谷子抗除草剂基因的发现及其应用

谷子田间除草一直是阻碍谷子产业化发展的主要因素之一。谷子抗除草剂品种的出现,解决了谷子田间除草难的问题,有利于谷子大面积的栽培,使谷子产业化发展成为可能。本文围绕谷子抗除草剂基因的发现、类型和机理以及抗除草剂品种在谷子生产中的应用情况等方面进行阐述,探讨谷子抗除草剂应用对环境的影响,并对谷子抗除草剂研究中存在的问题及应用前景进行了展望。     

Fluazifop-butyl causes membrane peroxidation in the herbicide-susceptible broad leaf weed bristly starbur (Acanthospermum hispidum)

In order to clarify the action mechanism of fluazifop-butyl, an aryloxyphenoxypropionate (AOPP) herbicide in bristly starbur (Acanthospermum hispidum DC.), a unique fluazifop-butyl-susceptible broad-leaved weed, ethylene evolution and membrane lipid peroxidation in the plant seedlings were investigated. Foliar application of fluazifop-P-butyl induced ethylene evolution only from bristly starbur, but not from oat (Avena sativa L.), another fluazifop-butyl-susceptible species, and two tolerant species, pea (Pisum sativum L.) and hairy beggarticks (Bidens pilosa L.). The other AOPP herbicides, quizalofop-ethyl and fenoxaprop-ethyl, and a cyclohexanedione (CHD) herbicide, sethoxydim, did not enhance ethylene production from bristly starbur. This fluazifop-butyl-induced ethylene production in bristly starbur was completely suppressed by aminoethoxyvinylglycine (AVG), a 1-aminocyclopropane-1-carboxylic acid (ACC) synthase inhibitor, but not by p-chlorophenoxyisobutyric acid (PCIB), an anti-auxin compound, suggesting this evolved ethylene was not auxin-induced. Phytotoxic action by fluazifop-P-butyl (5 μM) in bristly starbur was reduced markedly by two lipid-soluble antioxidants, vitamin E, and ethoxyquin. The ethylene production from the plant was also inhibited by these two antioxidants. Content of malondialdehyde, an indicator of lipid peroxidation, increased only by fluazifop-P-butyl in bristly starbur seedlings but not in oat, and this increase was inhibited by ethoxyquin. These results strongly suggest that the primary site of action for fluazifop-butyl in bristly starbur is on the membranes and active oxygen species and/or free radicals are involved in peroxidation. Ethylene evolution is probably induced by these reactive oxygen species.     

Selective Postemergence Herbicides for Grass Control in Sunflower (Helianthus annuus L.)

Selective postemergence herbicides to control grass weeds in broadleaf crops have become available in recent years. However, the efficacy of these new herbicides in controlling grasses in sunflower has not been extensively evaluated. Field experiments were conducted to evaluate five selective herbicides for their efficacy on crabgrass ( Digitaria sanguinalis L. Scop) and barnyard grass ( Echinochloa crusgalli L. Beav.) control in sunflower ( Helianthus annuus L.) in 1983 and 1984. Commercial sunflower varieties, Interstate 7775 and Stauffer 3101 were planted on Calloway silt loam and Portland clay in 1983 and 1984, respectively. Herbicides were applied when sunflower was 10 cm tall, at 4- to 5-leaf stage, and target grass weeds at the 3- to 4-leaf stage. All herbicides, sethoxydim, xylofop-ethyl, haloxyfop, fluazifop butyl, and CGA 82725, gave good to excellent control of the target weeds. Herbicide injury to sunflower ranged from very minor to none. None of the herbicides greatly affected yield, test weight, seed weight, head diameter or plant height of sunflower. Difference in soil types tended to influence the effectiveness of herbicides.     

Influence of herbicides and felling,fertilization, and prescribed fire on longleaf pine establishment and growth through six growing seasons

Recovery of longleaf pine (Pinus palustris P. Mill.) is necessary to arrest the decline of many associated plants and animals, and the establishment of longleaf pine on much of its original range requires artificial regeneration and diligence. In central Louisiana, USA, two fertilization levels (No [NF] or Yes [F-36 kg/ha N and 40 kg/ha P]) in combination with three vegetation treatments (check, two prescribed fires [PF], or multi-year vegetation control by herbicidal and mechanical means [IVM]) were applied to container-grown longleaf pine plantings in two studies. In Study 1 (grass dominated), 6-year-old longleaf pine survival was 52% on the F–checks, 78% on the F–PF plots, and averaged 93% on the other four treatment combinations. Longleaf pine trees on the IVM plots (3.4 m) were significantly taller than on the other two vegetation treatments, and trees on the PF plots (1.8 m) were taller than trees on the check plots (1.2 m). In Study 2 (brush dominated), survival averaged 65% across the six-treatment combinations after 6 years. The longleaf pine trees were 4.7 m tall on the IVM plots and averaged 3.9 m tall on the check and PF plots. Fertilization increased P concentrations in the soil and longleaf pine foliage, while fertilization did not significantly affect longleaf pine height growth. Native fertility was not apparently limiting longleaf pine development contrary to prior research recommendations for these soils. In both studies, the IVM treatment reduced early herbaceous competition and the number and height of arborescent plants. The PF treatment reduced arborescent plant height on the grassy site where fires were more intense than on the brushy site.     

Optimizing dosage of sethoxydim and fenoxaprop-p-ethyl with adjuvants to control wild oat

Using adjuvants to optimize and increase the efficacy of herbicides is an acceptable manner to reduce herbicides undesirable impact on the environment. Therefore, to detect suitable adjuvants for sethoxydim or fenoxaprop-p-ethyl, two dose-response experiments were conducted separately. The treatments consisted of six doses of sethoxydim or fenoxaprop-p-ethyl with and without adjuvants of Frigate, Citogate and Adigor against wild oat (Avena fatua L.). Moreover, the surface tension of a range of concentrations of adjuvants and adjuvants + sethoxydim or fenoxaprop-p-ethyl aqueous solutions was determined. Lower and higher surface tension values were obtained with aqueous solution of Citogate and Frigate alone and with herbicides. When adjuvants were combined with herbicides, wild oat control was significantly increased and was more than when were used alone. Between evaluated herbicides, adjuvant receptivity for sethoxydim was higher than for fenoxaprop-p-ethyl. Among evaluated adjuvants, the addition of Frigate and Adigor had the lowest and the highest effect, respectively, on the performance of sethoxydim or fenoxaprop-p-ethyl in controlling wild oat, which supports the solubilizing nature of cuticular waxes by Adigor as a theory.