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排序方式: 共有265条查询结果,搜索用时 15 毫秒
1.
Influence of death struggle on the structural changes in chub mackerel muscle during chilled storage
Masashi Ando Masao Joka Satoshi Mochizuki Koh-Ichi Satoh Yasuyuki Tsukamasa Yasuo Makinodan 《Fisheries Science》2001,67(4):744-751
ABSTRACT: Chub mackerel (34–35 cm, approximately 500 g), which were caught by fishing with a rod and line at the Bungo Channel, Oita prefecture, were rested overnight in a fish preserve and either killed by decapitation (control group) or allowed to struggle in air for 30 min (struggled group). Muscle samples were excised every 4 h, and measurements on breaking strength and histological observations were done for both groups. The breaking strength of muscle in the control group was significantly higher than that in the struggled group, whereby a decrease in breaking strength was delayed for 12 h compared to the struggled group. Light microscopy showed space extension among muscle cells in association with a decrease in breaking strength. Especially in the struggled group, the extended area was larger and the difference in area was significant at the time when breaking strength showed a significant difference. Using electron microscopy, the extended area showed cut and/or disappeared collagen fibrils. From these results, it was demonstrated that struggling to death promoted the degradation of collagen fibrils and the weakening of connective tissue and, resultantly, led to the faster softening of muscle of chub mackerel. 相似文献
2.
Hitomi NAKABAYASHI Yasuyuki YAMAJI Satoshi KAGIWADA Masashi UGAKI Shigetou NAMBA 《Journal of General Plant Pathology》2002,68(2):173-176
The complete nucleotide sequence was determined for genomic RNA of White clover mosaic virus (WClMV-RC) isolated from red clover (Trifolium pratense) in Japan, It is 5843 nucleotides in length, excluding the poly(A) tail at the 3' terminus. Similar to other potexviruses,
it contains five open reading frames (ORFs 1 through 5), which putatively encode an RNA-dependent RNA polymerase (RdRp) (147
kDa), a triple gene block (TGB) (26 kDa/13 kDa/7 kDa), and a coat protein (CP) (22 kDa), respectively. The deduced amino acid
sequence of the WClMV-RC CP was identical to that of WClMV-O, one of two New Zealand isolates, but only 85% identical to that
of WClMV-M, the other New Zealand isolate, because of heterogeneity in the C-termini of CP amino acid sequences. The implication
of this CP heterogeneity is discussed.
Received 30 August 2001/ Accepted in revised form 11 January 2002 相似文献
3.
Chiho KANEKO Yasuyuki KANEKO Putu Eka SUDARYATMA Hirohisa MEKATA Yumi KIRINO Ryoji YAMAGUCHI Tamaki OKABAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):680
We isolated two pseudorabies virus (PRV) isolates (designated OT-1 and OT-2) from two hunting dogs exhibiting neurological manifestations after eating the flesh of wild boar hunted in Oita prefecture, Kyushu Island, Japan. The isolates corresponded to a previously reported PRV (MY-1 strain) isolated from a hunting dog in neighboring Miyazaki prefecture, and it clustered into genotype II based on the glycoprotein C sequence. Our results suggest that this common PRV strain may have been maintained in wild boars on Kyushu Island even though domestic pigs in this area have attained an Aujeszky’s disease-free status. 相似文献
4.
The aims of this study were to develop a new real-time quantitative PCR (QPCR) assay based on IS900 for detection and quantification of Mycobacterium avium subsp. paratuberculosis (MAP) DNA in faeces, and to use this to detect infected sheep. Both the C and S strains of MAP were detected by the QPCR assay, and no cross reactions were detected with 51 other species of mycobacteria including 10 which contained IS900-like sequences. One copy of IS900 fragment cloned into plasmid pCR2.1 and 1 fg of MAP genomic DNA were consistently detected, while in spiked faecal samples the detection limit was 10 viable MAP per gram of ovine faeces. A total of 506 individual ovine faecal samples and 27 pooled ovine faecal samples with known culture results were tested. The QPCR assay detected 68 of 69 BACTEC culture positive individual faeces and there was a strong relation between time to detection in culture and DNA quantity measured by QPCR (r= -0.70). In pooled faecal samples, QPCR also agreed with culture (kappa=0.59). MAP DNA was detected from some culture negative faecal samples from sheep exposed to MAP, suggesting that the QPCR has very high analytical sensitivity for MAP in faecal samples and detects non-viable MAP in ovine faeces. None of the faecal samples from 176 sheep that were not exposed to MAP were positive in QPCR. This is the first report of a direct faecal QPCR assay that has similar sensitivity to a gold standard radiometric culture assay. 相似文献
5.
Kobayashi T Momoi Y Iwasaki T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(9):887-892
The effects of the calcineurin inhibitors cyclosporine A (CsA) and FK506 on the mRNA expressions of various cytokines were evaluated in dogs to determine whether the effects of CsA and FK506 in dogs were similar to those in humans. The mRNA expression levels of the cytokines IL-2, IL-4, IFN-gamma and TNF-alpha were measured in PHA-stimulated canine PBMC using real-time RT-PCR after incubation with CsA or FK506 for 5 hr. Both reagents inhibited IL-2, IL-4 and IFN-gamma mRNA expressions in a dose-dependent manner. However, CsA hardly inhibited the mRNA expression of TNF-alpha. These findings are important for assessing the indications of CsA treatment in dogs. 相似文献
6.
Yoshi -Nori Nakamura Masashi Ando Manabu Seoka Ken -Ichi Kawasaki Yasuyuki Tsukamasa 《Fisheries Science》2006,72(6):1140-1146
ABSTRACT: Using full-cycle cultured (FC) Pacific bluefin tuna (body length [BL], 42.6–66.4 cm; body weight [BW], 1.66–7.40 kg, n = 15), the changes in chemical compositions and histological structure of the cephalal parts of the dorsal ordinary muscles (DOM) occurring with growth were investigated. A positive correlation ( r = 0.9644, P < 0.05) was observed between BL and BW with growth. The protein, lipid and ash contents of DOM and condition factor did not change with growth. However, the glycogen content of DOM increased ( P < 0.05) from approximately 55 cm (BL) in this study. Using optical microscopic photographs, the various shapes of muscle fibers were observed and it was noted that the muscle fiber diameter increased ( P < 0.05) with growth. Using transmission electron microscopic observation, many glycogen granules were observed in muscle fibers (especially, side of connective tissue) of DOM throughout the growth stage in this study. These results indicate that the glycogen content of DOM of FC Pacific bluefin tuna increases before the lipid with growth. 相似文献
7.
Loida O. Casalme Keisuke Katayama Yoshiki Hayakawa Kensuke Nakamura Arisa Yamauchi Yasuyuki Nogata Erina Yoshimura Fuyuhiko Matsuda Taiki Umezawa 《Marine drugs》2022,20(2)
Some derivatives of dolastatin 16, a depsipeptide natural product first obtained from the sea hare Dolabella auricularia, were synthesized through second-generation synthesis of two unusual amino acids, dolaphenvaline and dolamethylleuine. The second-generation synthesis enabled derivatizations such as functionalization of the aromatic ring in dolaphenvaline. The derivatives of fragments and whole structures were evaluated for antifouling activity against the cypris larvae of Amphibalanus amphitrite. Small fragments inhibited the settlement of the cypris larvae at potent to moderate concentrations (EC50 = 0.60-4.62 μg/mL), although dolastatin 16 with a substituent on the aromatic ring (24) was much less potent than dolastatin 16. 相似文献
8.
Seiji TSUGE Ayako FURUTANI Rie FUKUNAKA Yasuyuki KUBO Osamu HORINO 《Journal of General Plant Pathology》2001,67(1):51-57
Xanthomonas oryzae pv. oryzae (X. o. pv. oryzae) T7174 is virulent on rice cultivar IR24 and avirulent on IR-BB2. From recent reports, some virulence and avirulence factors
of plant pathogenic bacteria are transferred to plant cells through the hrp-dependent type III secretion system. In this study, we investigated the involvement of hrp genes in the compatible and the incompatible interactions between rice and X. o. pv. oryzae after co-inoculation with hrpXo mutants derived from T7174 and virulent strains. Growth of the mutants, named 74ΔHrpXo and 76ΔHrpXo, was repressed in IR24 when the mutants were applied alone. However, growth of the mutants was complemented by co-inoculation
with virulent strains. Growth of bioluminescent hrpXo mutant 76ΔHrpXo in IR24 and its growth in IR-BB2 after co-inoculation with T7133, which is virulent on both cultivars, was equally complemented,
as detected by bioluminescence from the mutant. On the other hand, only partial complementation of growth of T7174L76, which
is a bioluminescent and pathogenic derivative of T7174, by T7133 was observed in IR-BB2. Thus, growth of the hrpXo mutant of X. o. pv. oryzae was complemented by virulent strains in both susceptible and resistant rice leaves with the parental strain.
Received 21 July 2000/ Accepted in revised form 26 October 2000 相似文献
9.
10.
This study examined the residence pattern of ayu larvae and juveniles occurring in the surf zone of an extensive sandy beach,
in the north part of the Sea of Japan. In this region, ayu larvae mainly hatched in October and November, generally appeared
in the surf zone in hatching order, and were continuously present until the following January. During a short period after
mid- to late January when the water temperature fell below 10°C, ayu larvae and juveniles concurrently migrated from the surf
zone regardless of their size and age, resulting in a longer term residence for earlier-hatched ayu. This was in contrast
to studies in other waters, which found that early-hatched ayu have a short-term residence and late-hatched a long-term residence.
Furthermore, faster growth that enables ayu to reach the maximum residence size (around 40 mm BL) in the surf zone during
the period when the water temperature was still over 10°C might lead to earlier migration and a shorter residence term. This
process might be applicable only to the early-hatched group (defined as individuals hatched in late September and October)
and depends on the size of each individual, thus leading to a gradual migration of larger individuals as they attain maximum
residence size. 相似文献