Effects of fire,bruchid beetles and soil type on germination and seedling establishment of Acacia drepanolobium

The effects of fire, bruchid seed beetles and soil type on the germination and seedling establishment of Acacia drepanolobium were experimentally investigated. Seeds subjected to three seed treatments (bruchid damaged, bruchid-free burnt, and bruchid-free unburned) were germinated in three different soil types (black cotton, red sandy, and clay loam soils). Undamaged seeds had significantly higher germination (85.3%) than either beetle damaged (20.6%), or burnt seeds (3.4%). Soil type did not influence germination or seedling survival. Burnt seeds had an average germination of 2% in clay loams and 5.4% in red sandy soils, while beetle damaged seeds had an average germination of 15.6% in clay loams and 28.7% in black cotton soils. Treatment effects on seedling mortality or survival were not significant but seedlings from undamaged seeds had higher survival rates than those damaged by bruchid beetles.     

人工合成甘蓝型油菜游离小孢子培养及其植株再生研究初报

利用游离小孢子培养技术对5个人工合成甘蓝型油菜品系进行了离体小孢子培养。从大田非控温控光条件下生长的供体植株第一朵花开后3～12d,取长度2．5～3．5mm的花蕾分离小鬼子,在NLN13液体培养基中进行薄层培养诱导成胚状体,然后用无激素的MS培养基诱导植株再生。对不同的供试材料、取蕾时期和培养基蔗糖浓度对小孢子胚胎发生能力的影响作了初步研究。供试的5个材料中有4个对培养有反应,但不同材料的小孢子胚胎发生能力有较大的差异。小孢子取样的时期以主花序第一朵花开后6d左右为宜,培养基蔗糖浓度以13％的效果最好。     

巨桉下胚轴诱导不定芽与植株再生的研究

研究了以巨桉 (Eucalyptusgrandis)的子叶、下胚轴作外植体诱导愈伤组织产生不定芽及植株再生的过程 ,结果表明 :下胚轴在改良H培养基 +2 .4 -D 1 0mg/L +BA 1 0mg/L +NAA 0 1mg/L的诱导下容易获得有诱导价值的愈伤组织 ,并在改良H培养基 +BA 1 0mg/L +NAA 0 1mg/L的诱导下发生月增殖率 8～ 10倍的丛生苗     

马尾松低产林采伐和改造试验技术研究

通过１１．６公顷马尾松低产林采伐改造营林试验，初步筛选出马尾松低产林采伐 方式和树种配置最优组合类型；针对农区人口稠密、交通方便这一特点，在造 林三年内实行矮秆作物间作措施，既解决幼林抚育和保护需要经费，又给农民 增加一定的经济收益。该项技术适合目前四川丘陵马尾松林区生产现状．     

棉花原生质体培养研究进展（综述）

本文在介绍棉花原生质体培养研究历史的基础上，综述了棉花原生质体分离、培养及植株再生中的一系列问题，并介绍了棉花原生质体培养在遗传改良中的应用。