‘丰香’草莓叶片高效再生体系的建立

 以草莓主栽品种丰香( Fragaria ×ananassa Duch ‘Toyonoka’) 叶片为外植体, 研究了影响组织培养的多个因素, 建立了高效再生体系。结果表明: MS + TDZ 1.5 mg·L ^{- 1} + IBA 0.4 mg·L ^{- 1}培养基最适于不定芽分化。不同滤光膜(绿膜、红膜、蓝膜、黄膜) 对草莓不定芽的分化具显著效应, 绿膜和红膜对芽的分化有明显促进作用, 不定芽再生率达95%以上, 平均每个外植体再生芽数在25个以上; 而蓝膜和黄膜则不利于芽的分化。不同滤光膜光谱差异主要集中在300～700 nm, 红、绿膜在该波段光强较弱,而黄、蓝膜和荧光灯较强。暗处理4周比1、2、3周更有利于提高叶片的不定芽再生率, 此后转到光下培养, 获得的不定芽再生率可达100%。     

荔枝冷害过程中果皮色泽、花色素苷和类黄酮含量的变化

 研究了‘糯米糍’荔枝果实在3 ℃和0 ℃条件下果皮的色泽和花色素苷及类黄酮含量的变化。结果表明, 果实在0 ℃下14 d 时褐变指数迅速增大, 21 d 时已遭受不可逆的冷害。在冷藏过程中, 果皮的色泽指标a、L、C 值及花色素苷、类黄酮、总酚含量均随贮藏时间的延长而下降, 0 ℃下比3 ℃下降快。0 ℃下14～21 d , a、L、C 值分别下降了27.95 %、33.58 %和58.47 % , 而21 d 时果皮花色素苷、类黄酮和总酚含量依次为采收时的18.08 %、35.99 %和41.64 %。说明冷害加速了荔枝果皮花色素苷的降解及类黄酮和总酚的变化, 促进了褐变进程的发生。果皮a、L、C 值与褐变指数、花色素苷含量呈显著相关性( P < 0.05) ,采用自动测色色度计可真实地反映果皮花色素苷含量和褐变程度。     

Seasonal variation in pigmentation and anthocyanidin phenetics in commercial Eustoma flowers

The seasonal change in petal color and pigmentation of 29 commercial Eustoma cultivars was studied. The flowers are basically divided into four groups according to the major anthocyanidin phenotype in association with petal coloration, i.e., delphinidin (Dp)-based (purple flower), cyanidin (Cy)-based (reddish purple flower), pelargonidin (Pg)-based (pink flower), and none (white flower) groups. The constitution of petal anthocyanidins was not changed by forcing treatment in most of the flowers. Lightness (L^*) and chroma (C^*, color saturation) showed a change along with the increase/decrease of hue angle difference (ΔH^*), thus simultaneously the chromatic tonalities tended to move to redder and bluer, respectively. Floral pigment clustering described two flower groups in a dendrogram, based on anthocyanidin constitutions as phenetic markers, which are apparently the Dp- and Pg-based phenotypes of anthocyanidin syntheses. The Cy-based flowers made a subcluster with the Pg-based flowers, indicating a close relationship in the biosynthesis of the two anthocyanidins, and suggesting the Dp- and Pg-syntheses complement one another.     

离心泵整体叶轮流道数控加工误差的仿真

对数控加工所获得的叶轮流道的加工误差进行仿真。首先对刀位轨迹线进行归一化处理,以此计算出脊棱高度曲线,再采用线性插值的方法得到曲面上采样点的加工误差。由于自由曲面加工中的刀位点比较密集,本文利用彩色云图将加工误差可视化,完善了对数控加工结果的计算机几何仿真。该算法已在自行研制的叶轮CAM系统中得到验证,并且与实际加工结果一致。     

Effects of fruit bagging on coloring and related physiology,and qualities of red Chinese sand pears during fruit maturation

Red Chinese sand pears (Pyrus pyrifolia Nakai) are particular to China. In order to determine the effects of fruit bagging treatments (including bag types, bag removal patterns and dates) on fruit qualities and to understand the mechanism of coloring of red Chinese sand pears, two experiments were carried out. In the first experiment, fruit of ‘Meirensu’ were firstly covered by light-impermeable paper bags with different levels of light permeable liners during their early development stage, then, the whole bag were/not removed or only the outer layer of bags were removed 3, 2 or 1 weeks before harvest. Thus, the fruit were/not totally re-exposed or were under different levels of sunlight transmission (80.31% or 34.71%). Non-bagged fruit were used as the control. Bagging treatments significantly affected the concentration of anthocyanin and the visual qualities of pear fruit. Compared to control, fruit re-exposed totally for 2 or 3 weeks accumulated the largest amount of anthocyanin and fruit receiving 80.31% and 34.71% of sunlight for 1–3 weeks could synthesize a little anthocyanin, indicating that high light intensity is imperative for coloring in red Chinese sand pears. Bagging treatments did not affect contents of total soluble sugars, but decreased organic acids contents in fruit. In the second experiment, fruit of ‘Meirensu’ and ‘Yunhongli No. 1’ were covered with only one type of light-impermeable bag during the early development stage and totally re-exposed after the bag removal 15 days before harvest. Fruit were then collected at different intervals to trace the time-course of coloring, and related physiology and inner qualities. With increasing time after the bag removal, the concentration of chlorophyll, carotenoid, flavonoid and total phenols changed little, but the concentration of anthocyanin accumulated extremely fast within 10 days after the bag removal in both cultivars and thereafter kept constant. ‘Yunhongli No. 1’ had higher anthocyanin contents and lower hue angle than ‘Meirensu’, indicating a higher potential of anthocyanin synthesis. After the bag removal, the sucrose contents and PAL activities increased gradually and correlation analysis revealed that they were highly correlated with anthocyanin accumulation in two cultivars. This study suggests that anthocyanin biosynthesis in red Chinese sand pears is a highly light dependent process and modified by genotypes. Based on the current results, in order to obtain red Chinese sand pear fruit with attractive appearance and good inner qualities, fruit must be covered with light-impermeable bags at the early stage of fruit development and the bag should be removed totally at least 10 days before harvest.     

驾驶员的视觉特性对道路交通安全的影响研究

为了更好地提高道路交通安全管理水平、减少交通事故,本文对关系驾驶员安全行车的视觉特性如视力、视野、立体视觉、色视觉、视觉适应等因素结合交通事故统计资料进行了分析,阐述了驾驶员的视觉特性对道路交通安全的影响。     

云南省地方玉米种质资源的类型、品种及其子粒颜色多样性分析

对云南省地方玉米种质资源类型、品种(遗传)及子粒颜色的整理和分析,表明云南省玉米地方种质资源共有7个类型.类型多样,分布广泛,但不同类型的生态分布区和品种及其子粒色泽多样性存在较大差异.硬粒型品种及其子粒色泽多样性指数最大,品种数最多,占地方种质总数的58.2%;其次是半马齿型和糯质型品种,子粒颜色以黄、白色为主,分别占品种总数的43.3%和42.8%,其他颜色较少.糯质型品种主要集中分布于滇南和滇中两大生态区,存在不同的品种类型和丰富的种质资源,以及水平分布的区域性和立体分布的生态特点.硬粒型玉米品种的多样性与云南省的耕地成分、分布状况、人们种植和利用玉米的目的及用途密切相关.糯质玉米的丰富性、多样性和生态分布特点主要与云南省各民族人民的民族饮食文化密切相关。     

提高名优绿茶色泽的加工技术措施

本文分析了名优绿茶不绿的原因,提出了改进加工技术,确保名优绿茶色泽的技术措施.     

热处理对马尾松蓝变材颜色的影响

热处理选取了5个温度(170、185、200、215、230℃)和4个时间(2、4、6、8 h)。在热处理前后分别测量蓝变点与未蓝变点的颜色,分析了蓝变材颜色的均匀性,对比了蓝变点与未蓝变点的颜色差异和变化规律。实验结果表明:热处理后,蓝变材颜色参数的标准差逐渐降低,蓝变点与未蓝变点明度差、黄蓝色品指数差减小,其色差可以从17.57降低到小于1。均值比较结果显示,经过200℃以下的热处理,蓝变点与未蓝变点间的颜色参数仍然具有显著性差异。为了使蓝变色斑达到肉眼不容易识别的程度,最低的热处理条件为200℃下处理6 h。