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Abstract. Recent developments in in situγ ray spectrometry offer a new approach to measuring the activity of radionuclides such as 137Cs and 40K in soils, and thus estimating erosion or deposition rates and field moist bulk density (ρm). Such estimates would be rapid and involve minimal site disturbance, especially important where archaeological remains are present. This paper presents the results of a pilot investigation of an eroded field in Scotland in which a portable hyper pure germanium (HPGe) detector was used to measure γ ray spectra in situ. The gamma (γ) photon flux observed at the soil surface is a function of the 137Cs inventory, its depth distribution characteristics and ρm. A coefficient, QCs, derived from the forward scattering of 137Cs γ ray photons within the soil profile relative to the 137Cs full energy peak (662 keV), was used to correct the in situ calibration for changes in the 137Cs vertical distribution in the ploughed field, a function of tillage, soil accumulation and ρm. Based on only 8 measurements, the agreement between in situγ ray spectrometry and soil sample measurements of 137Cs inventories improved from a non significant r2=0.05 to a significant r2=0.62 (P<0.05). Erosion and deposition rates calculated from the corrected in situ137Cs measurements had a similarly good agreement with those calculated from soil cores. Mean soil bulk density was also calculated using a separate coefficient, QK, derived from the forward scattering γ photons from 40K within the soil relative to the 40K full energy peak (1460 keV). Again there was good agreement with soil core measurements (r2=0.64; P<0.05). The precision of the in situ137Cs measurement was limited by the precision with which QCs can be estimated, a function of the low 137Cs deposition levels associated with the weapons testing fallout and relatively low detector efficiency (35%). In contrast, the precision of the in situ ρm determination was only limited by the spatial variability associated with soil sampling.  相似文献   
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Modern biotechnology promises a number of new applications in animal breeding and production. Although conventional pig breeding has achieved a high level of efficiency and productivity numerous problems have been encountered with animal health and the loss of meat quality. Selection based on phenotypic performance data of individual animals does not take into account the importance of specific genes and their relevance within a complex regulatory system. In most cases it is therefore difficult to trace back the genetic origins of clinically important disorders. The application of genetic engineering techniques in pig production will facilitate diagnosis, improvement of productivity, and animal health by allowing direct genetic manipulation. Attention must be focussed on the physical and genetic analysis of the procine genome. The isolation and characterisation of genes, DNA-markers, polymorphic DNA-fragments, and their chromosomal assignment will be important prerequisites and tools for the elucidation of genetic disorders. Especially the detection of heterozygous carriers of recessive disorders and their elimination from the breeding stock will increase selection accuracy and decrease the generation intervals. But also the rapid and simple detection of infectious diseases, which is sometimes difficult if not impossible at present, will improve animal health and welfare. Although the production of transgenic animals either by DNA-microinjection into zygotes or the use of embryonal stem cells manipulated in vitro is less straightforward than DNA-based diagnosis it will play an important role in the direct manipulation of the porcine genome and genes. Breeding programmes including the use of transgenic livestock have already been developed. There is no doubt that genetic engineering has reached a degree of practical feasibility, allowing it to play an important role in pig breeding in particular and animal production in general.  相似文献   
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本文对天山云杉人工幼林树冠结构以及树冠结构与林木之间的关系进行了研究,并基于生物学原理,在确定有效树冠的法础上,通过决定林木能力的树冠特征对其人林分间伐中的应用进行了初步研究。  相似文献   
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Introgression of trichome-mediated insect resistance from the wild speciesSolanum berthaultii has become a major focus of the potato improvement program at Cornell University during the past twelve years. Several quantitative characters are involved in this resistance which is effective against a wide range of pest types. Correlative biochemical assays have been developed to assay specific components of the resistance, and the effects of the resistance on the target pests have been studied. Quantitative laboratory assays and specific measurements of insect behavior and biology have increased the precision of selection and enable the investigation of the genetic control of the resistance.We are currently using restriction fragment length polymorphisms (RFLPs) for genetic mapping of factors controlling the trichome traits fromS. berthaultii. Backcrosses to both the wild and the cultivated species parents have been evaluated for phenotypes contributing to the resistance mechanism, including trichome density, sucrose ester and polyphenol oxidase production by the trichomes, and the enzymatic browning reaction responsible for insect entrapment. Genetic maps are being developed for these progenies, using RFLP markers previously mapped in potato. Field and greenhouse trials under insect infestations are also being conducted with the mapping progeny. Our goal is to locate genes responsible for quantitative insect resistance by correlating RFLP variation at mapped loci with the trichome phenotypes and insect resistance. Genetic markers for these traits will be useful in transfer of the effective wild chromosomal segments into and among tetraploid potatoes, and for a better understanding of the resistance mechanism.  相似文献   
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为挖掘更多的茎基腐病抗性QTL用于分子标记辅助育种,以中抗茎基腐病品种CI12633和感茎基腐病品种扬麦158的重组自交系群体为材料,采用SSR和SNP等分子标记对群体中的94个家系进行基因型分析,绘制遗传连锁图,并结合3次室内群体的茎基腐病抗性鉴定结果对小麦茎基腐病抗性QTL进行定位.结果表明,与小麦茎基腐病抗性相关...  相似文献   
8.
本研究旨在探究产胞外多糖芽孢杆菌对蔬菜镉(Cd)积累及土壤结构的影响。采用以空心菜和茼蒿为供试材料的盆栽试验,研究了功能菌株Priestia megaterium YG35和Bacillus halodurans G20降低蔬菜吸收Cd的效果和可能作用机制。结果表明,与CK相比,菌株YG35、菌株G20及其胞外多糖处理显著增加空心菜和茼蒿可食用组织干质量(37.8%~115.1%),显著降低可食用组织Cd含量(21.9%~44.2%),并使茼蒿Cd含量(0.057~0.061 mg·kg-1)达到安全可食用标准,显著降低根际土壤有效态Cd含量(3.7%~11.7%),显著(P<0.05)增加根际土壤多糖含量(35.9%~49.5%)和蔗糖酶活性(15.3%~28.4%),促进根际土壤团聚体向大粒径转化。研究表明,YG35和G20及其胞外多糖能降低蔬菜对Cd的吸收,促进蔬菜生长,改善土壤结构,具有在Cd污染土壤上实现蔬菜安全生产的应用潜力。  相似文献   
9.
CH7034是一个兼抗小麦白粉病和条锈病的新种质材料,通过普通小麦与八倍体小偃麦"小偃7430"杂交、回交选育而成.为明确其白粉病抗性的遗传机制及抗性基因的染色体位置,用小麦高感品系"SY95-71"与CH7034杂交,所获F1、F2及其双亲在温室用白粉病E09菌系的15号小种接种,对CH7034的白粉病抗性进行鉴定和遗传分析.结果表明,无论是苗期还是成株期,CH7034对白粉病菌均表现为免疫,且具有与其抗性供体小偃7430及野生亲本长穗偃麦草相似的白粉病抗性,F1代抗病反应型为O或O'级,F2代抗感分离比符合R:S=3:1,说明CH7034抗性受显性单基因控制.用307对小麦微卫星引物对一个148株的F2群体进行分析,发现小麦微卫星标记xgwm311与抗病基因连锁,遗传距离为12.4 cM.用中国春缺-四体和双端体材料进一步验证与抗病相关的片段位于2A染色体的长臂上,进而将CH7034所含的抗白粉病基因定位于小麦的2AL上.  相似文献   
10.
Rapeseed(Brassica napus L.) is the world?s second produced oilseed and accounts for nearly 12% of world major vegetable oil production. For the last 10 years, the production, planting area, and yield of rapeseed have been stable, with improvement of seed quality and especially seed oil content. This paper presented the worldwide rapeseed research by using the bibliometric analysis of papers published during the period 2011–2021. In total,7617 articles and reviews were included in this analysis. ...  相似文献   
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