Lipoprotein electrophoresis differentiation of chylous and nonchylous pleural effusions in dogs and cats and its correlation with pleural effusion triglyceride concentration

Pleural effusions from 23 dogs and 25 cats hospitalized between November 1986 and April 1987 at the Veterinary Hospital of the University of Pennsylvania (VHUP) were classified as chylous (chylomicrons present) or nonchylous (chylomicrons absent) by the presence or absence, respectively, of a chylomicron band on lipoprotein electrophoresis gels of the effusions. Triglyceride concentrations, cholesterol concentrations, and cholesterol/triglyceride (C/T) ratios were compared between the chylous and nonchylous groups in each species. Cholesterol concentrations were not significantly different between chylous and nonchylous effusions in both dogs and cats. Cholesterol/triglyceride ratios of less than 1 were present in all dogs and cats with chylous effusions; however, 12% of dogs with nonchylous effusions and 50% of cats with nonchylous effusions also had a C/T ratio less than 1. Triglyceride concentrations accurately classified all effusions as chylous or nonchylous in both dogs and cats. Pleural effusions with triglyceride concentrations greater than l00 mg/dl were chylous in all cases, whereas, effusions with triglyceride concentrations less than l00 mg/dl were nonchylous in all cases.     

Effects of Medium-chain Triglyceride on Growth Performance and Serum Biochemical Indices in Broilers

The study was conducted to investigate the effects of medium-chain triglyceride replacing the soybean oil in the basal diet on growth performance and serum biochemical indices of broilers.One hundred and forty four healthy,similar body weights AA broilers at the age of 14 days were randomly assigned to four groups with six replicates ger group and six broilers per replicate.Groups A,B,C and D were respectively fed with 0,0.6%,1.2% and 2% medium-chain triglyceride substituted the equivalent amount of soybean oil (using corn flour to balance the energy levels in each group) .The test lasted for 28 days and the indexes were measured once every two weeks.The results showed that the average daily gain and average daily feed intake of groups B,C and D were higher than that of group A,and the F/G was lower than group A.The average daily gain of groups C and D at 28 to 42 days old and group D at 14 to 42 days old were significantly higher than that of group A (P < 0.05).The serum glucose,total content of amino acid,total protein and albumin of groups B,C and D at age of 28 and 42 days old were higher than that of group A,while the serum triglyceride,total cholesterol and urea nitrogen levels were lower.The total cholesterol content at 42 days old and triglyceride concentrations at 28 days old of group C were significantly lower than those of group A (P < 0.05).The results indicated that medium-chain triglyceride could raise average daily gain and reduce the total cholesterol content and triglyceride concentrations of broilers,which were useful for improving the growth performance.     

高产和低产奶牛几项血清生化指标的比较

对高产和低产奶牛(305天泌乳期的产乳量分别为6000公斤以上和4000公斤以下)的几项血液生化指标进行比较研究,结果表明:高产奶牛在血清总蛋白、球蛋白、血糖,谷草转氨酶活性值等方面,显著高于低产奶牛(p<0.005),而血清钙、磷、甘油三酯的含量及谷丙转氨酶活性值等,则低于低产奶牛,但差异不显著(p>0.005)。     

不同剂量粗毛黄褐孔菌的血糖调节作用对比分析

分析不同剂量粗毛黄褐孔菌的血糖调节作用,定量分析粗毛黄褐孔菌药用功效,为医学实践提供指导。采用SPSS19.0统计分析软件,结合描述性统计分析方法分析不同剂量粗毛黄褐孔菌的血糖调节作用,构建实验的参考组和对比组,经过使用不同剂量的粗毛黄褐孔菌,为期3个月进行血糖的跟踪测试,以甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)等为检测指标,采用分组样本回归分析检测方法,进行血糖调节作用的模型组合分析,采用定量递归分析方法测试血糖调节作用与粗毛黄褐孔菌食用剂量的关联性关系。粗毛黄褐孔菌对血糖调节作用有显著性影响,在显著度为0.95的水平上满足收敛性条件,通过定量食用粗毛黄褐孔菌,改善血糖功能。该模型能定量分析不同剂量粗毛黄褐孔菌对血糖的调节作用,分析结果准确可靠,在医学药用实践中具有很好的应用性。     

超微粉碎茶粉对猪生长和胴体品质的影响

为了研究超微粉碎茶粉对育肥猪生长和胴体品质的影响,选用240头平均体重65 kg的杜大长三元杂交猪,分别饲喂添加超微粉碎茶粉0,05,10和20 g·kg-1饲料。试验为期40天,试验开始和结束时,每头猪分别称重,计算增重、平均日增重、耗料和耗料增重比。于试验结束日采用piglog 105 猪胴体瘦肉率超声波测定仪检测猪背膘厚度、眼肌面积和瘦肉率。每组选取体重相近的试验猪6头(3公3母)进行屠宰,采血样,测定胴体长、各部位背膘厚度和眼肌面积。在自动生化分析仪上测定血糖、血清甘油三酯、胆固醇和尿素氮。结果表明: 添加不同量的超微粉碎茶粉对猪的生长没有显著影响;添加茶粉后活体估测胸腰结合部背膘厚分别降低119 mm(P>005),342 mm(P<005)和410 mm(P<005),眼肌面积分别提高970%,870%和1007%(P<005),瘦肉率也有不同程度的提高;屠宰试验,除胸腰结合部(P<005)外,添加茶粉对其他部位的背膘没有显著影响(P>005),眼肌面积有不同程度的提高;血液指标测定,添加茶粉后降低了血脂和胆固醇(其中20 g·kg-1添加组,P<005),而血糖和尿素氮含量不显著受影响。试验结果表明,添加超微粉碎茶粉在不影响猪增重的情况下,一定程度改善猪的胴体品质,其作用机理可能与降低血脂有关。     

去势术对雄性中华田园犬脂代谢的影响

[目的] 研究去势术对雄性中华田园犬脂代谢的影响。[方法] 将12只年龄、体重相近的中华田园犬分为非手术组和去势组,对去势组中华田园犬进行去势手术,观察并测定术前第1天、术后第14天、术后第21天血清中甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白（LDL）、高密度脂蛋白（HDL）含量,以非手术组作为对照,分析去势术对雄性犬脂代谢的影响。[结果] 去势组与非手术组相比,血清中TG含量在第14天到第21天显著上升;血清中TC含量相比非手术组呈下降趋势;HDL第14天和第21天都低于非手术组;LDL都高于非手术组,整体呈上升趋势。[结论] 去势手术会影响雄性中华田园犬机体脂类代谢,会导致去势犬甘油三酯升高,总胆固醇和高密度脂蛋白下降,低密度脂蛋白上升。     

Nutritional management of hyperlipaemia

Hyperlipaemia is a disease resulting from excessive mobilisation of triglyceride stores such that plasma clearance processes become overwhelmed. Consequently increased plasma triglyceride concentrations (>5.6 mmol/l), visibly cloudy plasma and a sick, anorexic or hypophagic subject follow. Epidemiological studies have identified many predisposing and triggering factors and the attentive carer or veterinary surgeon should be alert to such risk factors so that the disease can be prevented, or at least identified and treated at an early stage. Hyperlipaemic subjects are invariably in a negative energy balance, and nutritional management therefore plays a central role in both the prevention and resolution of the disease.     

Changes in density of under-yearling masu salmon <Emphasis Type="Italic">Oncorhynchus masou</Emphasis> in a natural stream after release

ABSTRACT:   The relation between the density of fish and parameters such as body weight, condition factor and the hepatic triglyceride (TG) content of masu salmon Oncorhynchus masou juveniles after release into a natural stream were studied. Hatchery-reared juveniles of different sizes and numbers were released in May 1999, 2000 and 2001, and investigations were carried out from July to September each year. The density of fish was relatively high in 1999, when juveniles of the largest size and number were released, but then continued to decrease during the investigation. The hepatic TG content remained considerably low in 1999, and there was a significant negative correlation between the density of fish and the hepatic TG content of the juveniles caught over three years. The initial fish size and number at release as well as the nutritional condition of the juveniles may affect the density of fish after release.     

Effects of dietary lipids on the fatty acid composition of triglycerides and phospholipids in tissues of white sturgeon

Eight purified diets were fed to juvenile white sturgeon, Acipenser transmontanus Rick, for 9 weeks to investigate the effect of dietary lipids on the fatty acid composition of phospholipids and triglycerides from muscle, liver and brain. The diets contained 150 g kg^?1 of oils from canola, corn, cod liver, lard, linseed, soybean, safflower, or a control mixture (corn oil/cod liver oil/lard, 1:1:1, by wt). Dietary lipids significantly (P≤ 05) affected the composition of tissue triglycerides and phospholipids. Tissue triglyceride fatty acid composition ranged widely, in parallel with the dietary lipids, while phospholipids changes were more conservative. Brain phospholipid fatty acid composition was less responsive to diet compared with that in muscle and liver. Considerable amounts of n-6 and n-3 long chain polyun-saturated fatty acids (> C₂₀) were found in triglycerides and phospholipids with all diets, demonstrating that white sturgeon can desaturate and elongate linoleic acid (18:2n–6) and linolenic acid (18:3n–3). Further, the products of the Δ6 desaturase, i.e. 18:3 n–6 and 18:4n–3, were relatively abundant in triglyceride, suggesting that the Δ6 desaturase might not be a limiting step in the process in white sturgeon. Nevertheless, accumulation of both EPA and DHA was greater in the sturgeon fed fish oil than those fed linseed oil, indicating that muscle triglyceride EPA and DHA levels are best enhanced by diets rich in preformed EPA and DHA.     

Congenital lipoprotein lipase deficiency in hyperlipemic kitten siblings.

The nature of the hyperlipemia in two 3-week-old male kittens, one of which was presented with the owner's complaints of retarded growth and white streaks on its eyes, was studied. Hypertriglyceridemia, hypercholesterolemia, and reduced Post-Heparin Plasma Lipolytic Activities (PHPLA) were observed in both animals. One of the kittens, however, was more severely affected and in addition, had lipemia retinalis and a marked lactescent, hyperchylomicronemic serum in spite of a short-term fast before sampling. These findings are strikingly similar to those found in human Type I hyperlipoproteinemia due to familial Lipoprotein Lipase (LPL) deficiency. Diagnosis of persistent hyperlipemic syndromes in kittens should include the possibility of LPL deficiency as determined by PHPLA measurements.