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排序方式: 共有658条查询结果,搜索用时 125 毫秒
1.
Cytochrome P450 monoxygenase converts arachionic acid to four epoxyeicosatrienoic acid regiosomes: 5, 6-EET (epoxyeicosatrienoic acid); 8, 9-EET; 11, 12-EET and 14, 15-EET. Recent studies show that EETs are involved in signal transduction. EETs open Ca2+-sensit ive K+ channel and inhibit Na+ channel, Ca2+-sensitive Cl- channel and so on. What is more, EETs have been demonstrated to activate PP60c-src and initiate a tyrosine kinase cascade that mediates mitogenic effects. 相似文献
2.
Troy D Anderson 《Pesticide biochemistry and physiology》2004,80(1):54-64
The acute toxicities of two organophosphorodithioate (dimethoate and disulfoton) and two organophosphorothioate (omethoate and demeton-S-methyl) insecticides were evaluated individually and in binary combination with the herbicide atrazine using fourth-instar larvae of the aquatic midge, Chironomus tentans. Atrazine alone up to 1000 μg/L did not show significant toxicity to the midges in a 48-h bioassay. However, atrazine concentrations as low as 1 μg/L in combination with dimethoate at EC25 (concentration to affect 25% of tested midges), 100 μg/L in combination with disulfoton (EC25), and 10 μg/L in combination with demeton-S-methyl (EC25) significantly enhanced the toxicity of each organophosphate insecticide. In contrast, atrazine concentrations of 10 μg/L and above in combination with omethoate (EC25) significantly decreased the toxicity of the insecticide. Biochemical analysis indicated that increased toxicity of dimethoate, disulfoton, and demeton-S-methyl in binary combination with atrazine correlated to the increased inhibition of acetylcholinesterase. Furthermore, cytochrome P450-dependent O-deethylation activity in the midges exposed to atrazine at 1000 μg/L was 1.5-fold higher than that in the control midges. Thus, atrazine appeared to induce cytochrome P450 monooxygenases in the midges. Elevated cytochrome P450 monooxygenase activity may increase the toxicities of dimethoate, disulfoton, and demeton-S-methyl by enhancing the oxidative activation of dimethoate into omethoate, and disulfoton and demeton-S-methyl into their sulfoxide analogs with increased anticholinesterase activity. In contrast, atrazine reduced the toxicity of omethoate possibly by enhancing the oxidative metabolic detoxification since omethoate does not require oxidative activation. 相似文献
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试验研究了五倍子、复方抗菌剂对鲫鱼CYP3A酶活性的影响。设计复方抗菌剂浓度分别为30mg/kg、10 mg/kg和五倍子浓度为30 mg/kg,采用灌肠给药对鲫鱼进行处理;在试验的第3、7、10天取样测定其肝和肾组织中CYP3A的酶活性变化。结果表明:复方抗菌剂30 mg/kg处理组和五倍子30 mg/kg处理组的肝CYP3A活性较对照组显著降低(P<0.05),且在处理第10天,肝CYP3A酶活性较前2次的降低极显著(P<0.01);而复方抗菌剂10 mg/kg组的肝CYP3A酶活性无显著变化。对于肾组织,各药物组的CYP3A酶活性均无明显变化。 相似文献
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YAN Xiao-cheng MU Wei-na QIANG Ye ZHAO Hui-chen SUN Qi YAO Xiao-min ZHANG Yu-chao LIU Yuan-tao 《园艺学报》2000,36(9):1661-1666
AIM To investigate the effects of cytochrome P450 (CYP450) epoxygenase/epoxyeicosatrienoic acid (EET) pathway on insulin resistance in obese mice, and to explore the possible mechanisms. METHODS High-fat diet-induced obesity model was established in C57BL/6Cnc mice, and the obese mice were randomly divided into 3 groups, including obesity group (treated with saline; n =10), EET group (treated with 11,12-EET; n =10) and EET inhibitor 14,15-epoxyeicosa-5(Z)-enoic acid (EEZE) group (n =10). Normal C57BL/6Cnc mice (n =10) treated with saline served as control. Protein expression of CYP2J2 (one of CYP450 epoxygenases) and hypoxia-inducible factor-1α (HIF-1α) was measured by Western blot. Vessel-like structure was detected by immunofluorescence staining. The serum levels of insulin, tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6 and monocyte chemoattractant protein-1 (MCP-1) were measured by ELISA. RESULTS In obese mice, homeostasis model assessment of insulin resistance (HOMA-IR) values were increased, the protein level of CYP2J2 was reduced, and the protein level of HIF-1α was increased in adipose tissues as compared with the controls (P <0.05). The serum levels of MCP-1, IL-1β, IL-6 and TNF-α were also significantly increased in obese mice (P <0.05). After treatment with 11, 12-EET, the HOMA-IR values were decreased compared with vehicle-treated obese mice, HIF-1α expression levels were decreased in the adipose tissue, and the serum levels of MCP-1, IL-1β, IL-6 and TNF-α were reduced (P <0.05). Immunohistochemical results of adipose tissue from vehicle-treated obese mice showed a marked decrease in vessel-like structures (CD31-positive) compared with normal control mice (P <0.05). EET treatment significantly increased the newly formed vessel-like structures in the visceral adipose tissues of obese mice as compared with vehicle-treated obese mice (P <0.05). CONCLUSION High-fat diet-induced obesity and insulin resistance are closely related to the CYP450 pathway. Exogenous EETs effectively decrease obesity-induced insulin resistance possibly through pro-angiogenesis and attenuation of hypoxia and inflammation. 相似文献
6.
害虫对新烟碱类杀虫剂的抗药性及其治理策略 总被引:18,自引:4,他引:14
烟碱和新烟碱类杀虫剂都是作为后突触烟碱乙酰胆碱受体(nAChRs)的激动剂作用于昆虫中枢神经系统,但这两类杀虫剂存在明显不同的选择毒性:烟碱类对哺乳动物毒性较高,而杀虫活性低;新烟碱类具有高杀虫活性,而对哺乳动物低毒。由于新烟碱类杀虫剂的作用方式独特,对以前使用的如拟除虫菊酯类、氯化烃类、有机磷类和氨基甲酸酯类等杀虫剂很少或无交互抗性,该类杀虫剂为防治一些世界性重大害虫(包括对以前使用的杀虫剂具有长期抗性的害虫)作出了重要贡献。但现已发现不少害虫对新烟碱类杀虫剂产生了抗性。文章就害虫对新烟碱类杀虫剂的抗性概况、抗性机理和抗性治理策略进行了综述。 相似文献
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为揭示西花蓟马Frankliniella occidentalis对温度的适应机制,采用高通量测序技术对低温(-13℃)、常温(26℃)和高温(40℃)胁迫1 h后西花蓟马进行转录组测序分析,筛选差异表达的unigenes,并对其进行功能注释与相关代谢通路富集分析,同时随机选择8个西花蓟马耐热性相关unigenes进行实时荧光定量(real-time fluorescence quantification PCR,qRT-PCR)测定,以验证转录组测序数据的正确性。结果显示,转录组测序、组装后共获得79 516个unigenes,在NR和KEGG数据库中分别注释到28 675个和47 285个unigenes。40℃与26℃温度胁迫后差异表达的unigenes数量最多,为333个;-13℃与26℃温度胁迫后差异表达的unigenes最少,只有134个;40℃与-13℃温度胁迫后差异表达的unigenes为230个。差异表达的unigenes主要参与内质网蛋白合成和代谢通路等途径,其中有许多热激蛋白基因和细胞色素P450基因受到高低温胁迫后上调表达,表明这些unigenes可能参与西花蓟马应对极端温度的耐受性作用。随机筛选的8个差异表达unigenes的qRT-PCR结果与转录组测序结果一致,表明转录组数据可靠。 相似文献
10.
Heather K. Knych Carrie J. Finno Russell Baden Rick M. Arthur Daniel S. McKemie 《Journal of veterinary pharmacology and therapeutics》2021,44(1):36-46
The in vivo metabolism and pharmacokinetics of flunixin meglumine and phenylbutazone have been extensively characterized; however, there are no published reports describing the in vitro metabolism, specifically the enzymes responsible for the biotransformation of these compounds in horses. Due to their widespread use and, therefore, increased potential for drug–drug interactions and widespread differences in drug disposition, this study aims to build on the limited current knowledge regarding P450‐mediated metabolism in horses. Drugs were incubated with equine liver microsomes and a panel of recombinant equine P450s. Incubation of phenylbutazone in microsomes generated oxyphenbutazone and gamma‐hydroxy phenylbutazone. Microsomal incubations with flunixin meglumine generated 5‐OH flunixin, with a kinetic profile suggestive of substrate inhibition. In recombinant P450 assays, equine CYP3A97 was the only enzyme capable of generating oxyphenbutazone while several members of the equine CYP3A family and CYP1A1 were capable of catalyzing the biotransformation of flunixin to 5‐OH flunixin. Flunixin meglumine metabolism by CYP1A1 and CYP3A93 showed a profile characteristic of biphasic kinetics, suggesting two substrate binding sites. The current study identifies specific enzymes responsible for the metabolism of two NSAIDs in horses and provides the basis for future study of drug–drug interactions and identification of reasons for varying pharmacokinetics between horses. 相似文献