东方百合L_sCCR1基因的克隆及表达分析

百合茎秆的挺度直接关系到百合花采收后的货架寿命等重要商品性状,而这些商品性状与植物茎秆中的木质素的含量高低有关.本文应用RLM-RACE技术,从东方百合索蚌植株中克隆了木质素合成相关基因一肉桂酰辅酶A还原酶,定名为LsCCR1.该基因cDNA全长为1 499 bp,包括完整的阅读框,编码388个氨基酸.多重比对分析发现百合LsCCR1基因与其他植物上已经发现的CCR基因同源性多介于55%～66%之间.利用半定量PCR检测LsCCR1基因在百合不同组织中的表达差异,结果显示LsCCR1基因主要在百合的茎秆中表达,根部次之,叶片、鳞茎及花蕾中表达量较少,其表达模式与拟南芥、桉树及大麦中CCR基因的表达模式类似.     

Infrared spectroscopic study of alkaline oxygen treatment of lignin with ATR technique in aqueous state 1: method for determining quantitative spectra of oxygen-degraded lignin

For the fundamental study of oxygen delignification of kraft pulp, structural changes of kraft lignin during alkaline oxygen treatment were investigated with the use of infrared measurement with attenuated total reflectance (ATR) technique. In the neutralized reaction mixture of alkaline oxygen-treated kraft lignin, there is a significant amount of NaCl, so that the spectral changes of water due to the coexistence of NaCl was investigated, and how to remove the huge absorption of NaCl solution is discussed. Sodium vanillate–NaCl solutions were employed as model solutions for the reaction mixture. Partial least square (PLS) regression was applied for the prediction of NaCl concentration, and the spectrum of NaCl solution was subtracted from the spectrum of sodium vanillate–NaCl solution as background measurement. This allowed us to obtain the vanillate spectra free from the absorption of NaCl solution. In addition, the mathematical method for reconstructing the spectrum of NaCl solution is discussed. The spectrum of NaCl solution is reconstructed as the linear combination of basic spectra calculated by singular value decomposition (SVD), and it was subtracted from that of the sodium vanillate–NaCl solution. By this procedure, the vanillate spectra were also obtained quantitatively, as has been demonstrated in PLS regression study. It was also confirmed that the quantitative spectra of high molecular weight fraction of alkaline oxygen-treated kraft lignin were obtained by the use of this reconstruction technique.Parts of this report were presented at the 52nd Annual Meeting of the Japan Wood Research Society, Gifu, Japan, April 2002 and the 12th International Symposium on Wood and Pulping Chemistry, Madison, USA, June 2003     

脲醛树脂的纳米级改性研究

介绍了蒙脱土、有机蒙脱土、木质素三种物质对脲醛树脂的改性过程并对改性后脲醛树脂的各项性能进行了测试。结果表明:蒙脱土通过插层处理变为有机蒙脱土后,能显著改善脲醛树脂的性能指标。木质素和未改性的蒙脱土对改善脲醛树脂胶黏剂游离甲醛释放量几乎无影响。     

Characteristics and decomposition rates of pruning residues from a shaded coffee system in Southeastern Brazil

In the Zona da Mata Mineira of Southeastern Brazil the development of sustainable land requires the integration of crops with trees. The objectives of this study then were to (i) characterize prunings from the main tree species in an agroforestry system; (ii) determine the effects of the physical and chemical characteristics of the prunings on their decomposition patterns in the laboratory; (iii) assess the effect of mixing leaves of different species on decomposition rates; and (iv) propose a decomposition index for the residues studied. The study was carried out with pruning residues from Cajanus cajan, Solanum variable, Cassia ferruginea, Piptadenia gonoacantha, Croton urucurana, and Melinis multiflora. The materials were characterized for total C, N, P, Ca, Mg and K contents; lignin, cellulose, hemicellulose and soluble polyphenols contents. The pruning residues had high polyphenols and lignin contents, high C:N and C:P ratios, and low contents of Ca, Mg, and K. The low decomposition rates of the prunings were related to the P, K, hemicellulose and polyphenol contents. The rates of N mineralization from most of the residues indicate that there is a potential to supply the needs of a crop of maize. The residues of some species, if decomposed alone, would not supply sufficient nutrients, and need to be mixed with leaves of other species.This revised version was published online in November 2005 with corrections to the Cover Date.     

Reaction behavior of lignin in supercritical methanol as studied with lignin model compounds

 The reaction behavior and kinetics of lignin model compounds were studied in supercritical methanol with a batch-type supercritical biomass conversion system. Guaiacol, veratrole, 2,6-dimethoxyphenol, and 1,2,3-trimethoxybenzene were used as model compounds for aromatic rings in lignin. In addition, 5-5, β-1, β-O-4, and α-O-4 types of dimeric lignin model compounds were used as representatives of linkages in lignin. As a result, aromatic rings and 5-5 (biphenyl)-type structures were stable in supercritical methanol, and the β-1 linkage was not cleaved in the β-1-type structure but converted rapidly to stilbene. On the other hand, β-ether and α-ether linkages of β-O-4 and α-O-4 lignin model compounds were cleaved rapidly, and these compounds decomposed to some monomeric compounds. Phenolic compounds were found to be more reactive than nonphenolic compounds. These results indicate that cleavages of ether linkages mainly contribute to the depolymerization of lignin, whereas condensed linkages such as the 5-5 and β-1 types are not cleaved in supercritical methanol. Therefore, it is suggested that the supercritical methanol treatment effectively depolymerizes lignin into the lower-molecular-weight products as a methanol-soluble portion mainly by cleavage of the β-ether structure, which is the dominant linkage in lignin. Received: December 19, 2001 / Accepted: April 30, 2002 Acknowledgments This research has been done under the research program for the development of technologies for establishing an ecosystem based on recycling in rural villages for the twenty-first century from the Ministry of Agriculture, Forestry and Fisheries, Japan; by a Grant-in-Aid for Scientific Research (B)(2) (no.12460144, 2001.4–2003.3) from the Ministry of Education, Culture, Sports, Science and Technology, Japan; and under the research program from Kansai Research Foundation for Technology Promotion, Japan. The authors thank them for their financial support. This study was presented in part at the 45th Lignin Symposium, Ehime, Japan, October 2000 and the 52nd Annual Meeting of the Japan Wood Research Society, Gifu, Japan, April 2002 Correspondence to:S. Saka     

酶活化处理条件及其对松木纤维胶合性能的影响初探

通过测定漆酶处理纤维压制纤维板的内结合强度 ,观察到漆酶对云南思茅松木纤维的活化作用及漆酶处理对木纤维自身胶合的贡献。不同pH值条件下漆酶处理木纤维的内结合强度显著高于水处理纤维压制的板材强度。失活后的酶液与水等效 ,说明对实现木材自身胶合起作用的主要是漆酶本身。初步探索了pH值、酶剂量、底物浓度、活化处理时间等因素对胶合效果的影响。采用试验得到的较好活化条件压制的板材内结合强度可达1 0MPa以上     

Structural characteristics of cell walls of kenaf (<Emphasis Type="Italic">Hibiscus cannabinus </Emphasis>L.) and fixation of carbon dioxide

 Kenaf (Hibiscus cannabinus) plants are widely known for their contribution to the global and regional environment because of their ability to fix CO₂. On the other hand, some scientists have doubts about CO₂ fixation by kenaf and have misgivings about the effect of kenaf on the ecosystem. We have characterized the structural characteristics of cell walls of bast fibers, cores, roots, and leaves of kenaf during the maturation of plants and investigated the rate of photosynthesis. During maturation of the kenaf plant the cellulose (bast fiber 52–59%, core 44–46%) and lignin (bast fiber 9.3–13.2%, core 18.3–23.2%) contents increased significantly. The aromatic composition of the lignin of bast fiber was significantly different from that of the core lignin and of other plants. The lignin of bast fiber appears similar to pure syringyl lignin. Fixation of CO₂ by kenaf plants and their contribution to the global environment are discussed. A significatly high rate of photosynthesis of kenaf plants was observed compared to that of woody plants in Japan, but the amount of CO₂ fixation depends on the characteristics of the plantation. If the kenaf was planted in high density, about twice as much CO₂ was fixed as was fixed by trees in a tropical rain forest. Received: April 22, 2002 / Accepted: July 24, 2002 Acknowledgments This project was supported by the Science and Technology Agency (STA) fellowship of the Japan International Science and Technology Exchange Center (JISTEC), which has been successfully applied by Dr. Shuji Hosoya, Forestry and Forest Products Research Institute. We thank Dr. Toshio Sumizono and Mr. Masao Sakurai, Forestry and Forest Products Research Institute, for their kind help in determining the rate of photosynthesis and cultivating the kenaf plants, respectively. We also express our appreciation to Dr. Quang Hung Le, College of Agriculture and Forestry, Ho Chi Minh City, Vietnam for offering information about the cultivation of kenaf at Thu Duc District, Ho Chi Minh City.     

Effects of <Emphasis Type="Italic">Ips typographus</Emphasis> (L.) damage on litter quality and decomposition rates of Oriental Spruce [<Emphasis Type="Italic">Picea Orientalis</Emphasis> (L.) Link.] in Hatila Valley National Park,Turkey

This study investigated the effects of Ips typographus (L.) damage on initial litter quality parameters and subsequent decomposition rates of oriental spruce tree species [Picea orientalis (L.) Link]. The needle litter was collected from highly damaged, moderately damaged and control stands on two aspects (north and south) and two slope position (top and bottom) on each aspect. The litter was analyzed for initial total carbon, lignin and nutrient (nitrogen, phosphorus, potassium, calcium, magnesium and manganese) concentrations. The variability in nitrogen and calcium concentrations and ratios of C:N, lignin:N and lignin:Ca was significantly affected by the insect damaged levels. While nitrogen concentrations in needle litter increased with increasing insect damage (and consequently the ratios of C:N and lignin:N decreased), calcium concentrations decreased (and consequently the ratio of lignin:Ca increased). Aspect and slope positions explained most of the variability in carbon, lignin, phosphorus, potassium, magnesium and manganese concentrations and lignin:P ratio between all studied stands. Litter decomposition was studied in the field using the litterbag technique. The litter from highly damaged stands showed highest decomposition rates followed by moderately damaged and control stands. The mass loss rates were significantly positively correlated with initial nitrogen concentration and negatively with C:N and lignin:N ratios. The effects of microclimate resulting from canopy damage on litter decomposition was also examined at the same time using standard litter with the same litter quality parameters, but they showed no significant differences among the insect damage levels indicating that alteration of the litter quality parameters produced by I. typographus damage played a more important role than altered microclimate in controlling needle litter decomposition rates. However, changes in microclimate factors due to topography influenced decomposition rates.     

At5g54160 gene encodes <Emphasis Type="Italic">Arabidopsis thaliana</Emphasis> 5-hydroxyconiferaldehyde <Emphasis Type="Italic">O</Emphasis>-methyltransferase

The function of an Arabidopsis thaliana gene, At5g54160 annotated as a caffeic acid O-methyltransferase CAOMT gene was characterized. The recombinant enzyme of this gene (AtOMT1) catalyzed the O-methylation of phenylpropanoid and flavonoid substrates. The specificity constants (k _cat/K _m) for 5-hydroxyconiferaldehyde (5-HCAld) and quercetin were both 0.11 μM⁻¹·min⁻¹. On the other hand, lignins of At5g54160-knockout Arabidopsis mutants lacked syringyl units. In addition, we showed that the gene silencing also resulted in significant accumulation of caffeyl alcohol (CaAlc). These results strongly suggested that At5g54160 gene is involved in syringyl lignin synthesis for the methylation of both 5-hydroxyconiferaldehyde and 3,4-dihydroxyphenyl compound(s). Part of this work was presented at the Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry, March 24–27, 2007     

Application of cationic polymer prepared from sulfuric acid lignin as a retention aid for usual rosin sizes to neutral papermaking

Cationic polymers that acted as the retention aids for usual rosin sizes in neutral papermaking were prepared from sulfuric acid lignin (SAL), one type of acid lignin. To convert SAL to the cationic polymer (MP-SAL), SAL was phenolated and then treated by the Mannich reaction to introduce the amino groups. In the MP-SAL single system, MP-SAL exhibited high sizing effectiveness in neutral papermaking with the rosin emulsion size. However, MP-SAL showed no sizing effectiveness when soap rosin size was used. MP-SAL showed increased sizing effectiveness as the pK _a of the introduced amino group increased. From this and comparison of the sizing degrees of MP-SAL and polyethylenimine, which possesses a linear structure, it was suggested that the sizing effectiveness was not only affected by the charge density and molecular weight, but also by the basicity of the introduced amino groups and the molecular structure of the retention aid. In the alum–MP-SAL dual system, alum and MP-SAL synergistically enhanced the sizing effectiveness in the rosin emulsion sizing at neutral pH. In turn, this allowed a decrease in the MP-SAL dosage and resulted in a small decrease in brightness.Part of this report was presented at the 48th Lignin Symposium, October 2003, Fukui