On the Analysis of EMCS and Its Application in the Project of Shenzhen Underground

In this paper, the selection of type, function and technological requirements of EMCS for Shenzhen Underground has been introduced.Based on the investigation for EMCS's worldwide applications and the analysis of practice in Shenzhen Underground, the comparison and selection of PLC and DDC have been done completely.At the same time,the introduction of EMCS is organized by the center level, station level and the spot level.     

基于FPGA的数字下变频设计与实现

数字下变频是全数字解调器中的关键技术之一,其性能好坏直接决定解调器的工作性能。给出一种基于FPGA的数字下变频设计,详细介绍正交变换、CIC抽取滤波及根升余弦滚降FIR低通滤波器的原理设计,并可编程设置各个模块参数。自动生成及动态配置滤波器系数。该设计在Xilinx公司XC3S4000 FPGA芯片的硬件平台和ISE9．2开发环境下,采用Verilog语言编程实现,经过实际通信系统验证,在全数字解调器中很好地完成了多载波、多速率信号的数字下变频处理功能,具有很强的灵活性、稳定性和可扩展性。     

虫酰肼对斜纹夜蛾幼虫多巴脱羧酶基因表达的影响

【目的】克隆斜纹夜蛾（Spodoptera litura）多巴脱羧酶（dopa decarboxylase,DDC）基因cDNA全长,研究虫酰肼对表皮形成过程中多巴脱羧酶基因表达的影响。【方法】通过RT-PCR和RACE技术克隆得到斜纹夜蛾多巴脱羧酶基因的cDNA全长;通过生物信息学工具对斜纹夜蛾多巴脱羧酶基因的DNA序列及其编码的蛋白质序列进行分析;采用饲料浸毒法测定虫酰肼对斜纹夜蛾不同龄期幼虫的毒力;利用荧光实时定量PCR检测用亚致死剂量虫酰肼处理的斜纹夜蛾幼虫DDC基因的表达水平。【结果】克隆了斜纹夜蛾多巴脱羧酶基因的cDNA全长,开放阅读框为1 263 bp,编码420个氨基酸,5′端非编码区长105 bp,3′端非编码区长79 bp。克隆得到的cDNA全长提交至GenBank,登录号为KF620492。多重序列比对及系统进化树显示斜纹夜蛾DDC基因推导的氨基酸序列与鳞翅目昆虫的DDC序列具有很高的相似性。利用SWISS-MODEL在线工具进行同源建模,成功预测斜纹夜蛾DDC三维结构,其与果蝇属（Drosophila）的多巴脱羧酶空间结构具有很高的相似性,功能结构域具有良好的保守性。测定虫酰肼对斜纹夜蛾3、4、5和6龄幼虫的毒力,其LC50分别为33.32、40.28、71.20和71.97 mg•L-1。DDC转录表达结果显示,用虫酰肼分别处理12-48 h,4个亚致死剂量（7.50、16.24、28.34和45.63 mg•L-1）诱导的DDC表达水平均显著高于对照,随着处理时间的延长激活作用呈现出先上升后下降的趋势,其中16.24 mg•L-1亚致死剂量在24、36和48 h诱导表达水平最高,各处理36 h表达水平最高。处理60 h,4个亚致死剂量的表达水平均显著低于对照。这一结果说明虫酰肼进入虫体后先启动基因表达,而后抑制表达,影响昆虫正常蜕皮。【结论】克隆得到斜纹夜蛾多巴脱羧酶基因cDNA全长,虫酰肼干扰昆虫幼虫蜕皮与其影响表皮形成过程中多巴脱羧酶基因的表达有关。     

七波段黑碳气溶胶监测仪的设计与实现

为了实现黑碳气溶胶的实时测量与远程监控,提出了一种七波段黑碳气溶胶监测仪的设计与实现方法。利用电荷数字模数转换芯片DDC101实现信号的采集,使用触摸屏提供人机交互界面,提供网络远程监控与历史数据导出功能。结果表明,系统可以满足黑碳气溶胶长期连续监测实际应用需求,后续数据分析处理方便。     

DDC与DCS集散型控制系统

讨论了DCS的特性,掌握DDC的输入和输出的连接,DDC控制的原理和方法。     

Changes of Tight Junction Protein Claudins in Small Intestine and Kidney Tissues of Mice Fed a DDC Diet

DDC (3,5-diethoxycarbonyl-1,4-dihydrocollidine)-fed mice are widely used as a model for cholestatic liver disease. We examined the expression of tight junction protein claudin subspecies by immunofluorescent histochemistry in small intestine and kidney tissues of mice fed a DDC diet for 12 weeks. In the small intestine, decreases in claudin-3, claudin-7 and claudin-15 were observed in villous epithelial cells corresponding to the severity of histological changes while leaving the abundance of these claudin subspecies unchanged in crypt cells. Nevertheless, the proliferative activity of intestinal crypt cells measured by immunohistochemistry for Ki-67 decreased in the mice fed the DDC diet compared with that of control mice. These results suggest the possibility that DDC feeding affects the barrier function of villous epithelial cells and thus inhibits the proliferative activity of crypt epithelial cells. On the other hand, in the kidney, remarkable changes were found in the subcellular localization of claudin subspecies in a segment-specific manner, although histological changes of renal epithelial cells were quite minimal. These results indicate that immunohistochemistry for claudin subspecies can serve as a useful tool for detecting minute functional alterations of intestinal and renal epithelial cells.     

ALA处理对遮荫下西瓜幼苗叶绿素荧光参数的影响

用100 mg/L 5 - 氨基乙酰丙酸(5-aminolevulinic acid, ALA) 处理遮荫条件下的西瓜幼苗叶片10 d后, 用PAM22100型便携式叶绿素荧光仪连续测定其叶绿素荧光特性。结果表明, 遮荫显著降低叶片光化学效率, 而ALA处理提高弱光下幼苗叶片PSⅡ实际光化学效率(ΦPSⅡ) 、表观光合电子传递速率( ETR) 、光化学猝灭系数( qP ) 以及光化学反应能量分配率( Pc) 。SOD活性抑制剂二乙基二硫代氨基甲酸钠(DDC) 处理降低ΦPSⅡ、ETR、qP和Pc值等荧光参数, 而ALA处理可以消除DDC的抑制效应, 说明ALA对PSⅡ光化学反应的促进效应与细胞抗氧化酶活性有关。遮荫处理提高叶片初始荧光( Fo) , 降低PSⅡ最大光化学效率( Fv/Fm) , 而遮荫下ALA和(或) DDC处理对这些荧光参数变化没有明显效应。据此推测, ALA处理10 d时对西瓜叶片PSⅡ本身没有直接影响, 它对光化学效率的促进效应可能是通过增强PSⅠ附近SOD等抗氧化酶活性, 促进活性氧代谢来实现的。     

《中图法》与DDC编订机制及其比较研究

以《中图法》和DDC作为研究对象,分别介绍了两法的编订组织、经费来源、修订方法和修订频率,在此基础上对两法的异同点进行比较研究。     

Creation of an Experimental System for Testing the Dynamic Tensile Stress of Cells Using Membrane Variable Stress Wave

The constitution, design ideas and working principles of the DDC experimental system for testing dynamic tensile stress of outside body cell using membrane variable stress wave are discussed in detail. A new design measure is put forward, i.e. using airtight liquid for transferring pressure, and a space mechanism which is made up of two moveable linking axes and controlled by DDC for producing dynamic tensile stress with variable stress wave. The space mechanism is made up of two moveable linking axes, the first axis is controlled by an alternative current servo system, at the same time it drives the modulating modulating disc, and its rotation velocity is uniform and adjustable, so the rotating velocity of the disc can be controlled. Second, the hydraulic pressure worktable is driven by step electric motor. By dynamically changing the relative position of the modulating disc and the worktable, the journey of the piston in hydraulic pressure vat can be changed accordingly. The resultant motion of the modulating disc rotation and the radial direction relative movement of the piston can create liquid pressure with variable frequency and amplitude range, and the pressure can be changed into membrane tensile stress with variable frequency and pressure amplitude range using pressure fine tuning, and muhi-path disporting pressure. The system is proved to be simple, reliable, economic and controlled expediently by the experiment conducted.