OVER- EXPRESSION OF AN AUX1 HOMOLOG TRIGGERS OVARY INITIATION IN VITRO OF POPULUS TOMENTOSA CARR.

INTRODUCTIONPolar transport is one key feature of auxin action was believed as a positional signal plays a wide arrange ofroles in plantgrowth and development,such as the gravity response[1],the initiation of lateral roots[2],positiondetermination of leaf primordial[3,4],differentiation of flower primordial[5],the axis formation in developingembryos[6~8],the patterned differentiation of vascular tissues[9~11].Polar auxin transport(PAT)system contributes indole-3-acetic acid(IAA)thorough …     

一种特殊情况的焦点量计算问题

为了讨论微分方程系统是否存在极限环，常常归结为讨论系统的细焦点等问题。用极坐标方法，讨论一类特殊系统的焦点量。化简了二阶及三阶焦点量公式：ｒ５（２π）＝∫０＾２π Ｒ５ｄθ；ｒ７（２π）＝∫０＾２πＲ７ｄθ＋２∫０＾２πＲ５ｒ３ｄθ；并且具体计算出这类系统的二阶焦点量。此类系统的二次项中有两个可变参数ｆｙ＾２（０，０），ｇｘ＾２（０，０）可供调解。     

高效液相色谱法分析黄瓜子叶极性脂

通过HPLC的二次操作,可将黄瓜子叶极性脂快速、经济地分离成各脂成分,并进一步分离成各分子种。通过第1次操作,黄瓜子叶极性脂被分离成各脂组分:单半乳糖二甘油脂(MGDG),双半乳糖二甘油酯(DGDG),磷脂酰乙醇胺(PE),磷脂酰胆碱(PC),磷脂酰甘油(PG),磷脂酰肌醇(PI)和磷脂酸(PA)等;第2次经HPLC系统后,各脂组分被分离成C18∶3/C18∶3、C18∶2/C18∶3、C18∶2/C18∶2、C16∶0/C18∶3、C16∶0/C18∶2等分子种。     

Vigilance behaviour of polar bears (Ursus maritimus) in the context of wildlife-viewing activities at Churchill, Manitoba, Canada

Viewing of polar bears (Ursus maritimus) from tundra vehicles has been offered at Churchill, Manitoba since the early 1980s. This form of wildlife viewing has provided a unique and safe way for tourists to learn about polar bears. However, these activities have largely been carried out without examining possible effects on polar bear behaviour. We studied vigilance behaviour (a scanning of the immediate vicinity and beyond) of resting polar bears to evaluate impacts from tundra vehicle activity. Focal animal sampling was used to examine whether a difference in vigilance behaviour existed when vehicles were present. We recorded the numbers of head-ups, vigilance bout length, and between-bout intervals for polar bears. In general, the frequency of head-ups increased, and the between-bout intervals decreased for male bears, when vehicles were present. Female bears behaved opposite to males. The vigilance bout lengths did not differ significantly between vehicle presence and absence. Vigilance behaviour of male bears was not magnified with increasing numbers of vehicles; therefore the threshold is one vehicle. We suggest that manipulative studies be conducted to examine how distances between vehicles and bears, tundra vehicle activity in the immediate vicinity of a bear during viewing, and noise of tourists affect increased vigilance.     

毛白杨PtAUX1超表达诱导体状胚发生

体状胚的发生由一个生长素信号级联系统控制,其发生过程需要大量的生长素,随后在去除生长素或降低生长素浓度的条件下,体状胚趋于成熟。除植物激素外,环境胁迫也是体状胚发生所必须的,激素和环境胁迫协同诱导细胞的脱分化、再分化和体状胚的发生。目前发现的参与体状胚发生的主要基因有SERK、PKL和BBM等,它们作用于体状胚的不同发育阶段。本研究中,在毛白杨35S::PtAUX1超表达植株中发现叶片表面产生大量体状胚,体状胚分散在叶片主脉及侧脉两侧或聚集在叶片边缘,其中部分体状胚可发育成成熟体状胚。将成熟体状胚取下,置于MS0培养基上进行培养,体状胚发芽并缓慢产生根。研究结果表明,PtAUX1通过建立新的生长素浓度梯度,诱导体细胞转化成胚性细胞,从而使叶片产生出体状胚。除上述基因外,PtAUX1也参与体状胚的发生,这一研究发现现尚未见报道。     

6_二甲基氨基嘌呤诱导太平洋牡蛎三倍体_抑制受精卵第一极体释放

于 1996～ 1997年 ,用 6-二甲基氨基嘌呤 ( 6-DMAP)抑制受精卵第一极体的释放 ,诱导太平洋牡蛎产生三倍体。选用L16( 45)设计 ,进行三因素四水平的正交试验 :6-DMAP浓度 ,设15 0、30 0、45 0和 60 0μmol/L ;诱导时机 (即精卵混合后的时间 ) ,设 10、15、2 0和 2 5min ;诱导持续时间 ,设 10、15、2 0和 2 5min。试验平行重复二次。结果为最高三倍体诱导率为 ( 71.3± 1.2 ) % ,该实验组胚胎孵化率为 ( 5 5 .5± 3.1) % ,D形幼虫畸形率为 ( 10 .7± 1.6) %。根据直观分析结果 ,得出诱导太平洋牡蛎三倍体各因素的最优水平组合 :水温 2 5～ 2 5 .5℃ ,精卵混合 10min时 ,将受精卵浸泡在含 6-DMAP 60 0μmol/L的海水中 15min ;决定三倍体产生的三因素的主次顺序 :6-DMAP浓度→诱导时机→诱导持续时间。 6-DMAP浓度对三倍体诱导率影响显著 ,但诱导时机和诱导持续时间不显著。     

Automatic counting of annual rings on Pinus sylvestris end faces in sawmill industry

One of the essential requirements of current agricultural practice is the need to evolve towards crop techniques that make better use of productive factors behaving in sustainable and environmentally respectful ways. Pest control is one of the most important problems to take into account, due to the significant production losses that pests may cause. The Integrated Production for olive crop defines a set of rules that has to be followed in order to ensure a production of higher quality, and promotes an environmentally respectful model of Olive cultivation. This work presents SAIFA (spanish acronym for Sistema de Alerta e Información Fitosanitaria Andaluz - Andalusian Phitosanitary Information and Alert System) a web-based information system which allows monitoring the Integrated Production for the olive crop in Andalusia, Spain. SAIFA has been built as a tool to assist agricultural technicians to comply with the quality standards of Integrated Production, and to help decision makers to choose the actions to be performed in the crop. Also, it assists coordinators to choose an Integrated Production strategy applicable for the whole region with the main objective of ensuring the safety of crops and reporting the current phitosanitary state to the authorities.     

2009年6月黑龙江省低温阴雨天气成因初步分析

利用美国国家环境预报中心（NCEP）再分析资料以及74项环流指数等资料,对2009年6月黑龙江省连阴雨天其成因进行初步分析。结果表明,此次连阴雨天气过程与冷空气活动密切相关,是中高纬系统相互作用的结果。鄂霍次克海和乌拉尔山附近地区存在明显的正高度异常,贝加尔湖附近为负高度异常,500hPa位势高度场上欧亚中高纬度地区纬向呈＂＋-＋＂型,这种高度异常形势为鄂霍次克海阻塞形势的形成和维持提供了大尺度环流背景,使东北冷涡长时间影响黑龙江省。在欧亚大陆中高纬度长期出现阻塞形势的背景下,加上低空充足的水汽供应,黑龙江省连续经历了4次冷涡天气造成了此次低温阴雨天气过程。     

圈养条件下北极熊的饲养繁殖

对大连森林动物园圈养条件下北极熊的饲养管理、发情交配、繁殖及幼仔人工育幼等方面进行了研究与分析,为北极熊在动物园的饲养与繁殖,尤其在人工育幼方面取得了大量的基础数据,希望对北极熊的圈养繁殖起到一定的推动作用。     

Quercetin Promotes Auxin Transport in Arabidopsis thaliana

Study on the role of quercentin in polar auxin transportation. Arabidopsis was cultured on medium supplemented with quercetin to observe the growth of hypocotyls, 14C-IAA transport assays were conducted to measure the auxin transport activity. The results showed that Arabidopsis mutant auxl which had been deficient in auxin influx transportion obviously recovered the ability after cultured on the medium with quercetin. The polar auxin transport was promoted by the addition of quercetin. These results indicated that quereetin could promote polar auxin transport in vivo.