基于SCS-CN和GIS的潜在集雨工程位置确定

合理开发利用雨水资源,是缓解水资源紧张状况的有效途径之一。该文采用SCS-CN水文模型计算流域地表径流量;应用RS技术获取土地利用现状数据,在GIS软件平台下对研究区土地利用现状、土壤类型、地表径流量及数字高程等空间信息数据进行处理分析;根据雨水集蓄利用工程技术规范和坡面集水蓄水工程建设一般的选址要求,构建集雨工程位置选择的约束集;结合空间信息和属性数据及约束集,在GIS软件平台下确定潜在的集雨工程的位置。得出结论:适宜修建蓄水池的位置128处、适宜修建拦水坝的位置47处、适宜修建大口井的位置65处。结果可用于指导当地地表径流资源的科学利用,为山区流域综合治理和集雨工程的规划与设计提供科学依据。     

沼气工程地理信息系统的设计与实现

[目的]设计并实现一种沼气工程地理信息系统。[方法]在对北京市大中型沼气工程充分调查后获得数据的基础上,分析系统用户需求和功能需求,划分子系统功能,使用SuperMap IS.NET和C#语言编程技术,采用WebGIS架构,设计了系统空间和属性数据库,实现了以北京市为例的沼气工程WebGIS系统。[结果]该系统为沼气工程信息的网络发布提供了应用平台。用户可根据系统使用权限实现沼气工程信息的反馈提交和查询等,能更直观、全面、准确地查询工程数据,对数据进行综合分析,从而对沼气工程的现状和变化趋势进行研究和决策。[结论]该系统为相关管理部门对沼气工程的规划管理工作提供了便利服务。     

基于无线传感器网络和WebGIS的墒情监测系统

为了解决墒情监测中便携式监测仪数据存储、发送困难及墒情实时监测和分析等问题,建立了基于无线传感器网络和WebGIS的墒情监测系统。系统以STC单片机为核心芯片,结合GPRS模块、GPS模块,通过无线传感器网络技术,实现土壤水分数据的实时监测及无线传送。建立了数据库,接收监测点数据。并以SuperMap IS.NET和SQL Server 2005为开发平台进行二次开发,将GIS技术与墒情监测结合起来,根据作物耕作深度、作物生长期对土壤含水量进行判断,得出墒情结果。实现墒情监测点信息、土壤含水量曲线图、等值线分布图在Web服务器上的显示。墒情监测系统的建立和使用,对农业生产有深远的意义。     

基于SuperMap IS的棉田土壤肥力信息管理及施肥决策系统

以新疆兵团农七师125团为例,探讨了如何利用SuperMap IS来设计农业网络地理信息系统.利用SuperMap IS实现数据库的连接、数据存储以及空间数据、属性数据的查询和编辑,开发出集棉田土壤养分管理、专家施肥推荐、SQL信息查询以及远程土壤养分数据管理等功能于一体的棉田土壤养分信息管理与作物推荐施肥系统.     

临时收储政策退出对玉米期货和现货价格发现功能的影响

借助改进的蛛网模型,分析了临时收储政策退出对我国玉米期货和现货市场价格发现功能的影响机理;并综合运用滚动协整、VECM、BEKK-GARCH与IS模型,系统探究了2009-2019年我国临储政策及其改革对玉米期货和现货市场价格发现功能的影响.研究发现:1)我国玉米期货和现货价格存在相互引导关系.2)BEKK-GARCH...     

Mononuclear phagocyte receptors in rhesus monkeys (Macaca mulatta) and their role in hemolytic disease of the newborn

Hemolytic disease of the newborn does not develop in rhesus monkeys because placentally-transferred maternal antibodies do not induce immune clearance of the newborn's erythrocytes. In an in vitro RBC adherence assay, rhesus peripheral blood monocytes did not bind newborn's RBC which had been coated in utero or in vitro with maternal antibodies. Nevertheless, rhesus phagocytes possess receptors that are specific for the Fc portion of IgC and for the C3b. Using purified human IgG subclasses as inhibitors of RBC adherence, rhesus Fc receptors preferentially bind IgG1 and IgG3. Thus, it may be that maternal antibodies are non-opsonic because they belong to IgG subclasses that do not bind effectively to rhesus Fc receptors. Also, RBC adherence appears to be controlled by the level of antibody coating which in turn is determined by avidity of the antibodies and by the number of RBC membrane determinants. The failure of maternal antibodies to opsonize the newborn's RBC and thus cause hemolytic disease is very likely due to the low avidity of antibodies and to the weak expression of blood group determinants on the membranes of these RBC.     

基于SuperMapIS.NET的地价动态监测系统及其WEB服务平台开发

从分析现有地价监测系统中存在的问题出发,提出基于SuperMapIS.NET进行地价动态监测系统及其WEB服务平台的设计与开发的原则、系统的功能设计、数据处理流程设计、数据组织结构设计,利用建立的地价监测系统对徐州市2011年第3季度的地价进行动态监测与基准地价更新工作,结果表明该系统可靠、稳定、适应性较强。     

Detection of Mycobacterium avium subsp paratuberculosis in formalin-fixed paraffin-embedded intestinal tissue by IS900 polymerase chain reaction

OBJECTIVES: To evaluate and compare methods for DNA extraction from formalin-fixed, paraffin-embedded tissues and methods for detection of Mycobacterium avium subsp paratuberculosis by IS900 PCR for confirmation of Johne's disease in ruminants. DESIGN: A laboratory study. PROCEDURE: Three methods of DNA extraction of differing complexity and two PCR protocols using different pairs of IS900 primers were compared. Sensitivity and specificity were assessed using samples from ruminants with and without histological evidence of Johne's disease. RESULTS: The simplest method of DNA extraction, which involved two cycles of boiling and freezing followed by centrifugation, gave more consistent results than two methods that required solvent extraction of paraffin, proteinase digestion and DNA purification. The sensitivity of detection of M avium subsp paratuberculosis in paraffin blocks stored for 1 to 6 years from 34 cases of Johne's disease in sheep, cattle and goats was 88% for a 229 bp IS900 PCR assay and 71% for a 413 bp assay, using the detection of acid-fast bacilli by Ziehl Neelsen staining of histological sections from the same blocks as the gold standard test. PCR results correlated with the abundance of acid-fast organisms in the tissues. No false positive reactions were detected. CONCLUSION: PCR for identification of M avium subsp paratuberculosis in formalin-fixed, paraffin-embedded intestinal tissues from ruminants is a rapid and useful method. A simple method of sample preparation is effective. Amplification of short fragments of IS900 is more effective than amplification of longer fragments.     

Pathogenic 'Bison-type' Mycobacterium avium subspecies paratuberculosis genotype characterized from riverine buffalo (Bubalus bubalis) in North India

Despite low per-animal productivity of ruminants in developing countries, Johne's disease has not been investigated in buffaloes, which are primarily found in these countries. This is due to lack of expertise, diagnostic kits and priority to production diseases like Johne's disease. Presence of pathogenic Mycobacterium avium subspecies paratuberculosis (Map) was investigated by screening of target tissues (mesenteric lymph nodes and large intestine) by culture and IS 900 PCR, in 50 sacrificed buffaloes. Indigenous ELISA kit originally developed for goats and sheep was standardized in buffaloes and used to estimate sero-presence of Map in 167 serum samples representing population of buffaloes in Agra region of North India. In culture, 48.0% buffaloes were positive from 50 tissues each from mesenteric lymph nodes (34.0%) and large intestine (36.0%). IS 900 PCR was standardized using specific primers (150 C and 921) and 229 bp-amplified product was characteristic for Map. Of the 25 mesenteric lymph nodes, 40.0% were positive in IS 900 PCR. Genomic DNA from Map cultures was successfully amplified from all the 24 isolates (100.0%). Map was further genotyped as 'Bison type' using IS 1311 PCR-REA. Culture of tissues showed high presence of Map in target tissues, despite high culling rate in buffalos in view of high demand of buffalo meat. Specific tissue-PCR provided rapid confirmation of Map infection in sacrificed buffaloes. In tissue-PCR, all the cultures were positive as compared to 40.0% detected directly from tissues. ELISA kit using indigenous protoplasmic antigen was highly sensitive as compared to commercial antigen in detecting Map infection therefore, could be used as 'Herd Screening Test' in buffaloes against Johne's disease. This pilot study first time reports a highly pathogenic 'Bison-type' genotype of M. avium subspecies paratuberculosis from the riverine buffaloes (Bubalus bubalis) of Agra region in North India.     

Histopathological classification of systemic Mycobacterium avium complex infections in slaughtered domestic pigs

The aim of this study was to classify the histopathological features of pigs infected with Mycobacterium avium complex (MAC). We used slaughtered pig organs systemically infected with MAC. The results showed granulomatous lesions which were observed predominantly in the digestive organs and regional lymph nodes rather than respiratory organs. The histological picture showed a wide range of granulomatous stages from exudative to fibrotic reactions to the MAC infection. Eosinophils and giant cells were characteristically observed in the exudative reactions. The histopathological type in primary focus tended to be maintained in the respective organs. Most strains with the same genotype showed pathogenicity for guinea pigs irrespective of the type of granuloma. Although these findings suggest that different stages of a granulomatous lesion originating from the same causative agent might influence histological patterns, other possibilities such as the hereditary background of the host, or the effects of viral infections should be considered.