湖南中外合作林业项目的特点与思考

中外合作林业项目对促进我国林业发展的作用日益明显,就湖南中外合作林业项目的特点进行了阐述,并与国内传统林业项目进行了比较,对湖南林业进一步的发展提出见解.     

Comparison of kinetic properties of a hydrophilic form of acetylcholinesterase purified from strains susceptible and resistant to carbamate and organophosphorus insecticides of green rice leafhopper (Nephotettix cincticeps Uhler)

A hydrophilic form of acetylcholinesterase (AChE) was purified from N-methyl carbamate susceptible (SA) and highly N-methyl carbamate-resistant (N3D) strains of the green rice leafhopper (GRLH), Nephotettix cincticeps Uhler. Both of purified AChE from SA and N3D strains displayed the highest activities toward acetylthiocholine (ATCh) at pH 8.5. In the SA strain, the optimum concentrations for ATCh, propionylthiocholine (PTCh), and butyrylthiocholine (BTCh) were about 1 × 10⁻³, 2.5 × 10⁻³, and 1 × 10⁻³ M, respectively. However, in the N3D strain, substrate inhibition was not identified for ATCh, PTCh, and BTCh to 1 × 10⁻² M. The K_m value in the SA strain was 51.1, 39.1, and 41.6 μM and that in the N3D strain was 91.8, 88.1, and 85.2 μM for ATCh, PTCh, and BTCh, respectively. The K_m value in the N3D strain indicated about 1.80-, 2.25-, and 2.05-fold lower affinity than that of the SA strain for ATCh, PTCh, and BTCh, respectively. The V_max value in the SA strain was 70.2, 30.5, and 4.6 U/mg protein and that in the N3D strain was 123.0, 27.0, and 14.5 U/mg protein for ATCh, PTCh, and BTCh, respectively. The V_max value in the N3D strain was 1.75- and 3.15-fold higher for ATCh and BTCh than that in the N3D strain. However, it was 1.13-fold lower for PTCh. The increased activity of AChE in the N3D strain is due to the qualitatively modified enzyme with a higher catalytic efficiency. The bimolecular rate constant (k_i) for propoxur was 27.1 × 10⁴ and 0.51 × 10⁴ M⁻¹ min⁻¹ in the SA and N3D strain and that for monocrotophos was 0.031 × 10⁴ and 2.0 × 10⁴ M⁻¹ min⁻¹ in the SA and N3D strain. AChE from the N3D strain was 53-fold less sensitive than SA strain to inhibition by propoxur. In contrast, AChE from the N3D strain was 65-fold more sensitive to inhibition by monocrotophos than AChE from the SA strain. This indicated negatively correlated cross-insensitivity of AChE to propoxur and monocrotophos.     

高辣椒素含量航天辣椒桂航一号的选育

桂航一号是红椒一号经过太空诱变处理后,单株选育3代、混合选育2代育成的高辣椒素含量辣椒新品种。该品种中晚熟,从定植至采收红椒约90d。株高90cm左右,植株开展度85cmx85cm左右。第一花着生13—14节。果实羊角形,纵径15～16cm,横径1．3cm,肉厚0．19cm,2—3心室。果肩平或斜,果顶锐尖,果面光滑,果皮薄,果形较直,青果深绿色,生物学成熟果鲜红色,平均单果质量12～13g,最大单果质量15g。果实每100g鲜质量维生素C含量87．9mg,辣椒素含量104mg／kg,干物质含量8．56％。其中辣椒素含量比红椒一号（5．55mg／kg）提高了近20倍,解决了红椒一号作为辣椒酱原料辣度不够的缺点。667m2产量3942．1奴,较红椒一号增产15．8％,达极显著水平。植株外被茸毛,较耐炭疽病、疫病、病毒病,抗高温,耐湿能力强,适于嗜辣地区作鲜食、加工盐渍、酱制或干制栽培。     

聊城古城景观保护及绿地生态规划研究

基于聊城古城景观结构特色及其建设中存在的问题,依据景观生态学的原理和方法,提出古城景观保护方案并进行绿地系统生态规划。     

佛山市文华公园总体规划

佛山市文华公园是佛山市禅城区中心组团城市景观轴线的重要节点。整个公园采取开放式设计,力图建设成为一个高品质的具有浓郁岭南文化意蕴的“城市绿色客厅”。     

广州旧城区绿地建设对策研究

通过分析城市旧城区绿地建设普遍存在的困难,在建设策略上,提出规划建绿、文化建绿、见缝插绿、立体增绿、临时建绿、拆危建绿、绿地提升、保存大树等针对旧城区绿地建设的对策。     

水稻超高产强化栽培技术体系

介绍了水稻超高产强化栽培技术体系。该技术体系采取育种与栽培相结合、生产技术集成与示范推广相结合的技术路线,以节水型优质超级稻新品种为基础,以农业水稻超高产强化栽培为主攻方向,实现水稻高产为目标。     

将农民工纳入城市住房保障体系的几点思考

逐步将农民工纳入城镇住房保障体系中,是城镇化进程的必然趋势,也是全面贯彻落实科学发展观,构建和谐社会的必然要求。但应充分考虑到农民工自身的特点和各地的实际情况,要科学纳入而不应盲目扩容;同时还应着力研究进城定居农民工享受城市住房政策和农村宅基地政策的衔接,统筹城乡资源。     

我国蜂蜜出口中的绿色壁垒影响剖析

近年来我国蜂蜜出口屡屡遭遇发达国家设置的绿色壁垒。对此应该冷静客观审视：正当合理的绿色壁垒对我国蜂蜜出口有着积极的导向作用。从长期来看,这种导向作用必定会促使我国蜂蜜出口竞争力大大提升;但是短期内绿色壁垒对我国蜂蜜出口实际造成一定消极影响。为此,应该采取积极主动的生产与贸易对策。     

脑心肌炎增强型绿色荧光蛋白嵌合病毒的构建

携带增强型绿色荧光蛋白(Enhanced green fluorescent protein, EGFP)的脑心肌炎(Encephalomyocarditis virus, EMCV)嵌合病毒是研究该病毒体内外生物学特性的有力工具。因此,本研究基于前期构建的巨细胞病毒(Cytomegalovirus, CMV)感染性克隆,在EMCV基因组2A蛋白序列之后插入EGFP基因片段,并将重组质粒转染BHK-21细胞,获得携带EGFP的嵌合病毒。通过荧光定量PCR(real-time PCR)、间接免疫荧光(Indirect immunofluorescence assay, IFA)及半数组织细胞感染量测定(Median tissue culture infective dose, TCID₅₀)等方法对拯救病毒的基因组复制、蛋白表达及病毒粒子的感染性进行测定,结果证实嵌合病毒能够在BHK-21细胞上成功表达EGFP并产生完整的病毒粒子。然而,相较于野生型亲本拯救病毒,嵌合病毒在BHK-21细胞上的复制能力降低,并且在连续传代后逐渐失去绿色荧光信号,表明嵌合病毒中EGFP...