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[目的]研究大蒜套种石榴控制石榴枯萎病的可能性。[方法]在实验室条件下,采用不同浓度的0.2倍MS培养液和蒸馏水培养的大蒜根系分泌物,测定其对枯萎病菌和枯草芽孢杆菌生长的影响。[结果]MS培养液和蒸馏水培养的大蒜根系分泌物均不能有效抑制枯萎病菌菌丝的生长。而同样2种方式培养的大蒜根系分泌物却对枯草芽孢杆菌的增殖具有较强的促进作用,且蒸馏水培养大蒜根系分泌物效果要优于MS培养液培养的根系分泌物。[结论]石榴园套种大蒜结合枯草芽孢杆菌施用有望作为控制石榴枯萎病蔓延的一种方式。 相似文献
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The pathogenicity and growth of six cultures of the bark-beetle-associated blue-stain fungus Ceratocystis polonica were compared. Four were subcultures, established at different times, of the same isolate (80–53/7), and at least one of these was suspected to have lost its pathogenicity to Norway spruce ( Picea abies ) during subculturing. Two other cultures (93–208/115 and 94–169/13) were pathogenic. The pathogenicity of all cultures to Norway spruce was compared in three inoculation experiments, using (i) massive inoculation (≈144 inoculations per tree), (ii) low-density inoculation (14 inoculations per tree) of larger trees and (iii) single inoculation of 2-year-old seedlings. Fungal growth rates were tested in vitro on malt agar, and on malt agar under oxygen-deficient conditions. Isolates 93–208/115 and 94–169/13 and one of the subcultures of isolate 80–53/7 were pathogenic in all three inoculation experiments. Two other subcultures were nonpathogenic, while the last subculture was intermediate. The pathogenic cultures had higher growth rates on malt agar than the nonpathogenic and intermediate cultures, and they also grew better under oxygen-deficient conditions. These results demonstrate that blue-stain fungi may lose their pathogenicity after serial vegetative transfers on artificial substrates. The results are also consistent with the hypothesis that rapid growth and the ability to grow in oxygen-poor environments are important pathogenicity factors in blue-stain fungi. 相似文献
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C. El Modafar A. Clérivet A. Vigouroux J. J. Macheix 《European journal of plant pathology / European Foundation for Plant Pathology》1995,101(5):503-509
Inoculation of leaves of resistantPlatanus occidentalis and susceptiblePlatanus acerifolia leaves withCeratocystis fimbriata f. sp.platani, the canker stain disease agent, induced foliar necrosis and biosynthesis of two phytoalexins, scopoletin and umbelliferone. Foliar symptoms keep localized and accumulation of coumarin phytoalexins was rapid for incompatible interactions. Necrosis spread widely and accumulation of these phenolic compounds was much later and lower for compatible interactions. The differential response could be used in a genetic improvement program for resistance against canker stain. 相似文献
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M. D. Pintore L. Ceballos B. Iulini L. Tomassone A. Pautasso D. Corbellini F. Rizzo M. L. Mandola M. Bardelli S. Peletto P. L. Acutis A. Mannelli C. Casalone 《Zoonoses and public health》2015,62(5):365-374
Following reports of human cases of Lyme borreliosis from the Ossola Valley, a mountainous area of Piemonte, north‐western Italy, the abundance and altitudinal distribution of ticks, and infection of these vectors with Borrelia burgdorferi sensu lato were evaluated. A total of 1662 host‐seeking Ixodes ricinus were collected by dragging from April to September 2011 at locations between 400 and 1450 m above sea level. Additional 104 I. ricinus were collected from 35 hunted wild animals (4 chamois, 8 roe deer, 23 red deer). Tick density, expressed as the number of ticks per 100 m2, resulted highly variable among different areas, ranging from 0 to 105 larvae and from 0 to 22 nymphs. A sample of 352 ticks (327 from dragging and 25 from wild animals) was screened by a PCR assay targeting a fragment of the 16S rRNA gene of B. burgdorferi s.l. Positive samples were confirmed with a PCR assay specific for the 5S‐23S rRNA intergenic spacer region and sequenced. Four genospecies were found: B. afzelii (prevalence 4.0%), B. lusitaniae (4.0%), B. garinii (1.5%) and B. valaisiana (0.3%). Phylogenetic analysis based on the ospC gene showed that most of the Borrelia strains from pathogenic genospecies had the potential for human infection and for invasion of secondary body sites. 相似文献
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Background
Lyme disease is commonly diagnosed in humans in Latvia, but up to date no studies have been performed to investigate its prevalence in dogs. The aim of this study was to evaluate if seroprevalence against B. burgdorferi sensu lato (B. burgdorferi s.l.) and co-expression of antibodies against B.burgdorferi s.l. and A. phagocytophilum is higher in dogs with clinical suspicion of tick-borne diseases compared to healthy dogs.Findings
Venous blood was taken from healthy dogs (n=441) and dogs suspected to have borreliosis and/ or canine granulocytic anaplasmosis (n=29). The presence of antibodies was detected with SNAP 4Dx test (IDEXX, Westbrook, Maine, USA). The seroprevalence against B. burgdorferi s.l. in healthy dogs was 2.49% (11/441) and 36% (4/11) of seropositive dogs had antibodies against both of investigated bacteria. None of the dogs in sick dog group had detectable antibodies against B. burgdorferi s.l.Conclusions
We conclude that seroprevalence to B. burgdorferi s.l. in dogs in Latvia is low and that dogs with suspicion of tick-borne disease do not have higher B. burgdorferi s.l. seroprevalence than healthy dogs. Dogs that express antibodies against B. burgdorferi s.l. frequently co-express antibodies against A. phagocytophilum. 相似文献10.
Ceratocystis albofundus is a recently described pathogen infectingAcacia mearnsii in South Africa, and it causes a disease known as Ceratocystis wilt. Symptoms of the disease include die-back, gummosis and
wilting of infected trees. In order to select trees tolerant to this fungus, susceptibility tests were conducted on trees
representing fourteen families ofA. mearnsii. A virulent isolate ofC. albofundus was selected and inoculated into the stems of twelve-month-old plants in a plantation. Lesion lengths, in the bark, and disease
development were assessed after 6 weeks. All fourteen families ofA. mearnsii were susceptible to infection byC. albofundus. Considerable variation was, however, noticed between individual trees within the same family and the incorporation of disease
tolerant trees into breeding programmes is proposed. 相似文献