Conjunctival habronemiasis in a square‐lipped rhinoceros (Ceratotherium simum)

A captive female square‐lipped rhinoceros born in 1993 had been showing intermittent signs of bilateral conjunctivitis and conjunctival proliferation since 1998. Periodic improvement was noted, especially in winter, but overall the condition had deteriorated over the years. Treatment with various topical, intralesional, and systemic antibiotics and glucocorticosteroids was largely ineffective, as were repeated dewormings. No primary cause for these lesions was found in biopsies taken in 2000 and 2006, although a severe infiltrate of numerous eosinophils was observed in the latter. As the condition worsened, secondary corneal changes were noted, and eventually vision was lost due to proliferative conjunctival tissue. Aggressive resection of the proliferating tissue in 2013 restored vision and submitted biopsies yielded a diagnosis of severe allergic conjunctivitis, eosinophilic granuloma, and habronematid (Habronema or Draschia) larval infection. As no other rhinoceros in the herd was affected, including two calves born to the patient who were in close contact with their mother, it was concluded the presentation was most likely due to a hypersensitivity reaction to the dead or dying larvae. Fly repellent is now regularly applied around the eye of this rhinoceros, and a protective face mask has been fitted. Ongoing periodic relapses are treated with oral ivermectin, topical antibiotics, and steroids.     

Sudden death of a bearded vulture (Gypaetus barbatus) possibly caused by Newcastle disease virus

An adult female bearded vulture (Gypaetus barbatus) in the Tel Aviv University Research Zoo was found dead without previous clinical signs. The predominant pathologic changes were considerable bloody content in the intestines and enlargement of the liver, which had a rubbery consistency with color changes. Microscopic lesions consisted of multifocal histiocytic infiltration in the liver. Newcastle disease virus (NDV) was isolated from a cloacal swab and from the lungs and liver. Intracerebral pathogenicity index of the virus, as estimated in 1-day-old chicks, was repeated three times and had an average value of 1.68, indicating a velogenic strain. Numerous Clostridium septicum bacteria were found on the intestinal surface, but bioassays in which they were orally administered into chickens and mice revealed that, even though they were heavily multiplied in the intestines, they were nonpathogenic. It seems that NDV, documented for the first time in a bearded vulture in Israel, was the likely cause of sudden death.     

Pathogenic and Nonpathogenic Lifestyles in Colletotrichum acutatum from Strawberry and Other Plants

ABSTRACT Anthracnose is one of the major fungal diseases of strawberry occurring worldwide. In Israel, the disease is caused primarily by the species Colletotrichum acutatum. The pathogen causes black spot on fruit, root necrosis, and crown rot resulting in mortality of transplants in the field. The host range and specificity of C. acutatum from strawberry was examined on pepper, eggplant, tomato, bean, and strawberry under greenhouse conditions. The fungus was recovered from all plant species over a 3-month period but caused disease symptoms only on strawberry. Epiphytic and endophytic (colonization) fungal growth in the different plant species was confirmed by reisolation from leaf tissues and by polymerase chain reaction (PCR)-specific primer amplification. C. acutatum was also isolated from healthy looking, asymptomatic plants of the weed genera Vicia and Conyza. Isolates that were recovered from the weeds caused disease symptoms on strawberry and were positively identified as C. acutatum by PCR. The habitation of a large number of plant species, including weeds, by C. acutatum suggests that, although it causes disease only on strawberry and anemone in Israel, this fungus can persist on many other plant species. Therefore, plants that are not considered hosts of C. acutatum may serve as a potential inoculum source for strawberry infection and permit survival of the pathogen between seasons.     

The newest leafminer pest in Israel,Liriomyza huidobrensis

The pea leafminer,Liriomyza huidobrensis (Blanchard), recently introduced into Israel, has proven to be extremely proliferous and difficult to control. It is a much more serious pest of vegetable and flower crops than the previously introducedLiriomyza trifolii (Burgess). Photosynthesis is reduced and cosmetic damage is incurred when adult flies stipple plant leaves with feeding punctures and larvae mine the leaves. This paper will review briefly the history, biology and potential control measures of the pea leafminer.     

Emamectin,a novel insecticide for controlling field crop pests

Emamectin is a macrocyclic lactone insecticide with low toxicity to non-target organisms and the environment, and is considered an important component in pest-management programmes for controlling field crop pests. It is a powerful compound for controlling the cotton bollworm Helicoverpa armigera (Hübner). A spray concentration of 25 mg AI litre-1 in a cotton field resulted in over 90% suppression of H armigera larvae up to day 28 after treatment, while similar mortality of the Egyptian cotton leafworm Spodoptera littoralis Boisduval, under the same conditions, was maintained for 3 days only. Emamectin is a potent compound for controlling the western flower thrips Frankliniella occidentalis (Pergande) under both laboratory and field conditions and its activity on adults was over 10-fold greater than that of abamectin. Spray concentrations of 10 and 50 mg AI litre-1 in Ageratum houstonianum Mill flowers resulted in total suppression of adults up to day 11 and of larvae up to day 20 after treatment. Under standard laboratory conditions, emamectin exhibits a considerable activity on the whitefly Bemisia tabaci (Gennadius) and the leafminer Liriomyza huidobrensis (Blanchard). Further studies are required to evaluate its potential activity on the latter pests under field conditions.     

Tonometry in three herbivorous wildlife species

Tonometry was performed to estimate intraocular pressure (IOP) in 12 Nubian ibexes ( Capra ibex nubiana ), 10 Grant zebras ( Equus burchelli  ) and five Arabian oryxes ( Oryx leucoryx ), using both applanation (Tono-Pen) and/or indentation (Schiotz) tonometers. Animals were anesthetized with a mixture of etorphine hydrochloride and acepromazine maleate. Mean (± SD) IOP in the ibex was 17.95 ± 4.78 mmHg (24 eyes, indentation tonometry). In the zebra, indentation tonometry (20 eyes) yielded a mean IOP of 25.30 ± 3.06 mmHg, and applanation tonometry (six eyes) yielded a mean IOP of 29.47 ± 3.43 mmHg. In the oryx, indentation tonometry (five eyes) yielded a mean IOP of 22.68 ± 8.15 mmHg, and applanation tonometry (10 eyes) yielded a mean IOP of 11.76 ± 3.43 mmHg. There were no significant effects of gender, age, weight, side or reading number on the IOP measured in any of the three species. No significant differences were found between the IOP of the three species, nor between the readings of the two instruments, although some of the P -values were close to the significance level.     

The viking biological investigation: preliminary results

Three different types of biological experiments on samples of martian surface material ("soil") were conducted inside the Viking lander. In the carbon assimilation or pyrolytic release experiment, (14)CO(2) and (14)CO were exposed to soil in the presence of light. A small amount of gas was found to be converted into organic material. Heat treatment of a duplicate sample prevented such conversion. In the gas exchange experiment, soil was first humidified (exposed to water vapor) for 6 sols and then wet with a complex aqueous solution of metabolites. The gas above the soil was monitored by gas chromatography. A substantial amount of O(2) was detected in the first chromatogram taken 2.8 hours after humidification. Subsequent analyses revealed that significant increases in CO(2) and only small changes in N(2) had also occurred. In the labeled release experiment, soil was moistened with a solution containing several (14)C-labeled organic compounds. A substantial evolution of radioactive gas was registered but did not occur with a duplicate heat-treated sample. Alternative chemical and biological interpretations are possible for these preliminary data. The experiments are still in process, and these results so far do not allow a decision regarding the existence of life on the plonet Mars.     

Planetary contamination I: the problem and the agreements

The sterility requirements for landed spacecraft tentatively adopted in the COSPAR resolution of 1964 are so severe as to pose a major obstacle to planetary exploration. This by itself would not justify modification of the re quirements, since preservation of the biological integrity of Mars is essential for proper exploration of the planet. However, when the physical and biological assumptions underlying the COSPAR recommendations are com pared with actual conditions on Mars, as established by recent observations, it becomes apparent that the COSPAR as sumptions are unrealistic in important respects. Specifically, the belief that eolian erosion on Mars can effect the release of spores trapped in the interior of solids in periods of time that are short compared with the time scale of the unmanned space program is unsup ported by either observation or theory. On the contrary, the analysis suggests that rates of eolian erosion on Mars are very low. Similarly, present knowledge of the Martian environment opposes the view that terrestrial microorganisms would readily contaminate the planet. The combination of dryness, lack of oxygen, and high ultraviolet flux makes the surface of Mars peculiarly unsuit able for the multiplication of terrestrial organisms. Recent studies give little sup port to the proposal that significant areas of geothermal activity exist on Mars. These various findings suggest that the COSPAR-recommended constraints could be substantially relaxed without compromising to any significant degree the biological condition of Mars. In particular, a distinction needs to be made between microorganisms trapped in solids and those on exposed sur faces of landed spacecraft. Surface sterility is an unconditional require ment, in the sense that it is imposed by considerations unrelated to the nature of the Martian environment. Sterilization of the interior of solids to the extreme level recommended by COSPAR, however, is based on the as sumption that entrapped organisms con stitute a substantial hazard to the ecology of Mars. This assumption now seems unjustified, and the need for a high degree of interior sterility is doubt ful. Current spacecraft-sterilization pol icies should be revised accordingly.     

Submicrometer Superconducting YBa2Cu3O6+x Particles Made by a Low-Temperature Synthetic Route

Evidence suggests that superconducting, orthorhombic YBa(2)Cu(3)O(6+x)+ (x greater, similar 0.5) is always produced by oxidation of the oxygen-deficient, tetragonal form (x less, similar 0.5) of this phase (commonly referred to as 123). A synthetic route whereby solution-derived, carbon-free precursors are decomposed at 650 degrees to 700 degrees C in inert atmosphere to yield tetragonal 123 is now available. Appropriate precursors include hydrated oxides derived from the hydrolysis of organometallic solutions and aqueous solution-derived hyponitrites. Subsequent oxidation of the tetragonal phase at 400 degrees C results in submicrometer particles of orthorhombic 123. Superconductivity (T(c) onset approximately 87 K) has been confirmed in these materials by both Meissner effect and specific-heat measurements.