青海省祁连圆柏天然林单木生物量模型构建

利用祁连圆柏整株生物量与生长指标数据，为估算祁连圆柏林的生物量估算提供参考。通过野外调查，共获取了63株祁连圆柏天然林样木生物量与生长指标实测数据。用其中50株样木数据进行回归模拟，用其余的13株样木数据对模型可靠性进行检验，构建器官生物量与生长指标间的回归模型。结果表明，祁连圆柏单木水平下，树干生物量模型的R²_adj为0.96，均方根、模型有效性和残差系数分别为0.50、0.85和0.05；枝条生物量模型的R²_adj为0.897，均方根、模型有效性和残差系数分别为0.69、0.80和-0.66；叶生物量模型的R²_adj为0.61，均方根、模型有效性和残差系数分别为0.54、0.86和0.15；根生物量模型的R²_adj为0.93，均方根、模型有效性和残差系数分别为0.12、0.997 和-0.01。在调查数据范围内构建的模型较好地反映了祁连圆柏生物量与生长指标间的关系，形式简单、使用方便；与实测值相比，树干与叶生物量模拟值偏小，枝和根偏大。     

Characterization of bovine ileal epithelial cell line for lectin binding,susceptibility to enteric pathogens,and TLR mediated immune responses

In this study, primary and immortalized bovine intestinal epithelial cells (BIECs) were characterized for the expression of surface carbohydrate moieties. Primary BIEC-c4 cells showed staining greater than 90 % for 16 lectins but less than 50 % staining for four lectins. Immortalized BIECs showed significantly different lectin binding profile for few lectins compared to BIEC-c4 cells. BIEC-c4 cells were studied for infectivity to E. coli, Salmonella enterica, bovine rotavirus, bovine coronavirus, and bovine viral diarrhea virus. Bovine strain E. coli B41 adhered to BIEC-c4 cells and Salmonella strains S. Dublin and S. Mbandaka showed strong cell invasion. BIEC-c4 cells were susceptible to bovine rotavirus. LPS stimulation upregulated IL-10, IL-8, and IL-6 expression and Poly I:C upregulated TLR 8 and TLR 9 expression. This study provides important knowledge on the glycoconjugate expression profile of primary and immortalized BIECs and infectivity and immune responses of primary BIECs to bacterial and viral pathogens or ligands.     

Replication of neurovirulent equine herpesvirus type 1 (EHV-1) in CD172a+ monocytic cells

Equine herpesvirus type 1 (EHV-1) is responsible for respiratory disorders, abortion and myeloencephalopathy (EHM) in horses. Two pathotypes of EHV-1 strains are circulating in the field: neurovirulent (N) and non-neurovirulent (NN). For both strains, CD172a⁺ monocytic cells are one of the main carrier cells of EHV-1 during primary infection, allowing the virus to invade the horse’s body. Recently, we showed that EHV-1 NN strains showed a restricted and delayed replication in CD172a⁺ cells. Here we characterize the in vitro replication kinetics of two EHV-1 N strains in CD172a⁺ cells and investigate if the replication of these strains is similarly silenced as shown for EHV-1 NN strains. We found that EHV-1 N replication was restricted to 7–8% in CD172a⁺ cells compared to 100% in control RK-13 cells. EHV-1 N replication was not delayed in CD172a⁺ cells but virus production was significant lower (10^3.0 TCID₅₀/10⁵ inoculated cells) than in RK-13 cells (10^8.5 TCID₅₀/10⁵ inoculated cells). Approximately 0.04% of CD172a⁺ cells produced and transmitted infectious EHV-1 to neighbour cells compared to 65% of RK-13 cells. Unlike what we observed for the NN strain, pretreatment of CD172a⁺ cells with histone deacetylases inhibitors (HDACi) did not influence the replication of EHV-1 N strains in these cells. Overall, these results show that the EHV-1 replication of N strains in CD172a⁺ cells differs from that observed for NN strains, which may contribute to their different pathogeneses in vivo.     

铯－134在幼龄茶树叶片中的分布特点

茶树上喷洒￣（１３４）Ｃｓ后，老叶上的沉积量和新叶上的吸收量均按指数回归形式下降，下降的速率以老叶上最快，春茶叶和夏茶叶上次之，秋茶叶上最慢；灌溉和土壤中拌入￣（１３４）Ｃｓ后，茶叶对￣（１３４）Ｃｓ的吸收分两个阶段进行，前期随着时间延长而增加。后期则减少。吸收量有个最大值；茶叶中￣（１３４）Ｃｓ的含量在喷洒情况下比灌溉和拌土的高。     

阔叶树种的研究现状与江西研究的发展方向

通过对阔叶树种在我国及江西省的研究状况的论述，初步总结了阔叶树种的研究水平及研究方向，指出了江西省阔叶树种研究存在的问题，探讨了江西省今后阔叶树种研究的方向。     

中日森林文化比较研究及启示

日本是一个森林大国，拥有着丰富的森林资源和森林文化，其教训和经验对中国乃至全世界的后工业化国家都有着很大的启发和借鉴意义。对日本的森林状况进行回顾和评价，从其森林思想中汲取精华，不断挖掘森林文化所蕴藏的丰富含义，恢复和弘扬森林文化，这对中国正在开展的生态文明建设以及解决环境问题都能提供思考和帮助。     

广东果树上17种拟茎点霉的RAPD分析

 自320个随机引物中筛选出适于拟茎点霉属真菌种间亲缘关系分析的15个随机引物,并优化了RAPD分析的扩增体系,在此基础上,对广东果树上17种拟茎点霉进行了RAPD分析。各菌株间的Nei相似系数UPGMA法聚类结果表明:来源于不同地区的2个Phomopsis mangiferae Ahmad菌株和2个P.macadami Z.D.Jiang et P.K.Chi菌株都分别以0.636和0.589的相似系数两两首先聚在一起,而不同的种则只在小于0.54的相似系数范围内聚类,体现了种间及种内的亲缘关系差异程度;聚类群与寄主植物不具相关性,同种植物上的不同拟茎点霉,即使是分自相同寄生部位也不能聚在一类;支持形态学上将生于柑桔枝和黄皮茎、沙梨叶和果、杨梅叶和枝以及同是生于龙眼叶的共8个拟茎点霉分别鉴定为不同的种,而不支持将P. cytosporella Penz.et Sacc.与P. mangiferae合并为一个种的观点;RAPD技术可作为拟茎点霉属真菌种间的亲缘关系分析的重要手段。     

铁在苹果树器官中的定量研究

成龄苹果树整株分析结果表明 ,果实、叶片、枝干、根系的鲜重分别占整株鲜重的 42 .0 9%、6 .33%、38.81%、12 .77% ,以果实占鲜重的比例最大 ,单株鲜重为 2 0 7.90kg ;果实、叶片、枝干、根系的干重分别占整株干重的 19.5 1%、6 .45 %、5 7.30 %、16 .75 % ,以枝干占干重的比例最大 ,单株干重 84.37kg ,单株含水量 12 3.5 5kg。铁浓度以直径 <0 .5cm的根最高 ,达 2 5 7.5 1mg/kg ,其次为叶片 ,达 2 14.0 9mg/kg ,果实中铁浓度最低 ,为 18.2 7mg/kg;单株总铁含量 4915 .78mg,叶、果、根、枝干中铁含量分别为 116 4.6 5、30 0 .72、15 0 0 .30、1949.6 0mg ,分别占单株总铁量的2 3 .70 %、6 .12 %、30 .5 2 %和 39.6 6 %。     

施肥对特高多花黑麦草初级生产力影响的研究

通过N、P、K肥单施及其不同配比混施，研究了施肥对提高特高多花黑麦草初级生产力的影响。结果表明，N、P、K肥单施和不同配比混施均能提高特高多花黑麦草的鲜草产量，但混施的增产效果比单施更好。施用尿素400kg／hm^2，过磷酸钙500kg／hm^2，硫酸钾200kg／hm^2，产量最高，播种后8周达到11723．3kg／hm^2。