绿色富硒生物猪饲料中AFB1达标生产工艺优化研究

饲料中黄曲霉毒素(AFB₁)容易超标,检出率达80%~100%,毒性大,具强致癌性,可抑制生猪免疫机能,降低动物生产性能,引起动物继发感染,还会在动物产品中残留而威胁人类健康,给生猪养殖带来经济损失,降低猪肉食品安全性。本文通过使用先进固体发酵系统设备和益生菌发酵技术,采用单因素试验和响应面中试优化,获得发酵降解猪饲料AFB₁的最佳工艺参数为硒浓度0.3 mg/kg,发酵时间12 h,量子波强度30 Hz,益生菌菌种组合CGMCC NO.17328混合CGMCC NO.15611。该工艺将猪饲料AFB₁量从63.41μg/kg降解到2.98μg/kg,降解率达到95.30%,AFB₁含量达到国家饲料安全标准。生产工艺适合养猪场低成本快速生产AFB₁达标猪饲料。     

内蒙古高原紫花苜蓿土壤氮素丰缺指标和推荐施氮量

为了给内蒙古高原紫花苜蓿（Medicago sativa L.）测土施氮奠定科学基础，本研究采用“零散实验数据整合法”和“养分平衡-地力差减法”新应用公式，开展了该自然区域紫花苜蓿土壤氮素丰缺指标和推荐施氮量研究。结果表明：内蒙古高原生长第1年紫花苜蓿土壤碱解氮第1~6级丰缺指标为≥48，20~48，8~20，4~8，2~4和<2 mg·kg^-1，土壤全氮第1~5级丰缺指标为≥1.4，0.8~1.4，0.4~0.8，0.2~0.4和<0.2 g·kg^-1，土壤有机质第1~6级丰缺指标为≥17，10~17，6~10，3~6，2~3和<2 g·kg^-1。当紫花苜蓿目标产量9~18 t·hm^-2、氮肥利用率40%时，内蒙古高原紫花苜蓿第1~6级土壤推荐施氮量分别为0，68~135，135~270，203~405，270~540和338~675 kg·hm^-2。     

水钾耦合对苜蓿根颈抗氧化特性及越冬率的影响

为探究不同水钾耦合处理对科尔沁沙地生境下种植苜蓿（Medicago sativa）抗寒生理特性的影响，以‘骑士T’和‘公农1号’苜蓿品种为试验材料，采用两因素裂区随机区组设计，水分处理为主区：灌水时间间隔分别为4 d，8 d和12 d（用W₁，W₂，W₃表示）；钾肥施用量水平为副区，具体施用量分别为50 kg·hm^-2 K₂O，100 kg·hm^-2 K₂O和150 kg·hm^-2K₂O（用K₁，K₂，K₃表示），并设置不施钾肥为对照（CK），于越冬前期挖取苜蓿根颈材料测定丙二醛（Malondialdehyde，MDA）、可溶性蛋白含量和抗氧化酶活性，翌年测定越冬率。结果表明：在W₃灌水时间间隔处理下，‘骑士T’和‘公农1号’品种苜蓿根颈的过氧化氢酶、过氧化物酶和超氧化物歧化酶活性、越冬率以及可溶性蛋白含量均明显高于W₁和W₂处理，其中以K₂施用量处理下的数值与CK差异最显著（P<0.05），且此施肥量下的MDA含量也显著低于CK（P<0.05）。因此在科尔沁沙地生境下生产苜蓿，灌水时间间隔为12 d、钾肥施用量在100 kg·hm^-2K₂O有利于提高苜蓿抗寒性。     

延迟收获对长江中游春玉米农艺性状及机收质量的影响

为探明长江中游玉米籽粒机械直收适宜品种与配套农艺措施，2018—2019年选用不同玉米品种，测定不同机收时间下玉米关键农艺性状、产量及机收质量指标。结果表明，收获时间对春玉米机收产量与机收质量均有显著影响。延迟1周收获后籽粒容重显著增加，机收产量显著提高，2年平均提高9.72%；而延迟2周收获则有降低机收产量的趋势。2年收获时杂质率总体≤3%，而机收籽粒破碎率与损失率均>5%，是该区域春玉米籽粒机收面临的主要问题。籽粒厚度、籽粒含水率和百粒重是影响机收籽粒破碎率的关键性状，三者与机收籽粒破碎率均呈显著的倒二次曲线关系；玉米的倒伏率、穗位高和重心高度是影响机收损失率的关键性状，倒伏率与机收损失率呈显著正相关，而穗位高和重心高度与机收损失率均呈显著的二次曲线关系。延迟收获能显著降低籽粒含水量，从而降低籽粒破碎率，但继续延迟收获有增加倒伏的风险。综上，长江中游春玉米成熟后适时延迟7~10 d收获，可有效降低籽粒含水量与机收籽粒破碎率，提高玉米籽粒机收产量。     

Leaf carbohydrates assimilation and metabolism affect seed yield of rapeseed with different waterlogging tolerance under the interactive effects of nitrogen and waterlogging

Waterlogging is a main factor causing rapeseed (Brassica napus L.) yield loss, and reasonable nitrogen (N) applications can compensate for this loss. To investigate the effects of N rates on seed yield of waterlogged rapeseed, the waterlogging-tolerant rapeseed variety ZS 9 and sensitive variety GH01 were waterlogged for 0 and 10 days with five leaves at the seedling stage under four N rates (0, 90, 180 and 270 kg/ha). Waterlogging significantly decreased seed yield, while N application can alleviate the yield loss. The yield decrease rate of waterlogged GH01 was greater than that of ZS 9 under the same N rate. During the seedling and bolting stage, the leaf photosynthetic rate (Pn) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity increased, while activities of adenosine diphosphate glucose pyrophosphorylase (AGPase), sucrose synthase (SuSy) and sucrose phosphate synthase (SPS) decreased with more N under the same watering conditions. Compared to the plants without waterlogging, the leaf Pn and Rubisco activity, starch and sucrose contents of waterlogged rapeseed decreased at the two stages; activities of AGPase, SuSy and SPS of waterlogged rapeseed decreased at the seedling while increased at the bolting stage for both the two varieties. At the flowering stage, the Pn, the activities of Rubisco, AGPase, SuSy, SPS and contents of sucrose, starch increased with more N application for both ZS 9 and GH01. Compared to the plants without waterlogging, the Pn and Rubisco activity for waterlogged plants of the two varieties increased; the waterlogged plants of tolerant variety had higher activities of AGPase, SuSy and SPS, while those of sensitive variety was significantly lower. However, the decreased starch and sucrose content were found in both tolerant and sensitive varieties. The activities of AGPase, SuSy and SPS at flowering were highly positively correlated with yield under the interactive effects of N and waterlogging. These results suggested that the flowering stage is the most important stage that N had the positive regulation on waterlogged rapeseed growth. The carbohydrates translocation from leaves to seeds of the tolerant variety were enhanced after waterlogging, while that of the sensitive variety was still inhibited. This was the main reason for the difference in yield between the two waterlogged varieties.     

三种草坪草净光合速率和蒸腾速率的日变化特点研究

研究了在晴天的条件下，假俭草、细叶结缕草和地毯草净光合速率和蒸腾整编的日变化特点。结果表明：（1）假俭草和细叶绍缕草的净光合速率的日变化曲线均为双峰型，而地毯草为三峰型，3种草出现峰值的时间不同，均有明显的“午睡”现象，日平均净光全速率的大小顺序为俭草＞地毯草＞细叶结缕草；（2）假俭草和细叶结缕草的蒸腾速率的日变化曲线均为双峰型,而地毯草为单峰型，3种草出现峰值的时间有所判差别，“午睡”现象均不明显，日平均蒸腾速率的大小顺序为细叶绍缕草＞地毯草＞假俭草；（3）不同生态因子与3种草坪草净光合速率、蒸腾速率的相关程度均不相等。同种态因子与不同草坪草的净光合速率、蒸腾速率的相关程度也不一样。     

THE EFFECT OF A COMBINATION OF MEDETOMIDINE-BUTORPHANOL AND MEDETOMIDINE, BUTORPHANOL, ATROPINE ON GLOMERULAR FILTRATION RATE IN DOGS

The effects of intramuscularly administered medetomidine and butorphanol (MB), and medetomidine, butorphanol, atropine (MBA) on glomerular filtration rate (GFR) were determined in six dogs as measured by 99m-Tc-labeled diethylenetriaminepentaacetic acid (99mTc-DTPA) nuclear scintigraphy. Direct systolic, diastolic, and mean arterial blood pressures and heart rate were measured at regular time intervals before, during, and after GFR calculations. The mean GFR measurement following MB was significantly greater (4.44 ml/min/kg) than following MBA (3.82 ml/min/kg) or saline treatment (3.41 ml/min/kg). There was no significant difference between the mean GFR measurements following MBA injection and following saline injection. Diastolic and mean arterial pressures following MBA injection were significantly higher than the values recorded after either MB or saline alone. Heart rate following MB administration was significantly lower than that recorded for dogs receiving MBA or saline alone. The results of this study indicate that the administration of medetomidine in combination with butorphanol significantly increases total GFR in healthy dogs, while the administration of the combination of medetomidine, butorphanol, and atropine does not.     

Effects of predator-induced visual and olfactory cues on 0+ perch (Perca fluviatilis L.) foraging behaviour

Abstract –  Foraging juvenile fish with relatively high food demands are usually vulnerable to various aquatic and avian predators. To compromise between foraging and antipredator activity, they need exact and reliable information about current predation risk. Among direct predator-induced cues, visual and olfactory signals are considered to be most important. Food intake rates and prey-size selectivity of laboratory-reared, naive young-of-the-year (YOY) perch, Perca fluviatilis , were studied in experiments with Daphnia magna of two size classes: 2.8 and 1.3 mm as prey and northern pike, Esox lucius , as predator. Neither total intake rate nor prey-size selectivity was modified by predator kairomones alone (water from an aquarium with a pike was pumped into the test aquaria) under daylight conditions. Visual presentation of pike reduced total food intake by perch. This effect was significantly more pronounced (synergistic) when visual and olfactory cues were presented simultaneously to foraging perch. Moreover, the combination of cues caused a significant shift in prey-size selection, expressed as a reduced proportion of large prey in the diet. Our observations demonstrate that predator-induced olfactory cues alone are less important modifiers of the feeding behaviour of naive YOY perch than visual cues under daylight conditions. However, pike odour acts as a modulatory stimulus enhancing the effects of visual cues, which trigger an innate response in perch.     

利用高等数学推导边际分析在经济学中的应用

本文从高等数学的基本理论导数的概念出发，引出了经济管理学中重要的概念边际函数，通过介绍经济科学中常用的函数及大量的实例，探讨了高等数学在经济管理学中的应用，给出了解决这些问题的一般方法。     

Study on Site-specific Nutrient Management in Cotton Field

The study on the characteristics of spatial variability of soil nutrients and fertilizer recommendations in cotton field under certain conditions of agricultural management was conducted with GIS and systematic approach for soil nutrient constrains. The results showed that of the spatial variability of soil nutrient was greatly related to the management condition of previous crops. Grid sampling and variable rate application technology (VRAT) were the tools that would hopefully increase fertilizer efficiency. The fertilizers were applied where they were needed and at proper rate. Balance fertilization demonstration showed that fertilizer recommendations according to the available nutrient level in soil could decrease fertilizer cost with 657.4 yuan / ha and increase seed cotton yield by 19.8%. A net profit of the balanced fertilization was 5314.9 yuan / ha higher than that of local fertilization practice.