国内高架桥绿化及研究现状

从高架桥区的生态环境、适生种类的选择以及高架桥绿化的养护管理、景观配置模式四个方面对国内的高架桥绿化及研究现状进行了分析和探讨,指出了存在的问题和解决方法,并对高架桥绿化的前景进行了展望.     

左旋咪唑在生长育肥猪驱虫中的应用研究

本文探讨了5%左旋咪唑注射液按10mg/kg 体重剂量的不同驱虫次数对生长育肥猪驱虫效率、中毒反应及生产性能的影响。结果表明,1)在60～240日龄全期试验内,分别于60和150日龄各驱虫1次的试验Ⅰ组猪日增重极显著高出对照组(不驱虫)的23.9%(p<0.01);也显著高于只在60日龄驱虫1次的试验Ⅱ组(p<0.05),尽管Ⅱ组猪日增重显著高于对照组14.5%(p<0.05)。2)用药后,猪只应激毒性反应为100%,但均能自然康复。3)2次驱虫的效率比1次驱虫的高,且彻底。因此,用左旋咪唑给生长育肥猪驱虫,宜进行2次(分别于60和150日龄),可显著改善生长性能和提高经济效益,适宜在农(山)区养猪中推广。     

发展我国洪水保险的制度思考——美国洪水保险制度的借鉴与启示

洪水保险是一项重要的非工程防洪措施.美国是世界上最早建立国家强制性洪水保险体制的国家,经过艰难曲折的探索,闯出了一条成功之路.我国的洪水保险体制还处于积极探索阶段.中美两国尽管国情差异较大,但以洪水保险作为推进洪泛区洪水风险管理的经济手段,并以立法和国家计划等措施来保障实施的作法是值得借鉴的.本文就美国洪水保险制度的发展与沿革,寻找对我国建立洪水保险制度一些有益的启示,并就此提出了一些建设性意见.     

Cell proliferation and nestin expression in cortex and SEZ of MCAO rats

AIM:To explore the effect of brain ischemia injury on cell proliferation and nestin expression in cortex and subependymal zone (SEZ).METHODS:Using a local brain ischemia model(MCAO), BrdU positive cells of cortex and subependymal zone (SEZ), also nestin positive cells, were observed by immunohistochemistry.RESULTS:BrdU and nestin positive cells in SEZ of MCAO rats were obviously increased. In cortex, only nestin positive cells were observed.CONCLUSION:Neural stem cells in SEZ and cortex were activated after brain ischemia, it may be related with neural recovery after brain ischemia injury.     

The application of atomic force microscope in clinic diagnosis of erythrocytes

AIM: To investigate the application of atomic force microscope (AFM) in clinic diagnosis. METHODS: Topographic images and some parameters of large range field and microstructures of erythrocytes in the blood of normal subjects, lung cancer and myelodisplastic syndrome (MDS) patients were examined by atomic force microscope. RESULTS: Many clear topographic images of many erythrocytes, single erythrocyte, and microstructure of erythrocyte membrane surface were obtained. Many erythrocytes in lung cancer patients were found to change into echinocytes. One erythrocyte had 10-20 protuberances, most of which, with a mean width of 589. 0 nm and a length of 646. 7 nm, were on the edge of cells. The protuberances on the center of echinocytes are lodged and embedded. The erythrocytes of MDS patients were biconcave in shape. Many apertures with different diameters of tens to hundreds nanometer appeared on the surface of cell membrane. CONCLUSIONS: AFM can be widely applied in clinic pathological inspection, including quantification of cells, obtainment and comparison of many parameters (such as diameter, thickness, volume, surface, surface area/volume ratio), observation of topograph of single cell, and observation and comparison of membrane surface microstructure of cells, and so on.     

Selection and amplification of the liver stem cell subset from rat bone marrow cells with a medium containing cholestatic serum in vitro

AIM: To explore the feasibility of direct separat and selective enlargement of the bone marrow-derived liver stem cells (BDLSC) from bone marrow cells with a culture system containing cholestatic serum in vitro. METHODS: Bone marrow cells of rats were cultured with selective media containing 2%, 5%, 7% and 10% cholestatic rat serum, respectively. The BDLSC were then induced to proliferate with the addition of hepatocyte growth factor (HGF) on the firth day. BDLSC were characterized using immunocytochemistry and RT-PCR for lineage markers, glycogen staining and urea synthetic assay for functions 2 weeks later. RESULTS: Bone marrow cells were unble to form colony in the presence of 2% cholestatic serum and apopotosis appeared gradually in 7% or 10% cholestatic serum. The BDLSC survived in the medium containing 5% cholestatic serum while the other types of cells did not. The survival cells proliferated with a high speed during the second week and then formed hepatocyte-like colony-forming units (H-CFU). Cells in the H-CFU expressed the characteristic proteins of fetal hepatocytes. Furthermore, they had glycogen storage and urea synthesis functions, two of the critical features of hepatocytes. CONCLUSION: The selective micro-environment effectively selected BDLSC from the bone marrow cell, and will be a new way to provide an abundant source of donor hepatocytes for clinical cell therapy.     

入世后我国甜樱桃面临的机遇与挑战及发展对策

甜樱桃作为一种早熟、优质、保健果品,而且种植效益较高,在国内国际都正处于发展时期。根据世界甜樱桃生产和贸易情况,指出了我国在甜樱桃科学技术与生产上与世界先进甜樱桃生产国的差距,分析了我国加入WTO之后,我国甜樱桃面临的机遇和挑战,并且机遇大于挑战。如果有适当的科技投入,我国甜樱桃产业将可克服现在科技滞后的状态,发挥我国劳动力低廉和国土辽阔的优势,迅速扩大其生产总量,提高品质,扩大出口,增加农民的收入,2015年可望形成32.4～43.2亿元的社会总产值。     

大白菜细胞核雄性不育基因向自交系97A407的转育

根据复等位基因遗传假说 ,利用大白菜核不育系 9810 9和已知基因型的甲型两用系可育株 99135 2作为不育源 ,以大白菜自交系 97A40 7为目标亲本 ,经过 5个世代的转育 ,获得了具有 97A40 7遗传基因型的新甲型两用系、临时保持系和雄性不育系。     

花粉多糖注射剂质量标准中几个项目测定

根据中国药典对注射剂的要求,对花粉多糖的蛋白质、鞣质、树脂、草酸盐、炽灼残渣、钾离子、砷盐、重金属含量进行测定,均符合规定,达到质量标准。