高职食品与药品类专业“基础化学”教学改革与实践

“基础化学”理论和实验教学质量的“高与低”在一定程度上直接关乎高职院校与化学相近专业人才培养的“成与败”。以教学改革为切入点,在“基础化学”教材建设和系列微课的制作及应用2个维度上,探讨提升“基础化学”课程教学质量的具体措施。     

基于VBA的云南省基层林业工作站信息管理系统的设计与构建

为全面掌握全省各级林业站管理机构、各县(市、区)基层林业工作站的基本情况,基于云南省基层林业工作站情况调查的要求,结合乡镇林业工作站工程建设标准需要,以林业工作站调查信息数据为基础,利用VBA语言在Access 2007下开发具有形象直观的统计图功能、智能查询功能、数据导出至电子表格模板等功能特点的云南省基层林业工作站信息管理系统,文中阐述了系统开发方案、开发环境及系统主要功能。     

都兰县基本草原划定试点工作及做法

根据《农牧厅关于基本草原划定试点工作实施方案》的要求,都兰县2009～2010年开展基本草原划定试点工作,完成了野外调查、核实,内业整理、制图,政府公告、基本草原保护标志设立等工作。对试点工作加以总结,以期对全省基本草原划定工作提供借鉴。     

大别山区域生态农业建设基本指标及实践研究——以湖北省黄冈市为例

以黄冈市为例,分析了大别山区域生态农业建设的基本指标要求,以及落实大别山区域生态农业建设基本指标必须采取的具体措施.     

miR-122靶向调控鸡BZW2基因的表达

miR-122(MicroRNA-122)是在肝脏中高表达的miRNA,在肝脏的生长发育和脂类代谢等过程发挥重要作用,BZW2 (basic leucine zipper and W2 domains 2)主要参与蛋白质合成代谢,本研究目的是确定BZW2是否为miR-122调控的靶基因.通过生物信息学预测miR-122的靶基因,并分析BZW2的3-UTR区域与miR-122种子区的配对情况,再用双荧光素酶报告系统和突变实验证明miR-122作用于BZW2的3'UTR.用荧光定量PCR检测转染miRNA-122mimic的鸡(Gallus gallus)肝癌细胞系(leghorn hepatocellar,LMH)细胞和转染LNA-antimiR-122的鸡原代肝细胞中BZW2的表达.鸡BZW2的3'-UTR区域与miR-122种子区互补配对,荧光素酶报告基因和突变实验分析表明,miR-122能够通过与BZW23'-UTR区结合抑制基因的表达;将miR-122在LMH细胞中过表达后,发现BZW2 mRNA表达水平显著下降;利用LNA-antimiR-122抑制鸡肝脏原代细胞中的miR-122后,BZW2 mRNA表达水平呈显著性上升.结果证明BZW2是miR-122的靶基因,其在mRNA水平上受到miR-122的负性调控,本研究为揭示miR-122在鸡肝脏中的广泛的功能提供了理论依据.     

Effects of bFGF on brain edema,nerve function injury and autophagy related protein in rats with traumatic brain injury

AIM:To study the effects of basic fibroblast growth factor (bFGF) on brain edema, nerve function damage and autophagy related proteins in rats with head injury. METHODS:The rat model of craniocerebral injury (CI) was constructed. The rats were divided into control group, CI group, and low-, middle-and high-dose bFGF groups (n=10). The CI model was established in CI group, while the rats in control group were not given epidural impact. The rats in low-dose, middle-dose and high-dose bFGF groups were given bFGF at 2, 4 and 6 μg, respectively, by intraperitoneal injection after 30 min. The neurological function in the rats was evaluated by improved neurological function scoring. The rat brain tissues were taken, and the water content was detected. The levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1β in the brain tissue were measured by ELISA. The malondialdehyde (MDA) content was analyzed by thiobarbituric acid method. The activity of superoxide dismutase (SOD) was examined by WST-8 assay. The glutathine peroxidase (GSH-Px) activity was detected by colorimetric method. The protein levels of autophagy related proteins LC3-Ⅱ and beclin-1 in the brain tissues were determined by Western blot. RESULTS:The neurological function score was increased significantly of the rats in CI group. The rat model of craniocerebral injury was successfully constructed. Neurological function scores in the rats in low-dose, middle-dose and high-dose bFGF groups were reduced, the water content of the brain tissue was also reduced (P<0.05). The levels of TNF-α, IL-6 and IL-1 β were decreased in the brain tissues (P<0.05), the content of MDA was declined (P<0.05), the activities of SOD and GSH-Px were increased (P<0.05), the protein levels of LC3-Ⅱ and beclin-1 were decreased, compared with the untreated rats in CI group (P<0.05). CONCLUSION:bFGF improves the nerve function of the rats with craniocerebral injury, reduces the water content of the brain tissue, reduces the expression of autophagic protein LC3-Ⅱ and beclin-1.The mechanism is related to the inhibition of inflammatory reaction and oxidative damage.     

关于计算机基础课实行无纸化考试的几点思考

非计算机专业计算机基础课程面向所有非计算机专业学生,考试覆盖范围广、规模大,实行无纸化考试是一种必然趋势。该文分析了高校计算机基础课程无纸化考试的优越性,提出了无纸化考试系统的要求,并对所存在的问题进行了分析。     

森林工程专业的机器人技术基础课程改革研究

机器人技术基础是东北林业大学工程技术学院森林工程专业的选修课,是机械、电子、控制、检测、通信和计算机相关知识的有机融合。笔者结合森林工程专业的办学定位,分析该课程在森林工程教学中的作用和现状,从教学内容、教学方法、课程学习评价等方面,提出了机器人技术基础的课程改革办法。这些改革办法的实施不仅培养了学生分析问题、解决问题的能力,还适应了社会发展对复合型人才的需求,也提高了整个课程的教学效果和教学质量。     

网吧火灾自动报警系统的上位机软件设计

介绍了一个采用VisualBasic6．0编写的、在网吧中实现自动报警系统的上位机软件。系统经过了测试和实际运行，工作稳定可靠，可以实现自动报警功能，具有较高的应用和推广价值。     

禽蛋基本特性参数分析与试验

为了探讨与禽蛋大小头自动定向排列机理相关的基本特征参数分布和估算模型,测定了禽蛋的几何尺寸、质心位置、体积和质量,研究了禽蛋几何尺寸与体积、质量的关系。结果表明,禽蛋的几何尺寸和质量都呈正态分布;禽蛋质心位于禽蛋长径中点处,相对于长径与短径交点向小头侧偏移3～5 mm,质心位置称重法优于支撑法;用标准椭球体体积计算方法估算的3种禽蛋体积与实测体积相关性均在0.94以上;禽蛋质量与禽蛋估算体积的相关性优于与长径、短径、蛋形指数、禽蛋长径处椭圆面积的三元和一元线性回归,3种禽蛋质量模型的相关系数均在0.95以上。