全文获取类型
收费全文 | 1635篇 |
免费 | 77篇 |
国内免费 | 130篇 |
专业分类
林业 | 111篇 |
农学 | 83篇 |
基础科学 | 137篇 |
164篇 | |
综合类 | 770篇 |
农作物 | 120篇 |
水产渔业 | 101篇 |
畜牧兽医 | 173篇 |
园艺 | 109篇 |
植物保护 | 74篇 |
出版年
2024年 | 10篇 |
2023年 | 33篇 |
2022年 | 65篇 |
2021年 | 88篇 |
2020年 | 69篇 |
2019年 | 69篇 |
2018年 | 43篇 |
2017年 | 48篇 |
2016年 | 42篇 |
2015年 | 80篇 |
2014年 | 91篇 |
2013年 | 75篇 |
2012年 | 118篇 |
2011年 | 147篇 |
2010年 | 138篇 |
2009年 | 114篇 |
2008年 | 137篇 |
2007年 | 129篇 |
2006年 | 112篇 |
2005年 | 61篇 |
2004年 | 41篇 |
2003年 | 27篇 |
2002年 | 29篇 |
2001年 | 32篇 |
2000年 | 25篇 |
1999年 | 12篇 |
1987年 | 1篇 |
1963年 | 1篇 |
1962年 | 1篇 |
1961年 | 2篇 |
1959年 | 1篇 |
1955年 | 1篇 |
排序方式: 共有1842条查询结果,搜索用时 15 毫秒
1.
2.
用通径分析的方法分析了不同形态硫对土壤有机质的作用。结果表明 :在影响土壤有机质的诸多硫组分中 ,以全硫为主 ,其次是未知态硫和全硫的共同作用 ,再次是碳键硫和全硫的共同作用 ,脂键硫排在第九位。有机硫起主要作用 相似文献
3.
LIN(Local InterconnectNetwork)协议是面向车辆低端分布式应用的一类串行通信协议。LIN设计目标作为CAN(ControllerArea Network)的下层网络,同CAN相结合可构成车辆应用中的分层网络结构。本文对LIN总线进行了介绍,设计了CAN-LIN总线网络拓扑结构图,并对CAN-LIN节点的软硬件进行了设计调试,最后连接车身电器试验台进行了测试。 相似文献
4.
AIM:To examine whether calcitonin gene-related peptide (CGRP) enhances nitric oxide (NO) level in pulmonary circulation blood and observe the influence of CGRP on mean pulmonary artery pressure (mPAP) in rabbits with acute lung injury (ALI) caused by oleic acid.METHODS: The level of NO was assessed by measuring the presence of nitrite in cervical artery blood by the Griess reaction, mPAP was measured with right ventricular catheter.RESULTS:The level of nitrite in cervical artery blood was significantly increased and the mPAP was markedly reduced after administration of CGRP intravenousely.CONCLUSION:CGRP enhanced the NO level of pulmonary circulation blood and reduces the mPAP significantly in rabbits with ALI. 相似文献
5.
6.
7.
8.
AIM: To observe the antiproliferative effect of c-myc antisense oligonucleotide in rat thymus lymphocytes. METHODS: Rat thymus lymphocytes were separated by Ficoll-Urografin density gradient centrifugation. Lipofectin was used to introduce antisense, sense and mismatched oligonucleotides for c-myc to rat thymus lymphocytes. The antiproliferative effect was assayed by incorporation of [3H]-TdR and MTS cell proliferation assay. TR-PCR was used to detect the expression of c-myc mRNA. RESULTS: c-myc antisense oligonucleotide inhibited ratthymus lymphocytes proliferation[(0.14±0.03)A vs(0.32±0.16)A,P<0.05],but this ef ect had no relationship with the concentration of c-myc antisense oligonucleot ide.c-myc antisense oligonucleotide decreased the expression of c-myc mRNA in rat thymus lymphocytes. CONCLUSION: c-myc antisense oligonucleotide inhibited rat thymus lymphocyte proliferation. 相似文献
9.
AIM: To investigate effect of atrial natriuretic peptide (ANP) on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in rat. METHODS: Mean arterial blood pressure (MAP) was recorded with model 6280 physiology intelligentialize grapher, nitric oxide (NO) and endothelin (ET) concentrations in plasma were measured after lipopolysaccharide (LPS) or following LPS ,ANP was injected into vein in rats. After experiment,lung water as well as pulmonary histopathological changes was measured and observed, respectively. RESULTS: Administration of LPS elicited a persistence decrease in MAP (8.1 kPa±2.6 kPa,at 4 h,P<0.01 vs control); NO and ET concentration in plasma was evident higher than that in control group, respectively (P<0.01); Wet-dry ratio of lung was higher than that in control group (5.15±0.43,at 4 h) (P<0.05); Alveolus detelectasis was observed and pulmonary mesenchyme was thicker than that in control group. No erythrocyte and leukocytes in alveolus,which show an interstitial pulmonary edema, was observed in LPS+ANP group, ANP maintained MAP at higher levels (13.35 kPa±2.93 kPa, at 4 h, P<0.05 vs LPS) after an transient decline when LPS was injected; NO and ET concentration of plasma had all significantly decrease, respectively (P<0.05 vs LPS, at 4 h); Wet-dry ratio of lung was lower than LPS group (4.57±0.35, P<0.05). Compared with control group the ratio was not evident difference (P>0.05); The histopathological of lung displayed markedly improved. CONCLUSION: ANP attenuates ALI induced by LPS in the rat. The effect of ANP may be via decreasing secretion of ET,NO and regulation arterial blood pressure. 相似文献
10.
PP2C是一大类重要的蛋白磷酸酶,广泛参与不同的逆境胁迫响应。为了解PP2C基因在干旱响应中的功能,以亚洲棉石系亚1号为材料,利用RT-PCR方法从中克隆了GaPP2C24基因,并对该基因进行了生物信息学分析,荧光定量PCR研究了其在亚洲棉不同组织和干旱胁迫下的表达情况。结果表明,GaPP2C24基因CDS序列全长为1 251 bp,编码416个氨基酸。序列同源比对发现,GaPP2C24与其他植物的PP2C氨基酸序列有较高的相似性,其中与可可树(EOY33930.1)、黄麻(OMO91132.1)的相似性分别为85%,84%。进化分析表明,GaPP2C24与同属锦葵目的黄麻聚在一支,亲缘关系最近,与可可树的亲缘关系次之。实时荧光定量PCR结果表明,GaPP2C24基因在子叶、下胚轴、胚根、叶、茎和根均有表达,且在茎中的表达量最高,根中次之。GaPP2C24受干旱胁迫的诱导表达,其在根和叶片中的相对表达量在处理1 h后最高,分别达到对照的53.9,6.9倍,推测该基因在棉花干旱响应中有重要作用。 相似文献