表达MDVgB基因重组痘苗病毒的构建及保护性研究

以脂质体转染技术构建了表达鸡马立克氏病毒(MDV)gB基因的重组痘苗病毒(RVV-gB), 表明该病毒在CV-1(猴肾细胞)细胞系内能稳定传代,经腹腔1×106 PFU·羽 -1 将重组病毒、HVT冻干苗及痘苗病毒分别按试验程序,免疫1日龄SPF鸡,并于15日龄以3×10 2PFU·羽-1MDV强毒株GA株攻毒,重组病毒和HVT冻干苗匀获得较高的保护, 结果证明了gB是MDV的宿主保护性抗原.此研究为CTL应答检测奠定了基础.     

猪转移因子影响犬外周血中性粒细胞杀菌活性的最佳浓度选择

在机体的天然免疫系统中,中性粒细胞为主要的效应细胞,它是机体抵御感染性病原体的第一道防线,可以迅速穿越机体生理屏障到达局部组织.中性粒细胞的主要功能为吞噬、清除异物,杀菌和细胞毒作用,因此,中性粒细胞在抗真菌、细菌、病毒感染中的地位非常重要.     

小反刍兽疫弱毒活疫苗不同细胞毒制备的方法比较

小反刍兽疫病毒(PPRV)与牛瘟病毒(RPV)、犬瘟热病毒(CDV)、海豹瘟病毒(PDV)、海豚瘟病毒(DDV)、牛麻疹病毒(MV-K1)和人麻疹病毒(MV)等同属于副粘病毒科(Paramyxoviriade)的麻疹病毒属     

BVD—MDV细胞毒的快速粗提

采用聚乙二醇（ＰＥＧ２００００）沉淀法提取了２批牛病毒性腹泻－粘膜病病毒（ＢＶＤ－ＭＤＶ）细胞毒，经毒价及ＯＤ值测定，浓缩病毒较之浓缩毒力明显增强，病毒含量及纯度均有较大提高，将其作为检测抗原及免疫抗原，应用以ＢＶＤ－ＭＤＶ单克隆抗体的研制中，效果均较理想，试验证明，该方法具有简便，省时，费用低，实用等优点，适合基层推广应用。     

猪流行性腹泻病毒适应Vero细胞培养及以传代细胞毒制备氢氧化铝灭…

采用在病毒培养液中加５－１０μｇ／ｍｌ胰酶的培养方法，将猪流行性腹泻毒ＣＶ７７７适应于Ｖｅｒｏ细胞，并传４５代，细胞病毒规律，经免疫荧光检查阳性，电镜观察可见典型冠状病毒粒子，猪传染性胃肠炎免疫荧光检查阴性，猪流行性腹泻血清可抑制细胞病变。ＰＥＤＶ ＣＶ７７７毒株１１，２５，２８，４０及４４例传代毒的毒价分别为１０＾３．５，１０＾５．５，１０＾７．０和１０＾７．０ＴＣ１Ｄ５０／０．３ｍｌ。以１１，     

长春花4种提取物肝癌细胞毒活性的研究

用溶剂萃取法对长春花地上部分的95%乙醇提取物进行分段处理,以肝癌细胞系SMMC-7721为筛选模型,利用MTT法测定各提取物的体外肿瘤细胞毒活性,对长春花的肝癌细胞毒活性部位进行了筛选。结果表明:长春花乙醇总提取物和正丁醇提取物对体外肝癌细胞表现出较强的抑制活性,石油醚提取物﹑乙酸乙酯提取物和水提取物则没有活性。说明正丁醇提取物部分是长春花体外肝癌细胞毒活性部位。     

Cembranoid, Steroid, and Sesquiterpenoid Derivatives from the South China Sea Soft Coral Sinularia numerosa

Two new cembranoids, namely, sinumerolides A and B （1 and 2）, one new steroid, 7fl-hydroxygorgosterol （7）, along with four known cembranoid derivatives （3-6）, four known steroids （8-11）, and four known...     

Insecticidal Constructure and Bioactivities of Compounds from Ficus sarmentosa var. henryi

Insecticidal activities of the petroleum ether-, chloroform-, ethyl acetate-, and water-soluble fractions of the methanolic extract of Ficus sarmentosa var. henryi were assayed against Musca domestica adults. The chloroform- and ethyl acetate-soluble fractions were the most active with 92.6 and 88.9% mortalities (24 h after treatment) respectively. Therefore, the two fractions were combined and four compounds, isolated from the fractions by activity-guided fractionation, were elucidated as 7-hydroxycoumarin, apigenin, eriodictyol, and quercetin by spectroscopic method and displayed excellent insecticidal activities against adults of M. domestica and 4th instar larva of Aedes albopictus. Among those, 7-hydroxycoumarin showed the strongest insecticidal activities with lethal concentrations (LC₅₀) values of 72.13 μg g⁻¹ sugar and 4.87 μg mL⁻¹ (48 h after treatment) against the test insects respectively. The cytoxicities of these compounds on BTI-Tn-5Bl-4 cell were also investigated for the insecticidal mechanism and found that quercetin represented superior inhibitory activity with MTT assay and reactive oxygen species (ROS) against BTI-Tn-5Bl-4 cell, but slightly weaker than that of the positive control (azadirachtin) and significantly greater than the negative control (DMSO only). Meanwhile, eriodictyol demonstrated the strongest effect on the mitochondrial membrane potentials (MMP). In conclusion, based on their comparative toxicities to commercial insecticides and their cytotoxic effects, some of the compounds from the F. sarmentosa have potential as botanical insecticides.     

海南产木薯茎和叶挥发油的化学成分及其生物活性(英文)

为研究木薯茎和叶的挥发油成分,采用气相色谱-质谱(GC-MS)联用方法对其化学成分进行分析,鉴定了茎的挥发油中25个成分和叶挥发油中22个成分,分别占总挥发油含量的83.53%和83.46%。其中茎挥发油主要成分为棕榈酸(31.39%)、油酸(8.94%)、亚油酸(5.57%),叶挥发油主要成分为棕榈酸(16.85%)、植醇(15.02%)、异植醇(11.21%)。用滤纸片琼脂扩散法测定茎和叶挥发油的抗金黄色葡萄球菌活性和抗耐甲氧西林金黄色葡萄球菌(MASA)活性,结果表明:茎的挥发油具有抗金黄色葡萄球菌活性;叶的挥发油具有抗MASA活性。细胞毒活性结果表明,茎的挥发油对人慢性髓原白血病细胞K562有显著细胞毒活性,其IC50值为7.0μg/mL。