首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   1691篇
  免费   123篇
  国内免费   122篇
林业   165篇
农学   124篇
基础科学   108篇
  178篇
综合类   820篇
农作物   48篇
水产渔业   77篇
畜牧兽医   344篇
园艺   14篇
植物保护   58篇
  2024年   11篇
  2023年   26篇
  2022年   45篇
  2021年   64篇
  2020年   61篇
  2019年   83篇
  2018年   44篇
  2017年   67篇
  2016年   70篇
  2015年   60篇
  2014年   99篇
  2013年   122篇
  2012年   134篇
  2011年   152篇
  2010年   140篇
  2009年   116篇
  2008年   98篇
  2007年   86篇
  2006年   80篇
  2005年   76篇
  2004年   51篇
  2003年   42篇
  2002年   26篇
  2001年   26篇
  2000年   22篇
  1999年   20篇
  1998年   14篇
  1997年   22篇
  1996年   9篇
  1995年   12篇
  1994年   10篇
  1993年   7篇
  1992年   9篇
  1991年   6篇
  1990年   8篇
  1989年   5篇
  1988年   3篇
  1987年   3篇
  1986年   2篇
  1983年   1篇
  1982年   1篇
  1981年   1篇
  1980年   2篇
排序方式: 共有1936条查询结果,搜索用时 15 毫秒
1.
书报编排的标准化和规范化,有利于书报所载信息的正确传递,存贮以至国际化。做好书报编排的标准化和规范化工作是时代赋予编辑工作者的职责。本文就我国目前书报编排中存在的主要问题人为6类,并作分析讨论。  相似文献   
2.
本文运用水流相似原理,对可控止回阀的流阻系数曲线和动水力矩,进行了系统的水力模型试验研究,为该阀的节能效益和技术开发的可行性,提供了必要的科学依据,其流阻系数值为0.19,优于液控蝶阀。  相似文献   
3.
A semi-energy method to analyse the buckling behaviour of edge-stiffened plates under combined compression and bending is presented in this paper, One unloaded edge of the plate is restrained elastically against rotation and the other is stiffened by a st  相似文献   
4.
The induction of a hypersensitive reaction in Samsun NN tobacco by tobacco mosaic virus (TMV) at 20°C leads to the development of both localized and systemic acquired resistance, and is associated with the appearance of pathogenesis-related proteins (PR's) and large increases in peroxidase activity and ethylene production. Salicylic acid (SA) induced a similar resistance in treated plant parts and occasionally also in untreated upper leaves of plants of which three lower leaves had been injected. SA also induced the same four PR's, but these were confined to the treated leaves. Thus, the connection between the presence of PR's and the reduction of TMV multiplication and spread may not be direct.In contrast to TMV, SA did not stimulate ethylene production and hardly increased peroxidase activity. Induction of acquired resistance and PR's by SA developed equally well at 20°C and at 32°C. However, pricking leaves with needles moistened with the ethylene-releasing compound ethephon mimicked TMV infection in inducing acquired resistance and PR's in both treated and untreated leaves at 20°C, but not at 32°C. Ethephon increased peroxidase activity at both temperatures, but only at 20°C dit it induce changes in both the anodic and the cathodic isoenzymes that were similar to those induced as a result of TMV infection. SA induced PR's and reduced TMV multiplication in Samsun tobacco, and inhibited virus spread in Samsun NN at 32°C.These observation indicate that neither the induction of PR's, nor the development of acquired resistance is temperature-sensitive. On the other hand, the effects of ethephon are temperature-sensitive in the same way as the hypersensitive response to TMV. It can thus be hypothesized that ethylene, produced naturally during the hypersensitive reaction of tobacco to TMV, leads to the temperature-sensitive synthesis or release of a presumably benzoic acid-type compound that functions as the natural inducer of PR's and acquired resistance. Although vanillic acid has been shown to accumulate in hypersensitively reacting tobacco leaves, it produced none of the effects of SA, and thus cannot be the natural inducer.Samenvatting Inductie van een overgevoeligheidsreactie in Samsun NN-tabak door tabaksmozaïekvirus (TMV) bij 20°C leidt tot de ontwikkeling van een verworven resistentie die zowel lokaal als systemisch werkzaam is, en gaat samen met het verschijnen van pathogenesis-related proteins (PR's) en sterke toename in de activitieit van peroxidase en de produktie van ethyleen. Salicylzuur (SA) induceerde een vergelijkbare resistentie in behandelde plantedelen en af en toe ook in niet behandelde bovenbladeren van planten waarvan drie onderbladeren waren ingespoten. SA induceerde ook dezelfde vier PR's, maar deze waren beperkt tot de behandelde bladeren. Er bestaat dus geen directe samenhang tussen de aanwezigheid van PR's en de remming van de vermeerdering en uitbreiding van TMV in de plant.In tegenstelling tot TMV stimuleerde SA de ethyleenproduktie niet en verhoogde het de peroxidaseactiviteit nauwelijks. Inductie van verworven resistentie en PR's door SA trad even goed op bij 32°C als bij 20°C. Net als infectie met TMV leidde aanprikken van bladeren met naalden die gedoopt waren in een oplossing van ethefon — waaruit in het blad ethyleen vrijkomt — echter tot inductie van verworven resistentie en PR's in zowel behandelde als onbehandelde bladeren bij 20°C, maar niet bij 32°C. Ethefon verhoogde de peroxidaseactiviteit bij beide temperaturen, maar alleen bij 20°C induceerde het veranderingen in zowel de anodische als de kathodische isoënzymen die vergelijkbaar waren met die welke geïnduceerd werden als gevolg van infectie met TMV. SA induceerde PR's en verminderde de vermenigvuldiging van TMV in Samsun tabak, en remde de uitbreiding van het virus in Samsun NN bij 32°C.Deze waarnemingen tonen dat noch de inductie van PR's, noch de ontwikkeling van verworven resistentie een temperatuurgevoelig proces is. Daarentegen zijn de effecten van ethefon op dezelfde wijze temperatuurgevoelig als de overgevoeligheidsreactie op TMV. Men kan daarom veronderstellen dat ethyleen, dat op natuurlijke wijze geproduceerd wordt tijdens de overgevoeligheidsreactie van tabak op TMV, aanleiding geeft tot een temperatuurgevoelig proces, namelijk de synthese of het vrijkomen van een verbinding, vermoedelijk een benzoëzuurderivaat, dat fungeert als de natuurlijke inductor van PR's en verworven resistentie. Hoewel is aangetoond dat vanillinezuur zich ophoopt in overgevoelig reagerende tabaksbladeren, veroorzaakte deze verbinding geen enkel van de effecten van SA. Vanillinezuur kan dus niet de natuurlijke inductor zijn.  相似文献   
5.
作者采用室内外结合的方法,根据台湾稻螟幼虫、蛹、卵的形态特征,制定了台湾稻螟分龄分级标准,并按所测定的相应虫态历期,采用分龄分级法进行了3年的发生期预测验证,平均准确率达96.7%,用于指导大田防治1.93万hm~2,平均防治效果达85.8%。  相似文献   
6.
隐性白羽肉鸡及其配套地方鸡部分血液生化指标的测定   总被引:1,自引:1,他引:1  
本试验测定了我校最新育成的隐性白羽肉鸡快慢羽系父系、母系及其配套地方鸡广西黄鸡和文昌鸡主要的血液生化指标。结果表明,四个品系血清总蛋白、白蛋白、葡萄糖、胆固醇、Ca含量接近,血清P、K、Na含量A系最高,显著(P<0.05)或极显著(P<0.01)高于其它三系,各品系内公母之间母鸡的血Ca均极显著(P<0.01)高于公鸡。血清淀粉酶(AMY)活力和肌酸磷酸激酶(CK)活力B系极显著(P<0.01)高于其它三系,血清碱性磷酸酶(AKP)活力品系间差异不显著(P>0.05)。  相似文献   
7.
用商品益生素和自制益生素分别灌服1日龄雏鸡,灌服益生素后1、4、7、10、18d测定胸腺、脾脏、法氏囊的器官指数动态变化,用间接酶联免疫吸附试验(ELISA)方法测定泪液、气管液、胆汁和肠液的IgA、IgM、IgG相对含量的动态变化,结果发现服用商品益生素的雏鸡免疫器官指数在第7天高于未服用益生素的对照雏鸡,上述4种体液的IgA、IgM、IgG相对含量在服用益生素后7~10d高于未服用益生素的对照雏鸡,服用自制益生素的雏鸡上述4种体液的IgA、IgM、IgG相对含量在服用益生素后4~7d高于未服用益生素的对照雏鸡。表明益生素对雏鸡的免疫系统有一定的影响,能够促进免疫器官的生长发育,提高雏鸡呼吸道和消化道局部体液的免疫球蛋白相对含量。  相似文献   
8.
ABSTRACT

The present study was conducted to estimate milk production performance and fit lactation curves for groups of ewes of Local and of Awassi crosses, with a variable blood level, reared under farmer’s environment. The Weigh-Suckle-Weigh method plus hand milking was used to estimate milk yield for ewes. A total of 466 observations from 115 ewes were used. Estimated least-squares adjusted means for the milk production over 120 days were 0.56?kg day?1 (Local), 0.67 (<30% Awassi), 0.86 (30–50% Awassi), and 0.96 (>50% Awassi). Groups with 30–50% Awassi and >50% Awassi ewes produced significantly (p?<?0.05) more milk than Local ewes. Significant differences were observed between <30% Awassi and >50% Awassi crossbred groups. The best crosses (>50% Awassi) produced over 70% more milk than the local ewes which demonstrates the potential that exists in increasing milk production through the initiated crossbreeding programme with sheep in Ethiopia.  相似文献   
9.

Objectives

To describe colostrum management practices carried out in northern Victorian dairy herds and to identify weaknesses in these areas that may affect calf health and welfare by comparing the results with the current industry recommendations

Methods

A questionnaire to obtain information about colostrum management and calf‐rearing practices was sent to commercial dairy farming clients of Rochester Veterinary Practice between June and September 2013. The questionnaire consisted of a general herd overview and colostrum harvesting practices.

Results

The response rate was 39% (58/150). Many dairy producers were not meeting the current industry recommendations in the following areas: (1) time of removal calf from the dam, (2) relying on calf suckling colostrum from the dam to achieve adequate passive transfer, (3) failing to supplement calves with colostrum, (4) feeding inadequate volumes of colostrum, (5) delayed colostrum harvesting, (6) pooling of colostrum, (7) failing to objectively assess colostrum quality or relying on visual assessment and (8) storing colostrum for a prolonged periods of time at ambient temperatures.

Conclusion

The results from this survey highlight the need for greater awareness of industry standards for colostrum management and feeding hygiene.
  相似文献   
10.
AIM: To develop and validate a simple and sensitive method using liquid chromatography-mass spectrometry (LC-MS) for quantification of articaine, and its major metabolite articainic acid, in plasma of red deer (Cervus elaphus), and to investigate the pharmacokinetics of articaine hydrochloride and articainic acid in red deer following S/C administration of articaine hydrochloride as a complete ring block around the antler pedicle.

METHODS: The LC-MS method was validated by determining linearity, sensitivity, recovery, carry-over and repeatability. Articaine hydrochloride (40?mg/mL) was administered S/C to six healthy male red deer, at a dose of 1?mL/cm of pedicle circumference, as a complete ring block around the base of each antler. Blood samples were collected at various times over the following 12 hours. Concentrations in plasma of articaine and articainic acid were quantified using the validated LC-MS method. Pharmacokinetic parameters of articaine and articainic acid were estimated using non-compartmental analysis.

RESULTS: Calibration curves were linear for both articaine and articainic acid. The limits of quantifications for articaine and articainic acid were 5 and 10?ng/mL, respectively. Extraction recoveries were >72% for articaine and >68% for articainic acid. After S/C administration as a ring block around the base of each antler, mean maximum concentrations in plasma (Cmax) of articaine were 1,013.9 (SD 510.1) ng/mL, detected at 0.17 (SD 0.00) hours, and the Cmax for articainic acid was 762.6 (SD 95.4) ng/mL at 0.50 (SD 0.00) hours. The elimination half-lives of articaine hydrochloride and articainic acid were 1.12 (SD 0.17) and 0.90 (SD 0.07) hours, respectively.

CONCLUSIONS AND CLINICAL RELEVANCE: The LC-MS method used for the quantification of articaine and its metabolite articainic acid in the plasma of red deer was simple, accurate and sensitive. Articaine hydrochloride was rapidly absorbed, hydrolysed to its inactive metabolite articainic acid, and eliminated following S/C administration as a ring block in red deer. These favourable pharmacokinetic properties suggest that articaine hydrochloride should be tested for efficacy as a local anaesthetic in red deer for removal of velvet antlers. Further studies to evaluate the safety and residues of articaine hydrochloride and articainic acid are required before articaine can be recommended for use as a local anaesthetic for this purpose.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号