饲料中无机元素分析方法研究进展

饲料中所含的无机元素对动物生长具有重要意义。在饲料中过量添加无机元素会导致环境污染问题,也会通过食物链最终危害消费者的身体健康,因此,对饲料产品中的无机元素进行检测和动态监控尤为重要。综述了近年来饲料中无机元素含量检测主要应用的前处理方法及其含量检测技术,分析了不同前处理方法和含量检测技术的优点和不足,以期为饲料中无机元素分析方法的科学、合理应用提供参考。     

Ovarian mixed germ-cell tumor comprising mature teratoma and embryonal carcinoma in a four-toed hedgehog (Atelerix albiventris)

This report describes the clinical and histopathological characteristics of a rare mixed germ-cell tumor comprising teratoma and embryonal carcinoma in the left ovary of a 10-month-old four-toed hedgehog, with chief complaints of loss of appetite and lethargy. Laparotomy revealed a swollen left ovary with small disseminated peritoneal nodules, and bilateral ovariohysterectomy was performed. The left ovary had a mature teratoma with well-differentiated fat, bone, cartilage, salivary gland, trachea, keratin cyst, and nervous tissues, and an embryonal carcinoma consisting of poorly-differentiated epithelial cells arranged in tubular, alveolar, or solid patterns. Immunohistochemically, the embryonal carcinoma cells were positive for placental alkaline phosphatase and c-KIT. This is the first case of mature teratoma with embryonal carcinoma in the ovary of a hedgehog.     

酸与碱处理对海带多糖提取及其抗氧化活性的影响

本试验采用柠檬酸提取法和碱提取法从海带中提取多糖组分,以总抗氧化能力和羟自由基（·OH）为衡量抗氧化活性指标,比较这两种提取方法得到海带粗多糖产率和抗氧化活性。试验结果表明：柠檬酸提取法和碱提取法的提取率分别为15.92%和6.05%,所提取的海带粗多糖具有良好的抗氧化活性,且柠檬酸提取法抗氧化活性更高。 [关键词] 柠檬酸提取法|碱提取法|海带多糖|抗氧化活性     

孔雀石绿胶体金检测样品前处理工艺的优化

为建立快速筛选孔雀石绿的前处理方法,对标行标水产品中孔雀石绿的快速检测胶体金免疫层析法（KJ201701）,用于水产品中药物残留的现场快速检测使用。试验采用提取、氧化、浓缩和复溶四个步骤,对氮吹、SPE柱、反萃取三种不同提取方式进行前处理优化和比较,通过胶体金免疫层析试纸条进行检测。结果表明：三种方法均能达到同时检测水产品中孔雀石绿的目的,检测时间为反向萃取法相似文献   

Study on Modeling for Pretreatment and Cyclic Control Stream

The characteristics of pretreatment and cyclic control stream are analysed:the form of cyclic embedded hardware circuit,the idea for designing the pretreatment program.The chart model of the system is established preliminarily.     

碱法草浆造纸蒸煮黑液厌氧生物降解及其主要微生物类群研究

采用中温(35±1℃)UASB AF相结合的反应器处理碱法草浆造纸蒸煮黑液,设计了两种试验方案。试验方案Ⅰ-碱法草浆黑液酸化后进行厌氧发酵,Ⅰ号反应器内装陶粒。试验方案Ⅱ-黑液用化学法(HCl)去除木质素后进行厌氧发酵,Ⅱ号反应器内装软填料。进行HRT、有机负荷和有机污染物(COD_(cr)、BOD_5)去除效率、沼气产气率相关性试验。在厌氧发酵稳态运行条件下,采用Hungate厌氧操作技术,MPN计数法对UASB AF反应器内厌氧污泥中的主要微生物类群区系组成进行了生态分析研究。     

Study on Soil Improvement Measure of Plant Landscape Construction in Saline and Alkaline Area in Tianjin

Tianjin is located in North China, which is semi- arid climate, next to east of Bohai, but being influ- enced little by internal bay. The urban district and strand area locates in a crossing area between the al- luvial plain of Hai River and the land form…     

芒果中性/碱性转化酶MiNI基因的克隆与表达分析

采用RT-PCR技术克隆了1个编码NI基因的cDNA序列,命名为MiNI基因,并对不同来源的中性/碱性转化酶的分子特征及系统进化进行比较分析。结果表明：MiNI基因开放阅读框为2034 bp,编码677个氨基酸,相对分子量为76.6 kDa,理论等电点为6.24;生物信息学分析结果显示,NI二级结构α螺旋占38.85%,无规则卷曲占35.45%,伸展链占18.91%,β折叠占6.79%;MiNI具有glycoside hydrolase family 100结构保守域,与克里曼丁桔、龙眼、巴西橡胶树和番木瓜都具有一致的motif位点;MiNI基因编码的氨基酸序列与克里曼丁桔、龙眼氨基酸序列同源性最高;构建NI系统进化树分析表明,与芒果遗传距离最近的是克里曼丁桔,最远的是玉米和枸杞。qRT-PCR分析显示,果皮MiNI基因表达量远高于果肉;花后10~40 d,果皮MiNI基因表达量显著下降;花后40~100 d,果皮MiNI基因表达量维持在一个相对稳定水平;花后100~130 d,随着果实成熟,果皮MiNI基因表达量又显著上升;而花后10~40 d,果肉MiNI基因表达量显著下降,至果实发育后期果肉MiNI基因表达量始终处于极低水平。该研究为进一步了解MiNI基因在芒果果实蔗糖代谢过程中的作用以及从分子角度阐明芒果糖代谢机理奠定理论和技术基础。