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MITSUHIRO MATSUO HIDEKI MICHINAGA HIROYUKI TERAO EIJI TSUZUKI 《Weed Biology and Management》2004,4(3):148-153
Seed germination, seedling emergence, and the morphological characteristics of juvenile seedlings of Commelina benghalensis L. were observed. For aerial seeds collected in September and October, seedling emergence peaked in April and June for large seeds and from June to August for small seeds, whereas seedling emergence for large seeds collected in November showed peaks in March and April under natural rainfall conditions, and in April and June under irrigation conditions. Seedlings from small seeds emerged intermittently over a longer period from April to August under both conditions. Aerial seeds of C. benghalensis germinated on wet filter paper on the second day after seeding (DAS) for large seeds and the fourth DAS for small seeds. The germination percentage for large seeds was higher than that for small seeds by the 14th DAS. The germination percentage for large aerial seeds showed no significant difference between light and dark conditions. However, the percentage for small aerial seeds was higher under light than under dark conditions. Seedlings from large aerial seeds emerged on the third DAS at 0–50 mm soil depths. The percentage of emergence at 0 and 1 mm soil depths increased until the 30th DAS, whereas those at soil depths of 5–50 mm showed no change after the 9th DAS. There was no emergence at a soil depth of 100 mm. Seedlings from small aerial seeds emerged on the 6th DAS at 0–1 mm soil depths, with the percentage increasing until the 30th DAS. Although seedlings at 5 and 10 mm soil depths also emerged on the 6th DAS, there was no change in the percentage after the 12th DAS. There was no emergence at soil depths of 20–100 mm. The hypocotyl and taenia (part of the cotyledon connected to the seed) in juvenile seedlings that emerged from soil depths of 50 mm were longer than those in seedlings emerging from a soil depth of 1 mm. 相似文献
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Allele frequencies of the extension locus encoding the melanocortin-1 receptor in Japanese and Korean cattle 总被引:2,自引:0,他引:2
Shinji SASAZAKI Munehiro USUI Hideyuki MANNEN Chihiro HIURA Soichi TSUJI 《Animal Science Journal》2005,76(2):129-132
In order to estimate the influence of the Extension (E) locus in cattle coat color, the melanocortin‐1 receptor (MC1R) gene in Japanese Black, Japanese Brown and Korean (Hanwoo) cattle were sequenced. The sequences of the coding region revealed three alleles (ED, E+ and e), which were previously reported. Polymerase chain reaction‐restriction fragment length polymorphism was performed to investigate the gene frequencies of the three breeds. Japanese Black was almost composed of ED and E+ individuals, ED = 0.481 and E+ = 0.514, and no homozygous e/e, therefore that is consistent with the hypothesis that ED and E+ induce black pigment synthesis. Allele frequencies between Japanese Brown and Hanwoo were obviously different; however, recessive red e allele frequency was 0.038 for Japanese Brown and 0.948 for Hanwoo, even though both breeds have quite similar coat colors (ranging from yellowish brown to dark brown including a red coat color). This result suggested that other genes are also associated with a coat color of red and brown in cattle. 相似文献
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Shuhei ENJOJI Ryotaro YABE Nobuyuki FUJIWARA Shunya TSUJI Michael P. VITEK Takuya MIZUNO Takayuki NAKAGAWA Tatsuya USUI Takashi OHAMA Koichi SATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1451-1456
Canine melanoma is one of the most important diseases in small animal medicine.
Protein phosphatase 2A (PP2A), a well conserved serine/threonine phosphatase, plays a
critical role as a tumor suppressor. SET/I2PP2A is an endogenous inhibitor for PP2A, which
directly binds to PP2A and suppresses its phosphatase activity. Elevated SET protein
levels have been reported to exacerbate human tumor progression. The role of SET in canine
melanoma, however, has not been understood. Here, we investigated the potential
therapeutic role for SET inhibitors in canine melanoma. The expression of SET protein was
observed in 6 canine melanoma cell lines. We used CMeC-1 cells (primary origin) and CMeC-2
cells (metastatic origin) to generate cell lines stably expressing SET-targeting shRNAs.
Knockdown of SET expression in CMeC-2, but not in CMeC-1, leads to decreased cell
proliferation, invasion and colony formation. Phosphorylation level of p70 S6 kinase was
decreased by SET knockdown in CMeC-2, suggesting the involvement of mTOR (mammalian target
of rapamycin)/p70 S6 kinase signaling. The SET inhibitors, OP449 and FTY720, more
effectively killed CMeC-2 than CMeC-1. We observed PP2A activation in CMeC-2 treated with
OP449 and FTY720. These results demonstrated the potential therapeutic application of SET
inhibitors for canine melanoma. 相似文献
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