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1.
The survival of Leptosphaeria maculans , which causes phoma stem canker (blackleg), on oilseed rape residues ( Brassica napus ) in South Australia was investigated. Using a quantitative polymerase chain reaction (PCR) assay for L. maculans DNA, the pathogen was mainly detected in the upper 5 cm of the soil profile, including residues on the soil surface. As the size of organic matter particles in the soil decreased, so did the quantity of L. maculans detected in them. To obtain representative data for a field, at least 30 subsamples needed to be collected over the 0·81 ha area studied. In a survey of 49 commercial fields in South Australia, most L. maculans was detected in fields 1 year after oilseed rape had been grown, with less detected after 2 years and negligible amounts 3 years or more after cropping. The diagnostic DNA-based assay for L. maculans reduced the time and cost of studying L. maculans survival in soil and increased the sensitivity and accuracy of results compared with estimates of propagule number of colony-forming units on a semiselective medium. 相似文献
2.
试验于1992~1994年在山东和北京的6个地区,14个苹果园进行。田间试验表明,用灭菌肥Ⅰ型50倍液涂治苹果树腐烂病患处,平均治愈率可达90%以上,比对照2%福美砷当年平均治愈率提高7.22─29.51%,周年平均治愈率提高18.05%~32.39%,愈伤组织宽度增加3.03%~29.23%。室内试验表明,灭菌肥Ⅰ型最低抑菌剂量1000倍,抑菌面积可达2289.06mm2。 相似文献
3.
T. W. Hofman P. H. J. Jongebloed 《European journal of plant pathology / European Foundation for Plant Pathology》1988,94(5):243-252
The infection process ofRhizoctonia solani AG-3 was studied on potato sprouts, cv. Bintje, in growth chamber trials at 15 °C. Initially hyphae ofR. solani grew predominantly in the longitudinal direction of the sprouts (runner hyphae). They tended to follow the junctions between epidermis cells as was observed by SEM. The hyphae formed side-branches mainly half-way of the subterranean parts of the sprouts. They branched several times with short swollen cells to form infection cushions. Lesions developed only underneath the infection cushions and were first observed five days after inoculation. The necrotic area was proportional to the area covered with infection cushions on the sprouts. Depth of the lesions could extend up to the vascular bundle. Sprouts were colonized only in healthy tissue in the epidermal layer underneath the infection cushion and in necrotic tissue. A few days after appearance of the lesions,R. solani formed brown, uninfective mycelium on and in the circumference of these lesions.Aldicarb did not influence any part of the infection process. Ethoprophos delayed the emergence of sprouts, but increased the number of sprouts per tuber. As soon as sprouts had emerged, growth was considerably promoted by ethoprophos. Ethoprophos delayed the appearance of lesions and reduced their size. Oxamyl showed the same effects to a smaller extent.As the size of lesions appears to be proportional to the size of the infection cushions, any agents that change the size of the infection cushions, such as pesticides or antagonists, may alter the severity of the disease.Samenvatting Het infectieproces vanRhizoctonia solani AG-3 werd bestudeerd op aardappelspruiten, cv. Bintje, in een klimaatcel bij 15C. Aanvankelijk groeide de schimmel met runnerhyfen voornamelijk in de lengterichting van de spruit. Via SEM kon waargenomen worden, dat de hyfen hierbij vooral over de begrenzingen van de epidermiscellen groeiden. Het mycelium vormde veel zijvertakkingen, bestaande uit iets gezwollen korte cellen, welke voornamelijk halverwege op het ondergrondse deel van de spruit gevormd werden. Een dichte massa van deze cellen vormde een infectiekussentje. Lesies, welke vanaf vijf dagen na inoculatie werden waargenomen, bevonden zich slechts onder spruitoppervlak bezet met infectiekussentjes. De lesiegrootte was recht evenredig met het spruitoppervlak dat bezet was met infectiekussentjes. De diepte van de lesies reikte tot aan de vaatbundels. De spruit werd alleen door de schimmel gekoloniseerd in gezond epidermisweefsel onder het infectiekussentje en in necrotisch weefsel. Enkele dagen na verschijning van lesies vormde R.solani bruin, niet infectieus, mycelium op en rondom de lesies.Aldicarb had geen effect op het infectieproces. Ethoprophos vertraagde de opkomst en verhoogde het aantal tot ontwikkeling gekomen spruiten per knol in gestoomd zand. Direct na opkomst had ethoprophos echter een sterk groeistimulerend effect. Ethoprophos vertraagde de lesievorming en reduceerde de lesiegrootte, vergeleken met onbehandelde planten. Oxamyl vertoonde deze effecten in geringere mate.Daar de lesiegrootte direct gecorreleerd blijkt met de grootte van het infectiekussentje, mag verwacht worden dat elke beïnvloeding van de ontwikkeling van het mycelium van R.solani, bijvoorbeeld door pesticiden of antagonisten, een verandering van de lesiegrootte ten gevolge heeft. 相似文献
4.
核桃早实性状的RAPD分析 总被引:18,自引:0,他引:18
用BSA (Bulked Segregant Analysis) 法获得了与核桃早实性状相关的RAPD 标记OPB208900。混合分离群体的混合样来自早实品种辽1 的自然授粉实生后代。用220 个随机引物扩增早实和晚实混合样, 只有引物OPB208 ( 5. GTCCACACGG 3. ) 在早实混合样及其个体上扩增出了约900 bp 的特异带OPB208900, 而在晚实混合样及其个体上无此特异带。用OPB208 为引物, 以19 个品种和3 个株系的DNA 为模板进行扩增,特异带OPB208900在17 个早实品种中的12 个个体上出现, 而5 个晚实品种( 系) 上均无此带。 相似文献
5.
晚实核桃优良新品种‘冀丰’ 总被引:2,自引:0,他引:2
‘冀丰’为晚实核桃新品种, 光滑, 仁饱满, 单果质量11. 19 g, 种壳厚1. 14 cm, 出仁率58. 5 %, 粗脂肪含量68. 53%; 丰产优质, 抗逆性强, 适宜华北核桃产区及与之土壤气候条件相近地区栽培。 相似文献
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7.
【目的】探究新疆核桃砧木苗生长特征及生理特性对氮梯度的响应,为核桃砧木苗施氮标准化的探究奠定基础。【方法】以阿克苏厚皮农家种质核桃砧木苗为研究对象,进行不同梯度的氮素诱导,测定核桃砧木苗生长及生理指标。【结果】基于氮梯度加载下的核桃砧木苗株高、地径、叶片数、叶长、光合色素含量、可溶性蛋白质含量、可溶性糖含量、硝酸还原酶活性、谷氨酰胺合成酶活性均呈上升趋势,并在15 g.a-1.m-2处理下达到最高值。【结论】适量地增施氮肥有利于核桃砧木苗的生长、提高砧木苗体内营养物质含量以及促进氮代谢能力。 相似文献
8.
近年来甘肃陇南地区华山松溃疡病发生严重,导致华山松大面积死亡,但有关病害发生的原因等尚不清楚。采用组织分离法从感病华山松的健康部位、病健交界处、子实体及病组织处分离获得97个真菌菌株,结合形态学及系统学对分离到的菌株进行初步分析。结果显示,所得菌株分为9个种,其中Neocosmospora silvicola占14.17%,Fusicolla violacea占1.67%,Microcera sp.占8.33%,Neonectria neomacrospora占11.67%,Dermea pinicola占8.33%,Nematogonum ferrugineum占9.17%,Pezicula sporulosa占4.17%,Phaeoacremonium fraxinopennsylvanicum占14.17%,Sarea resinae占9.17%。以丛赤壳科为优势菌群,占总菌株数量的44.33%;初步推断Neocosmospora silvicola为疑似病原菌。 相似文献
9.
10.
Water stress, with its negative consequences on plant growth and survival, can be mitigated by Azospirillum brasilense inoculation. In tomato, A. brasilense delays wilting caused by a vascular pathogen, Clavibacter michiganensis subsp. michiganensis, by yet unknown mechanisms. We studied morphological, anatomical and physiological changes induced by A. brasilense in tomato that relate to water stress tolerance, which could explain the deferral in symptom expression. For this purpose, tomato seeds were treated or not with A. brasilense BNM65, and 5 weeks later plants were challenged with C. michiganensis subsp. michiganensis or mock inoculated with water. There was a large growth promotion associated to Azospirillum: treated plants had higher total biomass and leaf area. In relation to water stress tolerance, Azospirillum treated plants had larger xylem vessel area, higher stem specific hydraulic conductivity, thicker stems, and lower shoot/root dry matter and specific leaf area. These changes were opposite to those induced by C. michiganensis subsp. michiganensis. We conclude that A. brasilense favoured a better adjustment of plant-water relations by several mechanisms, and thus, transitorily alleviated symptoms expression of a vascular disease. 相似文献