus such as D. arcuata, D. bunodes, D. necatrix and D. pepo, while Rosellinia s. str. comprises those species that are known to have a geniculosporium-like or nodulisporium-like asexual morph, or where the asexual morph remains unknown. The extensive morphological studies of L.E. Petrini served as a basis to transfer several further species from Rosellinia to Dematophora, based on the morphology of their asexual morphs. However, most species of Rosellinia and allies still need to be recollected in fresh state, cultured, and studied for their morphology and their phylogenetic affinities before the infrageneric relationships can be clarified.     

油用山茶属植物育种与利用研究进展

山茶油是我国南方地区特有的木本油料树种,有非常悠久的栽培历史.文章综述了油用山茶属植物良种选育的研究现状,包括常规育种、杂交育种及分子辅助育种,并对油茶的营养成分、油茶油的加工工艺和油茶副产品的综合利用的现状作了概述.最后展望了非食用山茶植物油作为生物能源开发的前景.