Field performance and molecular diversification of lemon selections

Lemon (Citrus limon (L.) Burm. f.) is one of the most important Citrus fruit for Turkey because of its great amount of production and export. It has been cultivated for a long time in Turkey, and therefore variations for agronomical traits are likely among cultivated lemons due to bud mutations and, hybridizations. The objectives of this study were to determine variations for some selected agronomical traits and genetic markers among 12 new lemons derived from selections. Tree growth, yield, fruit quality, and molecular diversification of these clones were determined. After four years of evaluation, ‘Kutdiken’ M-51 indicated the highest canopy volume. For yield per tree, the best clone was ‘Kutdiken’ M-51. After five years of evaluation, ‘Kibris’ M-54 had the highest fruit weight and acidity. ‘Italian Memeli’ M-56 contained the lowest seed number and the highest total soluble solids. Molecular analysis, as assessed with 22 random amplification of polymorphic DNA (RAPD) and 11 inter simple sequence repeats (ISSR) primers, indicated that seven of twelve clones were separated with RAPD markers, whereas four were distinguished with ISSR markers. Combined analysis of RAPD and ISSR data detected that similarity values among the lemons clones were between 0.97 and 1.00. It can be concluded that variations in orchards are abundant and mainly due to mutations.     

Sap flow in ‘Hass’ avocado trees on two clonal rootstocks in relation to xylem anatomy

The rates of sap flow and xylem vessel features were studied in two-year-old nongrafted and grafted avocado (Persea americana Mill.) trees. Daily sap flow rates were measured with heat and balance stem gauges in clonal Duke 7 (D7) and Toro Canyon (TC) trees and ‘Hass’ clonal scions grafted onto clonal D7 (H/D7) and TC (H/TC) rootstocks. Vessel features as size, number and total vessel area were determined histologically in the stem of the scion and rootstock and the roots of the grafted trees. Significant differences in the sap flow rate were found among the rootstocks, where D7 had a 29% higher sap flow rate than did TC (grafted and nongrafted trees). There were no differences among xylem vessel features in the stems of any of the varieties. However in the roots, D7 had wider and fewer vessels then TC do. Also, D7 had a 19% higher total vessel area than TC. These results suggest that the differences in water consumption of ‘Hass’ on different rootstocks may be associated with differences in the efficiency of the roots to absorb water across conductive tissue which may be linked to differences in the area of xylem vessels in the root.     

无性系植物种群生态学研究进展

在回顾无性系无种群动态遗传后果、生理整合作用以及无性系生长格局研究现状的基础上,指出了研究中存在的问题,并对无性系植物种群生态学的研究前景进行了展望。     

白三叶(Trifolium repens L)无性系植物分株间的生理整合作用

研究了白三叶无性系植物种群，在相互连接的分株之间生理上相互依存的程度。结果显示子代在生理上取决于母株资源的转移，这一依赖性随时间而下降。相互连接的分株，一部分被遮荫至１０％，而照光分株光合速率明显增加。光被限制期间，分株将重新整合，遮荫分株无性系中由其它分株提供同化物。在子代匍匐茎数目和长度上有一个权衡。当资源受限制时，产生极少较长的子代匍匐茎，结果提高了子代能够占据更有利条件的可能性。在分株之间，生理整合作用降低了子代建立所面临的危险，使同源分株在资源的可利用能力上整合到一个局部的异质环境，增加同源分株间的竞争能力，缓冲所面对的局部微生境的选择。     

林木无性繁殖及其在林业生产中的应用进展

在对无性繁殖的特点进行总结的基础上，综述了无性繁殖在无性系林业、林木遗传改良、林木复幼等方面应用的最新进展。     

毛白杨,河北杨多代循环繁殖方法的研究

从1983年起,对毛白杨、河北杨的扦插繁殖育苗及其机理进行了研究,研究出“多代循环繁殖新方法”,无需任何处理,也无需地膜覆盖,使大田育苗成活率稳定在80％左右;同时研究出快速、简便、繁殖系数高的优树根萌条培育新技术,为毛白杨、河北杨优树的无性系测定、无性系育种和无性系造林创造了条件。     

乌桕优良无性系评选及可配性研究

对收集的２０个乌桕无性系的生物学特性，主要经济性状进行了观测和比较，经多年测产，评选出８个高产无性系，并进行了可配性试验，用无性系造林，宜选择葡萄桕（类型）混合种植。     

Variation in juvenile traits of natural black poplar (Populus nigra L.) clones in Turkey

Natural black poplar (Populus nigra L.) clones sampled from river courses were tested in the arid southeast region of Turkey, using a randomized complete block field design with four replications. Clones were laid out in row plots of eight ramets each. Height and apical dominance were assessed at age one year; diameter, survival, bole straightness and branchiness were measured at age two years. Clones differed significantly in survival, growth and quality traits. The results showed that promising clones exist in natural populations. Two commercial clones out of four did not grow as fast as the top new selection clones. For bole straightness, three commercial clones had significantly lower grading scores than the top best 14 clones. Principal components analysis indicated that growth, apical dominance and branching are the most important traits distinguishing black poplar clones. Diameter had a moderate correlation (0.34) with bole straightness. Relationships between geographic variables (elevation, latitude and longitude) with growth and stem quality traits were weak. Considerable genetic variation was observed among clones for all the traits. Genetic differences among the clones accounted for 27% (survival, bole straightness) to 39% (height) of the total variance. Broad-sense individual heritability ranged from 0.27 (survival) to 0.37 (apical dominance). Clonal mean heritabilities were higher than individual heritabilities and ranged from 0.60 (survival) to 0.82 (diameter), implying considerable gain could be realized via selective improvement methods.     

山梨优良品系的选择

2012~2013年,对山梨园中不同品系山梨进行了果实大小、单株结果产量、石细胞含量、出汁率、可溶性固形物含量、可溶性糖含量等指标的测定和评价。结果表明:初选的12个品系22株山梨个体中,有8个品系12株山梨个体在1个或多个果实性状指标上表现比较突出。具有较好鲜食品质的有YS10-5、YS15-1、YS15-2、YS11-2;具有较好加工品质的有YS6-8、YS6-9、YS10-5、YS15-1和YS15-2;具有较高产量的有YS6-9、YS11-5、YS10-5、YS15-1、YS9-2、YS13-6、YS13-4、YS14-2、YS14-5、YS8-4和YS7-6;具有果个大且品质好的有YS10-5、YS11-2、YS11-5和YS15-1。通过模糊数学隶属函数对12个品系22株山梨个体综合评判,有6个优良品系9株优良单株果实各项指标隶属函数均值较大,从大到小依次为YS10-5、YS11YS11-5、YS9-2、YS15-1、YS14-5、YS11-2、YS6-9、YS10-2和YS15-2,其隶属函数均值范围为0.5068-0.8335。     

Multiple sampling and discriminatory fingerprinting reveals clonally complex and compartmentalized infections by M. bovis in cattle

The combination of new genotyping tools and a more exhaustive sampling policy in the analysis of infection by Mycobacterium tuberculosis has shown that infection by this pathogen is more complex than initially expected. Mixed infections, coexistence of clonal variants from a parental strain, and compartmentalized infections are all different modalities of this clonal complexity. Until recently, genotyping of Mycobacterium bovis in animal populations was based on spoligotyping and analysis of a single isolate per infection; therefore, clonal complexity is probably underdetected. We used multiple sampling combined with highly discriminatory MIRU-VNTR to study compartmentalized infections by M. bovis in a low-tuberculosis prevalence setting. We spoligotyped the M. bovis isolates from two or more anatomic locations sampled from 55 animals on 39 independent farms. Compartmentalized infections, with two different strains infecting independent lymph nodes in the same animal, were found in six cases (10.9%). MIRU-VNTR analysis confirmed that the compartmentalization was strict and that only one strain was present in each infected node. MIRU-VNTR analysis of additional infected animals on one of the farms confirmed that the compartmentalized infection was a consequence of superinfection, since the two strains were independently infecting other animals. This same analysis revealed the emergence of a microevolved clonal variant in one of the lymph nodes of the compartmentalized animal. Clonal complexity must also be taken into consideration in M. bovis infection, even in low-prevalence settings, and analyses must be adapted to detect it and increase the accuracy of molecular epidemiology studies.