THE INSENSITIVITY OF 99mTc PERTECHNETATE FOR DETECTING METASTASES OF A FUNCTIONAL THYROID CARCINOMA IN A DOG

This report describes the use of ^99mtechnetium pertechnetate (^99mTcO₄⁻) and ¹³¹[for imaging of a metastatic thyroid carcinoma in a dog. The ¹³¹] imaging showed metastatic lesions undetected by the ^99mTcO₄⁻ imaging on 2 separate occasions. The possible mechanisms for the discrepancies between ¹³¹I and ^99mTcO₄⁻ imaging of thyroid carcinomas are discussed. The use of ¹³¹I for the imaging of functional thyroid carcinomas in the dog is recommended.     

Different biological behaviour of Waldenström macroglobulinemia in two dogs

Waldenström Macroglobulinemia is a low‐grade immunosecretory disorder associated with lymphoid tumours, which is rarely reported in veterinary medicine. In this study, we describe two clinical cases of this rare syndrome in dogs, each characterized by a different onset and clinical course. In one case, a hyperacute onset and aggressive behaviour of the neoplasm was observed. Absolute serum viscosity (SV) was retrospectively evaluated in order to explain clinical findings. Rotational viscosimetry showed good precision in measuring SV. Both dogs had SV values higher than a control groups of healthy dogs although only one subject developed hyperviscosity symptoms and complications. At high paraprotein concentrations, a slight reduction of the M‐component was associated with a marked decrease in SV. Thus, this work suggests that SV assessment is a relevant tool for managing monoclonal gammopathies, whose usefulness should be further confirmed in larger cohorts of dogs.     

滇油杉林下死可燃物含水率与温湿度关系模型研究

[目的]研究滇油杉林下死可燃物含水率与温湿度关系模型.[方法]以滇油杉林下三类死可燃物为研究对象,借助于恒温恒湿箱对不同温湿度组合下的含水率进行测定,并研究三类死可燃物含水率与温度、湿度、温温度的关系.[结果]在相同湿度条件下,死可燃物含水率随温度的升高而降低,但在不同的湿度下具有不同的变化规律;在相同温度条件下,死可燃物含水率随相对湿度的增大而增大,但在不同的温度下具有不同的变化规律;温湿度共同作用影响死可燃物的含水率,且不同的死可燃物有不同变化规律.[结论]可燃物含水率是受多种因素影响.滇油杉林下三类死可燃物含水率从小到大依次为粗大死可燃物、腐殖质、细小死可燃物.     

m6A甲基化在鸡肌肉生长发育中的表达研究

试验旨在研究RNA m6A修饰相关基因去甲基化酶Alk B同源蛋白5（Alk B homologue 5，ALKBH5）、去甲基化酶肥胖相关蛋白（fat mass and obesity-associated protein，FTO）、甲基转移酶样蛋白3（methyltransferase like 3，METTL3）、甲基转移酶样蛋白14（methyltransferase like 14，METTL14）和成肾细胞瘤1-结合蛋白（Wilms’tumor 1-associating protein，WTAP）在鸡骨骼肌发育过程中的表达，分析其与骨骼肌m6A甲基化水平的相关性。首先，利用实时荧光定量PCR技术检测m6A甲基化相关基因在金茅花鸡12（E12）、14（E14）、16（E16）、18（E18）胚龄和1日龄腿肌和胸肌组织中mRNA表达水平，以及其在鸡成肌细胞50%、100%增殖期和1、2、3、4、5 d分化期的mRNA表达水平；随后，利用m6A甲基化试剂盒检测金茅花鸡E12和1日龄腿肌和胸肌组织中m6A甲基化修饰水平，与m6A甲基化相关基因表达水平进行相关性分析。结果显示，m6A去甲基化基因ALKBH5和FTO mRNA表达水平在骨骼肌发育过程中显著上调（P<0.05），即在E12、E14低表达，E16、E18逐渐上调，1日龄达到最高。m6A甲基化写入基因METTL14、METTL3和WTAP mRNA表达水平在E12、E14、E16逐渐上升，E18下降，随后至1日龄表达量回升。在细胞增殖过程中，ALKBH5、FTO、METTL14、METTL3和WTAP基因表达均上调；在细胞分化过程中ALKBH5和FTO基因表达水平显著上调（P<0.05），在分化第5天达到最高。METTL14、METTL3和WTAP基因mRNA表达水平在细胞诱导分化的1、2、3、4 d表达量呈下降趋势，而在诱导分化的第5天有所回升。甲基化水平检测结果显示，腿肌和胸肌m6A甲基化水平变化趋势一致，均在胚胎发育过程中显著下降（P<0.05），至1日龄达到最低。相关性分析结果显示，鸡骨骼肌RNA m6A甲基化水平与m6A去甲基化修饰基因ALKBH5、FTO mRNA表达水平呈显著负相关（P<0.05）。综合以上试验结果，推测m6A甲基化修饰与鸡骨骼肌发育相关，而去甲基化基因ALKBH5、FTO可能通过调控RNA m6A甲基化水平，影响鸡骨骼肌发育。本研究结果为进一步研究m6A甲基化修饰调控鸡骨骼肌生长发育的功能和分子机制提供理论依据。     

新型吸水剂在砂姜黑土甘蓝包菜上应用效果

研究了一种新型吸水剂在砂姜黑土应用效果。结果表明：新型吸水剂对生长初期甘蓝包菜生长有一定的抑制作用,但随着水分胁迫增加,吸水剂保水效果的发挥,包菜的生长很快恢复,到收获期,包菜产量,水分利用效率,土壤保水能力都显著高于对照处理。吸水剂以深施效果最好,增产率达15.5%,水分利用效率提高48.3%,供试的吸水剂在抗旱节水上有很好的应用前景。     

种子含水率在线测量系统

根据种子含水率的测量要求，研制了一种可同时测量电容、质量和温度3个参量的装置和测量电路。该测量系统的机械装置由2个同心圆筒组成，内筒的旋转运动实现被测种子的动态喂料。采用抗寄生电容效应的开关电容电路测量上、下极板之间的电容；下极板同时也是称量平台；温度传感器测量种子的温度。3个测量值经A／D转换电路以数字量输出。分别用3个电路单独测量已知的电容、质量和温度，以及不同温度和相对湿度条件下对电容测量值的影响，并据此分析了各测量单元的精度、可靠性和可行性。     

秸秆覆盖对季节性冻融期土壤水分特征的影响

以北方高寒区典型城市哈尔滨市为研究区域,通过冬季大田试验(试验周期2013-11-01—2014-04-30),设置自然无覆盖、覆盖秸秆厚度5、10、15 cm 4个处理,分别测定不同处理下20、40、60、100、140、180 cm深度土壤液态含水率以及气象数据,分析季节性冻融期秸秆覆盖对土壤水分变化特征的影响。研究结果表明:秸秆覆盖使0~60cm土层内液态含水率增加或减小的时间拐点发生延迟,随着秸秆覆盖厚度的增加其延迟效果越明显,但土壤冻结期的延迟效果比冻土融化期明显;秸秆覆盖阻碍了冻土融化初期融雪水入渗,使自然无覆盖处理液态含水率在20、40、60 cm土壤深度出现短暂的峰值,而在冻土融化末期秸秆覆盖抑制了土壤水蒸发,使各秸秆覆盖处理液态含水率在20、40、60 cm土壤深度又高于自然无覆盖。秸秆覆盖可有效平抑冻融期0~60 cm土层土壤液态含水率的变化幅度,且随着土壤深度的增加其平抑效果具有减弱趋势;积雪融水和秸秆覆盖的双重作用能够有效增加土壤墒情,但其增墒能力随着土壤深度的增加而降低,不同秸秆覆盖处理对0~60 cm土层的平均增墒能力由大到小排序依次为:15、10、5 cm。     

丽江牡丹不同品种的生物学特性及耐水淹胁迫能力

对丽江紫、丽江粉和香玉板3个丽江牡丹品种的物候、枝条生长量及成花率进行了观测分析,结果表明:3个品种在物候特征方面差异较大,香玉板的生长发育节律和中原牡丹相似性较高,丽江紫的物候晚于中原牡丹品种,丽江粉则早于中原牡丹品种;香玉板枝条生长量达42.9cm,丽江紫和丽江粉分别达32.3、30.1cm,在丽江地区,丽江紫、丽江粉和香玉板的枝条实存率约为42%~53%,33%~46%和38%~52%。从生殖发育的特点看,香玉板与中原牡丹同属枝叶同放型,成花率和开花率高,而丽江紫和丽江粉花蕾有败育现象,花蕾发育晚于叶。在品种抗水淹能力评价方面,运用NR活性、RPP的变化和叶绿素总量下降率指标进行分析,结果表明:丽江紫抗水淹力最强,丽江粉次之,香玉板抗水淹能力较差。     

Determination of primary structure of amberjack myosin heavy chain and its relationship with structural stability of various fish myosin rods

The structural stability of fish myosin depends upon species and temperatures of water in which fish live. Primary, secondary, and quaternary structures of myosin heavy chain (MyHC) from three species of fish living at different temperature ranges have been compared with those of rabbit MyHC in order to investigate the differences in stability. Primary structure of MyHC, although being accessible for warm-water and cold-water fish (carp and walleye pollack), was not available in previous for tropical-water fish literature; so in this study primary structure of MyHC of the tropical-water fish amberjack has been determined by cloning and sequencing its cDNA. The MyHC has 1938 amino acid residues (AA), which are almost as much as as those of carp and walleye pollack. The amberjack MyHC is 91–95% homologous with other fish and rabbit MyHCs. There is a discernible difference between animal species with stable myosin rod (amberjack, carp, and rabbit) and walleye pollack with unstable rod. Stable rod species have a high probability of forming coiled-coil around the COOH-terminal end of the rod, while the pollack has a low coiled-coil formation probability. In addition, the average scores of the coiled-coil for myosin rod were rabbit (1.738) > amberjack (1.691) > carp (1.680) > walleye pollack (1.674) which correlated exactly with the observed stability. The results suggest that coiled-coil forming ability, particularly around the COOH-terminal end, directs structural stability of fish myosin rod.     

牧场土壤含水率与坚实度空间变异与相关性分

利用土壤水分/圆锥指数复合测量装置,应用精细农作技术体系网格定点测量与GPS定位,在一块面积约1.27 hm2的草地上获取了土壤含水率与坚实度空间分布基础数据,并针对采样过程中出现的数据缺失,用偏最小二乘法对数据进行修补.然后运用克里格插值法进行数字化成图,并在此基础上分别对含水率与坚实度的空间变异性及两者的相关性做了分析.