应用间接荧光抗体技术快速检测花鲈病原菌——鳗弧菌

以花鲈（Lateolabrax japonicus)弧菌病的病原菌-鳗弧菌（Vibrio anguillarum)W-1为抗原，制备兔抗血清；利用异硫氰酸荧光素标记的羊抗兔免疫球蛋白（FITC-IgG）为荧光标记二抗，并以罗丹明标记的牛血清白蛋白为背景染色，建立检测鳗弧菌的间接荧光抗体快速检测技术。应用该技术对人工感染后的花鲈组织（肌肉、鳃、肠、肾）样品和养殖水体样品进行了鳗弧菌检测，结果显示间接荧光抗体技术不仅可以用于诊断发病的感染花鲈，也可用于检测带菌状态或未发病的感染花鲈。     

The effects of nitrogen sludge load and DO on high concentration nitrous nitrifying system

The purpose of this paper is to investigate the impact of nitrogen sludge load and dissolved oxygen(DO) on nitrite nitrification system. The following conclusions have been drawn based on the experimental data and analysis results: 1) The ammonium degradation rate and the nitrous accumulation rate of high concentration nitrous nitrifying system decline with the increase of NH+4-N sludge load. When HRT is less than two days, the ammonium degradation rate rapidly declines to 25%~29% with the increase of NH+4-N sludge load. When HRT is beyond two days, the ammonium rate of the system slowly falls to 50%~60% with the NH+4-N sludge load increased. When HRT is 2.5 days or 3 days, the declined trend of the system nitrous accumulation rate is not obvious with the NH+4-N sludge load increased. When HRT is 5 days, the decrease of nitrous accumulation rate is caused by the adaptation of sludge. So the NH+4-N sludge load of high concentration nitrous accumulation system should not be too high; 2)With the increase of DO, the ammonium degradation of high concentration nitrous nitrifying system is gradually increased. When the DO is less than 0.7 mg/L, it is not conductive to the ammonium degradation. When the DO is higher than 2 mg/L, the oxide of NH+4-N is changed into the increased of NO3-N and the nitrous accumulation rate declined. If the concentration of DO is controlled within 0.7to 1.3 mg/L, the high concentration nitrous nitrifying system can have a good NH+4-N degradation rate and nitrous accumulation rate.     

全自动换血治疗G-6-PD缺陷症并发急性溶血的疗效观察

目的 :观察全自动换血治疗危重G 6 PD缺陷症并发急性溶血的疗效。方法 :对 1 0例危重G 6 PD缺陷症并发急性溶血采用全自动换血治疗。结果 :总胆红素由换血前 (5 33.1 4± 1 1 5 .6 5 ) μmol/L下降至换血后 (2 95 .5 0± 88.5 1 )μmol/L ,换出率是 4 4 .5 7% ,血红蛋白增加 34.1 2 % ,红细胞增加 4 3.30 % ,换血过程无明显不良反应。结论 :全自动换血是抢救危重G 6 PD缺陷症并发急性溶血的简单、实用、安全、有效的方法     

A Lymphoid Organ Specific Anti-Lipopolysaccharide Factor from Litopenaeus vannamei Exhibits Strong Antimicrobial Activities

Different shrimp species are known to possess apparent distinct resistance to different pathogens in aquaculture. However, the molecular mechanism underlying this finding still remains unknown. One kind of important antimicrobial peptides, anti-lipopolysaccharide factors (ALF), exhibit broad-spectrum antimicrobial activities. Here, we reported a newly identified ALF from the shrimp Litopenaeus vannamei and compared the immune function with its counterpart in the shrimp Fenneropenaeus chinensis. The ALF, designated as LvALF8, was specifically expressed in the lymphoid organ of L. vannamei. The expression level of LvALF8 was apparently changed after white spot syndrome virus (WSSV) or Vibrio parahaemolyticus challenges. The synthetic LBD peptide of LvALF8 (LvALF8-LBD) showed strong antibacterial activities against most tested Gram-negative and Gram-positive bacteria. LvALF8-LBD could also inhibit the in vivo propagation of WSSV similar as FcALF8-LBD, the LBD of LvALF8 counterpart in F. chinensis. However, LvALF8-LBD and FcALF8-LBD exhibited apparently different antibacterial activity against V. parahaemolyticus, the main pathogen causing acute hepatopancreatic necrosis disease (AHPND) of affected shrimp. A structural analysis showed that the positive net charge and amphipathicity characteristics of LvALF8-LBD peptide were speculated as two important components for its enhanced antimicrobial activity compared to those of FcALF8-LBD. These new findings may not only provide some evidence to explain the distinct disease resistance among different shrimp species, but also lay out new research ground for the testing and development of LBD-originated antimicrobial peptides to control of shrimp diseases.     

苹果褪绿叶斑病毒的ELISA检测技术研究

比较了直接双抗体夹心法 ( DAS- ELISA)和间接双抗体夹心法 ( F( ab) 2 -ELISA)检测 CLSV的范围 (不同株系 ) ,并分析了在病毒提取介质中加入某些特殊成分后 ,对直接双抗体夹心法检测 CLSV效果的影响。结果表明 ,间接法可以检测较宽范围的病毒株系 ;当常规病毒提取介质中加入尼古丁、氯化镁和聚酰胺时 ,标准双抗体夹心法的检测范围也可以扩大     

北疆高产棉田群体光合速率及与产量关系的研究

研究了北疆２２５０ｋｇ·ｈｍ－２高产条件下棉花群体光合速率的变化及与产量之间的关系。结果表明，２２５０ｋｇ·ｈｍ－２高产棉田群体光合速率在整个生育期的变化呈单峰型曲线，在盛花期以前稳定上升，至盛铃期达最大，值为４．０５ｇ·ｍ－２·ｈ－１，吐絮盛期尚保持较高水平。全生育期平均值为２．６３ｇ·ｍ－２·ｈ－１。群体光合速率的日变化也呈单峰曲线，在１ｄ内至北京时间１４∶００～１５∶００达最大值。与其它产量条件下棉花群体光合速率的变化结果进行统计分析，盛花结铃期和吐絮期群体光合速率与皮棉产量呈显著正相关。在生产上，加强棉田后期管理，保持较高的群体光合速率，有利于棉花高产。     

兰新铁路沙泉子段风沙危害特点及防治

本文是在大量野外调查基础上,就兰新铁路沙泉子段风沙危害的方式、成因做了较为详尽的分析,并提出了机械防护与生物防护相结合的综合防护模式,为解决此区间沙害提供了依据。     

温度和规格对波纹巴非蛤耗氧率和排氨率的影响

本研究以波纹巴非蛤为实验材料,比较了温度和规格对其耗氧率和排氨率的影响。实验温度设为15、20、25和30℃,设立了大（A）和小（B）两种规格,其壳长分别为4.35 ± 0.19 cm 和3.53 ± 0.15 cm。结果表明温度对耗氧率和排氨率存在显著影响（P < 0.05）。规格对耗氧率存在显著影响（P > 0.05）,而对排氨率影响不显著（P > 0.05）。在温度15 - 30℃之间,波纹巴非蛤的耗氧率随温度升高而增大。在15和20℃条件下,B组的耗氧率显著大于A组（P < 0.05）;在25和30℃条件下,B组的耗氧率大于A组,但差异不显著（P > 0.05）。在温度15 - 30℃之间,波纹巴非蛤的排氨率随温度升高而增大。在各温度条件下,两组排氨率没有显著差异（P > 0.05）。     

京津冀都市圈农业合作战略研究

根据区域合作和一体化理论,比较长三角经济圈、珠三角经济圈、京津冀都市圈区域合作进程,研究京津冀都市圈合作的一般要求和农业合作的特点,比较北京农业和承德农业优劣势及农业资源情况,分析北京发展都市型现代农业为京津冀都市圈农业合作产生的动力,考察京承农业合作的现状以及对承德农业发展和北京环境保护、水资源涵养的成效,根据京承农业合作典型案例启示,结合区域农业合作实际和发展趋势,提出京津冀都市圈农业合作战略与合作框架协议的对策。