全文获取类型
收费全文 | 348篇 |
免费 | 15篇 |
国内免费 | 3篇 |
专业分类
林业 | 35篇 |
农学 | 12篇 |
28篇 | |
综合类 | 14篇 |
农作物 | 9篇 |
水产渔业 | 14篇 |
畜牧兽医 | 232篇 |
植物保护 | 22篇 |
出版年
2022年 | 4篇 |
2021年 | 6篇 |
2020年 | 5篇 |
2019年 | 3篇 |
2018年 | 5篇 |
2017年 | 6篇 |
2016年 | 13篇 |
2015年 | 9篇 |
2014年 | 14篇 |
2013年 | 18篇 |
2012年 | 17篇 |
2011年 | 15篇 |
2010年 | 12篇 |
2009年 | 9篇 |
2008年 | 18篇 |
2007年 | 13篇 |
2006年 | 20篇 |
2005年 | 18篇 |
2004年 | 22篇 |
2003年 | 18篇 |
2002年 | 14篇 |
2001年 | 8篇 |
2000年 | 8篇 |
1999年 | 10篇 |
1998年 | 3篇 |
1997年 | 3篇 |
1995年 | 4篇 |
1994年 | 3篇 |
1992年 | 3篇 |
1991年 | 7篇 |
1990年 | 1篇 |
1989年 | 6篇 |
1988年 | 10篇 |
1987年 | 4篇 |
1986年 | 7篇 |
1985年 | 6篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1980年 | 2篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1975年 | 3篇 |
1974年 | 2篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1970年 | 1篇 |
1969年 | 4篇 |
1968年 | 2篇 |
1967年 | 1篇 |
1964年 | 1篇 |
排序方式: 共有366条查询结果,搜索用时 15 毫秒
1.
M Onuma Y Yasutomi M Yamamoto S Watarai T Yasuda Y Kawakami 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1989,36(2):139-147
Monoclonal antibody c143 against tumor-associated antigen (TAA) expressed on bovine leukemia cells was conjugated to liposomes containing adriamycin (ADM), and therapeutic effects of conjugates were examined in leukemic or preleukemic cows to prevent the progression of the disease. Five cows with TAA-positive in their peripheral blood lymphocytes were divided into two groups. Each group of cows received 4 injections of ADM alone (0.4 mg/kg) or c143-conjugated liposomes containing the same dose of ADM (L-AMD-c143) through the jugular vein at about 4 day intervals. In three animals treated with L-ADM-c143, the TAA-positive cells gradually decreased with treatment and finally two animals became TAA-negative during a 6 week period and a 14 week period after treatment, respectively. In the control, two animals treated with ADM alone showed only a slight decrease of TAA-positive cells. 相似文献
2.
3.
Okayama T Matsuno Y Yasuda N Tsukui T Suzuta Y Koyanagi M Sakaguchi M Ishii Y Olivry T Masuda K 《Veterinary immunology and immunopathology》2011,139(2-4):99-106
As IgE plays a pivotal role in type I hypersensitivity-mediated allergic diseases, it is valuable to measure absolute quantity of serum antigen-specific IgE for clinical and research purposes. Here we describe a novel ELISA system that enables quantification of antigen-specific IgE in ng/ml in dogs. A newly developed monoclonal antibody (CRE-DM) was shown to recognize canine and mouse IgE equally in a dose dependent manner, but it did not recognize canine IgG. The reactivity of CRE-DM to canine IgE was also confirmed by an inhibition ELISA using canine IgE as an inhibitor and the maximum inhibition rate was 91.3%. In order to know whether canine IgE specific to an allergen could be quantitatively measured with an ELISA using CRE-DM, we established a quantitative ELISA that could measure canine IgE recognizing Cry j 1, one of the major allergens of Japanese cedar pollen. In this ELISA, a standard curve was created by using concentration-predetermined Cry j 1-specific monoclonal mouse IgE. According to the standard curve, the concentration of Cry j 1-specific IgE in dogs that were experimentally sensitized to Japanese cedar pollen could be calculated and determined in ng/ml. The specificity of the Cry j 1-specific IgE ELISA using CRE-DM was also confirmed by inhibition ELISA using canine IgE as an inhibitor and the inhibition rate was 97.0%. Reproducibility of the ELISA in three independent assays was determined using groups of pooled canine sera whose Cry j 1-IgE titers ranged from 155.9 to 888.2 ng/ml. Intra- and inter-assay reproducibility was determined with coefficient of variation ranging between 3.1-5.2% and 2.2-8.0%, respectively. These results demonstrated that the ELISA utilizing CRE-DM was a specific, reliable and robust new laboratory test that could quantify absolute amount of antigen-specific IgE in canine serum. The ELISA will serve as a useful tool in the clinics to evaluate the change of serum IgE titers during anti-allergic treatments as well as during seasonal fluctuation of allergen exposure. 相似文献
4.
Syakalima O Choongo K Nakazato Y Onuma M Sugimoto C Tsubota T Fukushi H Yoshida M Itagaki T Yasuda J 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(3):315-318
Exposure and ecological risks to heavy metals (copper, zinc, manganese, iron) at Lochnivar and Blue Lagoon National Parks in wildlife dependent on the Kafue river contaminated with mining waste was evaluated. Samples included water, fish, grasses and Kafue Lechwe (Kobus leche kafuensis) liver. At both parks copper ranged from 0.03-0.04 mg/l; 3.0-6.0 mg/kg; 11.0-44.0 mg/kg; trace -199.0 mg/kg; while zinc was 0.01 mg/l; 32.0-82.0 mg/kg; 15.0-21.0 mg/kg; and 52.0-138.0 mg/kg; in water, fish, grasses and lechwe, respectively. Manganese ranges were 0.15-0.16 mg/l; 7.0-18.0 mg/kg; 51.0-145.0 mg/kg; and 40.0-53.0 mg/kg while iron ranges were 0.13-0.14 mg/l; 26.0-134.0 mg/kg; 1766.0-1797.0 mg/kg; and 131.0-856.0 mg/kg; in water, fish, grasses and lechwe, respectively. Levels in all samples except water were high indicating potential for adverse effects. 相似文献
5.
Namangala B Yokoyama N Ikehara Y Taguchi O Tsujimura K Sugimoto C Inoue N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(8):751-759
Despite the immense socio-economic repercussions of African trypanosomosis (AT), there is currently no effective control measure against the disease. Characterization of mechanisms governing resistance and/or susceptibility to AT could suggest interventions that might lead to more effective disease control. The present study was designed in an attempt to address the possible role of CD4+CD25+ T cells during an acute lethal infection of mice with Trypanosoma congolense, the causative agent of AT in domestic animals, through selective depletion using anti-CD25 monoclonal antibody. Accordingly, CD4+CD25+ T-cell-depletion resulted in a significant reduction or delay in parasitemia, pathology, and mortality, as compared to controls. The apparent resistance in CD4+CD25+-T-cell-depleted mice correlated with a profound suppression of Th2 cytokines in vitro and in vivo, culminating in a net Th1 cytokine environment. Cumulatively, these findings suggest that CD4+CD25+ T-cell- depletion improves the trypanotolerance of highly susceptible BALB/c mice acutely infected with the lethal T. congolense. 相似文献
6.
Hiroto Miura Takuya Hashimoto Yukiko Kawanishi Hiroki Kawauchi Ryo Inoue Noriaki Shoji Kunihiko Saito Mario Sekiya Yosuke Saito Jumpei Yasuda Chiemi Yonezawa Tetsushiro Endo Hirotaka Kasuya Yutaka Suzuki Yasuo Kobayashi Satoshi Koike 《Animal Science Journal》2021,92(1):e13601
The rumen microbiota comprises a vast range of bacterial taxa, which may affect the production of high-quality meat in Japanese Black cattle. The aim of this study was to identify core rumen microbiota in rumen fluid samples collected from 74 Japanese Black cattle raised under different dietary conditions using 16S rRNA gene amplicon sequencing. In the rumen of fattening Japanese Black cattle, 10 bacterial taxa, showing >1% average relative abundance and >95% prevalence, irrespective of the dietary conditions and the fattening periods, were identified as the core rumen bacterial taxa, which accounted for approximately 80% of the rumen microbiota in Japanese Black cattle. Additionally, population dynamics of the core rumen bacterial taxa revealed two distinct patterns: Prevotella spp. and unclassified Bacteroidales decreased in the mid-fattening period, whereas unclassified Clostridiales, unclassified Ruminococcaceae, Ruminococcus spp., and unclassified Christensenellaceae increased during the same period. Therefore, the present study reports the wide distribution of the core rumen bacterial taxa in Japanese Black cattle, and the complementary nature of the population dynamics of these core taxa, which may ensure stable rumen fermentation during the fattening period. 相似文献
7.
Comparison and phylogenetic analysis of the heat shock protein 70 gene of Babesia parasites from dogs 总被引:1,自引:0,他引:1
Yamasaki M Inokuma H Sugimoto C Shaw SE Aktas M Yabsley MJ Yamato O Maede Y 《Veterinary parasitology》2007,145(3-4):217-227
The heat shock protein 70 (hsp70) genes of Babesia gibsoni, B. canis canis, B. canis vogeli, and B. canis rossi isolated from infected dogs were cloned by polymerase chain reaction (PCR) and sequenced. In the nucleotide sequence and the predicted amino acid sequence of the gene, the parasites were very similar to each other. The nucleotide sequences of the hsp70 gene had more variety than those of 18S nuclear subunit ribosomal DNA (18S rDNA). A phylogenetic analysis of these sequences and comparisons with sequences from other Babesia and Theileria species revealed that all canine babesial isolates analyzed in the present study were closely related to each other and formed one cluster. Additionally, a phylogenetic analysis of Babesia and Theileria species showed that these parasites could be divided into three groups: group A including canine babesial isolates, B. divergens, B. odocoilei, B. bovis, B. caballi, and B. ovis; group B including Theileria annulata, T. orientalis, and T. cervi; and group C including B. microti and B. rodhaini. These results suggested that a phylogenetic analysis of the hsp70 gene sequence might be helpful in classifying Babesia and Theileria species, and that canine babesial isolates might be closely related to each other, indicating their evolution from the same ancestry. 相似文献
8.
Ohmori K Masuda K Kawarai S Yasuda N Sakaguchi M Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(8):865-867
IgE-reactive beef components were examined by an immunoblot analysis using a serum from a dog with food hypersensitivity against beef. The immunoblot analysis revealed a distinct band at approximately 66 kDa and a faint band at approximately 50 kDa. The immunoblot analysis for serum IgE reactivity to bovine serum albumin (BSA) also revealed a positive band at 66 kDa. Serum IgE reactivity to the 66-kDa protein of beef was diminished by pre-incubating the serum sample with BSA. Furthermore, a positive reaction to BSA was detected in intradermal testing in the dog. These results clearly indicated that BSA was an IgE-reactive beef component in the dog with food hypersensitivity against beef. 相似文献
9.
Yasuko Mizoguchi Yoshikazu Ohtani Satoru Takanashi Hiroki Iwata Yukio Yasuda Yuichiro Nakai 《Journal of Forest Research》2012,17(3):283-295
Carbon dioxide (CO2) flux was measured above the forest at the Fujiyoshida site on the northern slope of Mount Fuji in Japan in 2000?C2008 using an eddy covariance technique. The forest mainly consists of Japanese red pine (Pinus densiflora) and Japanese holly (Ilex pedunculosa). The 9-year average of monthly mean net ecosystem production (NEP) ranged from ?0.1?g?C?m?2?day?1 in January to 2.5?g?C?m?2?day?1 in May. The maximum net uptake was observed in May, although gross primary production (GPP) was highest in July. Variation in the leaf amount did not notably affect seasonal variation in GPP. This site was characterized by carbon uptake even in winter, if the meteorological conditions were conducive for photosynthesis and a resulting long period of carbon uptake. The 9-year averages of annual NEP, GPP, and ecosystem respiration (RE) were 388, 1,802, and 1,413?g?C?m?2?year?1, respectively. The annual NEP was lowest in 2003 and highest in 2004 over the 9?years. Year-to-year variability of NEP mainly depended on air temperature and photosynthetically active radiation in summer, and the dependence of the deviation of annual NEP on that of GPP was greater than that of RE. Long-term observational data indicated that the carbon uptake ability at the study site was at a moderate level in comparison with other temperate humid evergreen forests around the world. These data also indicated that the site had a high carbon uptake ability compared with other deciduous forests in Japan because of the duration of carbon uptake. 相似文献
10.
Chihiro Kayo Seiji Hashimoto Atsunori Numata Masanori Hamada 《Journal of Wood Science》2011,57(3):234-240
We compared the greenhouse gas (GHG) emissions from a log pile (LP) to those from a sand compaction pile (SCP) and from cement
deep mixing (CDM) as measures against soil liquefaction, assuming that forest and waste management scenarios influence the
GHG (CO2, CH4, and N2O) balance of wood. We found little difference between the LP and SCP methods with respect to GHG emissions from fossil fuel
and limestone consumption. However, GHG emissions from the CDM method were seven times higher than emissions from the LP method.
In the GHG balance of wood, when the percentage of CH4 emissions from carbon in underground wood was lower than 3.3%, permanent storage in the log achieved greater reductions in
GHG emissions than using the waste log as fuel in place of coal or heavy oil. In order to obtain reductions in GHG emissions
by replacing SCPs or CDM with LPs, sustainable forest management with reforestation and prevention of CH4 emissions from the underground log are essential. Using reforestation, permanent storage of the log, no CH4 emission from the log, and using logging residues instead of coal, the LP can achieve reductions in GHG emissions of 121
tonnes of CO2 per 100 m2 of improvement area by replacing CDM. 相似文献