排序方式: 共有26条查询结果,搜索用时 78 毫秒
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以穿龙薯蓣的丛生芽为材料,对根的诱导及快速繁殖进行了研究,并利用反相高效液相色谱技术对根中薯蓣皂苷元含量进行了测定,结果如下:不添加任何激素的1/2MS培养基是诱导生根的最佳培养基,生根率最高达76.7%;MS+0.2 mg/L NAA+0.5 mg/L IBA+0.2-0.5 mg/L PP333为根增殖的最佳培养基,60 d时增殖倍数超过93;快繁过程中根的生长形态发生变化,不同类型的根中薯蓣皂苷元含量差异不显著,平均为0.21% 相似文献
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PPARγ mediates effects of diosgenin on proliferation and apoptosis in human glioblastoma U87MG cells
AIM:To investigate the effect of diosgenin (Dio) on the proliferation, apoptosis and expression of peroxisome proliferator-activated receptor γ (PPARγ) in human glioblastoma U87MG cells and its possible mechanism. METHODS:Human astrocytes (HA) and U87MG cells were cultured in vitro and treated with Dio (0, 10, 20, 30, 40 and 50 μmol/L) and GW9662 (5 μmol/L) for 48 h, and then the cell viability was detected by CCK-8 assay. Cell colony formation assay was used to assess the proliferation potential. Flow cytometry was used to analyze the cell cycle distribution and apoptosis. The mRNA expression level of PPARγ was measured by RT-PCR. Western blot was used to determine the protein levels of PPARγ, cyclin D1, cyclin E1, Bcl-2 and Bax. RESULTS:Dio had no significant influence on the viabi-lity of HA (P>0.05). However, Dio remarkably reduced the viability of U87MG cells in a dose-dependent manner (P<0.05) with IC50 of 24.31 μmol/L. Meanwhile, Dio remarkably diminished colony formation ability (P<0.05), induced G0/G1 phase arrest of the cell cycle and apoptosis (P<0.05), up-regulated the expression of PPARγ at mRNA and protein levels, increased the protein level of Bax (P<0.05), and down-regulated the protein levels of cyclin D1, cyclin E1 and Bcl-2 (P<0.05) in a dose-dependent manner. However, these effects induced by Dio were inhibited by GW9662 (P<0.05), a specific inhibitor of PPARγ. CONCLUSION:Dio may inhibit proliferation and induce apoptosis in human glioblastoma U87MG cells most likely via up-regulating the expression of PPARγ, and then down-regulating the protein levels of cyclin D1, cyclin E1 and Bcl-2, and up-regulating the protein level of Bax. 相似文献
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[目的]明确钙对盾叶薯蓣产量和皂素含量的影响。[方法]用0、20、40、80、120mg/kg钙分别处理盾叶薯蓣的实生苗,测定盾叶薯蓣产量、皂素含量、叶片叶绿体Ca2+-ATPase的活性和土壤中有效钙含量。[结果]随钙肥用量的升高,盾叶薯蓣产量、皂素含量均呈先上升后下降的趋势。钙肥用量为80mg/kg时,单株平均产量最高,为38.967g/株;有效钙含量最低(10.721mg/100g);叶绿体Ca2+-ATPase活性最高[26.740μmol/(mg.h)]。钙肥用量为40mg/kg时,皂素含量最高,为2.56%。[结论]在一定的范围内,钙肥可明显提高盾叶薯蓣产量和皂素产量。Ca2+通过影响叶绿体Ca2+-ATPase的活性而影响Ca2+吸收,进而影响盾叶薯蓣产量,但是Ca2+-ATPase的活性变化与皂素含量未显示出相关性。 相似文献
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[目的]测定怀山药及其零余子中薯蓣皂苷元成分的含量。[方法]以齐墩果酸为标准对照品,浓度5%香草醛-冰醋酸和高氯酸混合液为显色剂,用紫外可见分光光度计在波长547 nm处对怀山药及其零余子的皂苷成分进行含量测定。[结果]怀山药中薯蓣皂苷元含量为0.016 4%,怀山药零余子中薯蓣皂苷元含量为0.021 3%,平均加标回收率为98.46%,RSD为1.36%;零余子中薯蓣皂苷元含量略高于怀山药。[结论]该方法简便、易行、准确度高,可用于怀山药零余子中薯蓣皂苷元成分的含量测定。 相似文献
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HPLC测定不同生长年限滇重楼中薯蓣皂苷元含量 总被引:4,自引:1,他引:3
[目的]探讨滇重楼(RHIZOMA POLYGONATI)中薯蓣皂苷元的含量与生长年限的关系,为确定其最佳采收年限提供参考依据。[方法]采用HPLC测定不同生长年限滇重楼中薯蓣皂苷元的含量。色谱柱为Agilent zorbax SB-C18柱(4.6mm×150.0mm,5μm),流动相为乙腈-水(90∶10,V/V),流速为1.0 ml/min,检测波长为203 nm,柱温为35℃。[结果]不同生长年限的滇重楼中薯蓣皂苷元含量明显不同。其中,3、4年生滇重楼中薯蓣皂苷元含量较低;5、6年生滇重楼中薯蓣皂苷元含量明显升高,但6年生的含量略低于5年生;7年生滇重楼中薯蓣皂苷元含量骤升,且升幅最大。[结论]不同生长年限滇重楼中薯蓣皂苷元的含量不同,在实际应用时应根据不同需要适时采收。 相似文献
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酵母菌生物接触氧化工艺处理皂素生产废水研究 总被引:2,自引:0,他引:2
以酵母菌作为特定微生物,采用生物接触氧化工艺处理皂素生产废水,对其工艺参数进行了优选 研究。结果表明,生物接触氧化工艺中以酵母菌为优势菌群,当温度为28-30 C,进水化学需氧量(COD)为2 710 -9 200 mg/L,pH值为4.5-5.5,溶解氧为4.5 mg/L,水力停留时间(HRT)为12 h时,COD去除率超过70%, 反应出水回收的酵母干菌稳定在8.3g/L以上,且产量随进水有机物浓度升高而上升。 相似文献
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