Effect of membrane environment at high temperature on delayed rectifier K+ channel in hypothalamus neurons

AIM: To observe the effect of high temperature on the delayed rectifier K⁺ channel(I_K) in hypothalamus neurons METHODS: The kinetic properties of I K were measured by cell-attached mode of patche-clamp technique RESULTS: The conductance, long-term open constant(τ _o2) and open probability(Po) of I_K increased with increase of temperature (P <0.05), while more burst open and multi-order open were recorded Many types of currents occurred after heat stress at 43℃,and voltage-dependent property reduced and even depressed CONCLUSIONS: The activating action of I_K might be affected obviously by high temperature, the coupling between I K and body fever or heatstroke remained to be established.     

广西糯玉米品种种质构成及穗部性状选育探讨

对2011-2022年广西审定的173个糯玉米品种进行分析,研究广西糯玉米品种种质来源、构成,探析品种选育路径。结果表明,广西糯玉米主要种质由A (宜山糯)类、B (京科糯2000)类、C (万糯2000)类、D (燕禾金2000)类、E (中糯1号)类、F (中糯2号)类、G (衡白522)类、H (华珍)类、I (花甜糯179)类、J (NA群体)类、K (NB群体)类等11类种质构成,A、B类种质是广西糯玉米品种的基础种质。根据亲本主要种质类型,排除年份间的环境变化因素,可将93个品种归入AB等15个群组。各群组穗部性状相关分析表明,穗行数、穗粗与产量及其他穗部性状分别呈显著负效应及无显著效应,群组相关性受到种质组合及组配模式共同影响,通常情况下种质组合的影响力更强,涉及F类种质的群组则相反。因此,穗部性状选育重点要根据亲本主要种质类型确定。     

桐梓县杂交玉米氮磷钾的合理配施效应

采用"3414"平衡施肥方案,在桐梓县肥力较高的小黄沙泥土上进行了杂交玉米的肥料效应田间试验.结果表明,增产效果最佳的肥料配方为处理6,即尿素32.6kg·667m-2、普钙62.5kg·667m-2、氯化钾26.7kg·667m-2;从经济效益分析来看,以低氮低磷中钾处理的肥料增产效益最好,其肥料的产/投比为2.42,其肥料配方为处理12(N1P1K2),即尿素13.6kg·667m-2、普钙31.25kg·667m-2、氯化钾13.3kg·667-2.     

莲子草假隔链格孢的寄主范围及对空心莲子草的控制作用

 空心莲子草(Alteranthera philoxeroides)由于具有繁殖迅速、适应性强等特点,于20世纪50年代被引人我国江苏和浙江等地作为牲畜饲料植物栽培,随后相继传人全国各地并引起危害,成为我国大部分省区旱地和水域的恶性杂草之一。其微生物防治目前仍处于生物防治物的调查筛选阶段,已发现的病原真菌有假隔链格孢菌(Nimbya alternantherae)、炭疽菌(Colletotrichum gloeosporioides)、立枯丝核菌(Rhizoctonia solani)及链格孢(Alternaria sp.)等。     

灵芝孢子对肿瘤细胞生长的抑制效果研究

应用3种肿瘤细胞模型对未破壁灵芝孢子、机械破壁灵芝孢子和全灵芝剥壁孢子进行抗肿瘤试验研究。结果表明,未破壁及不同破壁方式的灵芝孢子对人恶性乳腺癌细胞(MT-1)、人恶性淋巴癌细胞株(Jurkat)、人恶性脑肿瘤细胞(U343)均有不同程度的抑制作用,抑制效果强弱为全灵芝剥壁孢子机械破壁灵芝孢子未破壁灵芝孢子,表明破壁的确能提高灵芝孢子对肿瘤细胞的抑制作用,而且选择破壁的方法也很重要,生物酶法破壁能使灵芝孢子的生物活性成分得到充分释放,所以大大提高灵芝孢子的功效。     

ASR蛋白与植物的抗逆性研究进展

 ASR蛋白（the abscisic acid,stress,ripening protein）是生物体中广泛存在的一类与渗透调节有关的家族蛋白,植物受到逆境胁迫（如干旱、低温、盐胁迫、ABA等）后,ASR蛋白大量表达,可以减轻逆境引起的伤害。ASR蛋白在细胞中可以稳定细胞膜结构,作为分子伴侣,具有结合离子和防止氧化等作用,被认为是在胁迫过程中对植物起保护作用的物质之一。针对这些重要特性,综述了ASR蛋白分类、结构和编码基因及其表达调控方式及ASR基因表达、ASR蛋白积累与植物抗逆性的关系等方面的研究进展。     

High level expression of recombinated hepatocyte growth factor in human umbilical cord mesenchymal stem cells

AIM: To construct a lentiviral vector encoding human hepatocyte growth factor (hHGF) for transfection,and to observe the expression of hHGF in human umbilical cord mesenchymal stem cells.METHODS: pUC-SRα/hHGF was subcloned into the expression vector pWPI to construct recombinant pWPI-hHGF.hHGF was identified by gene sequence.Recombinant lentivirus was produced by pWPI-hHGF,pAX2 and pMD2G altogether transient transfection into 293T cells using calcium phosphate method.The pWPI-hHGF and the contructed pWPI-GFP were transfected into human umbilical cord mesenchymal stem cells by the Lipofectamin 2000.Through counting by the fluorescent microscope,the efficiency of the transfection was identified.The expressions of hHGF and GFP in human umbilical cord mesenchymal stem cells were also detected.The concentration of hHGF in cell culture medium was determined by enzyme linked immunosorbent assay (ELISA).RESULTS: DNA sequence showed that hHGF cDNA was correctly inserted into pWPI vector.The positive rate of hHGF transfecting 293T cells was 100 %.Bright green fluorescence in the transfected cells was observed under the fluorescent microscope after 24 h transfection with lentiviral plasmid pWPI-hHGF-GFP,and the transfection rate reached 80%.The difference was distinct between the pWPI-hHGF group and control group in the secretive level of hHGF by Western blotting and the ELISA (P<0.01).CONCLUSION: The recombinant pWPI-hHGF plasmid was successfully constructed and efficient,stable and ectopic expression of hHGF was accomplished in human umbilical cord mesenchymal stem cells.     

贝加尔针茅＋线叶菊草地退化系列的定量划分和动态模型的建立

对贝加尔针茅(Stipa baicalensis) 线叶菊(Filifolium sibiricum)草地中贝加尔针茅、麻花头(Serratula centauroides)、日荫菅(Carex pediformis)和菊叶委陵菜(Potentilla tanacetifolia)4种主要植物的盖度、频度和植被指数进行了分析.结果表明: ①通过SPSS的聚类分析,实验区草地可以划分为3个草地退化系列,即轻度退化、中度退化、重度退化;②随草地退化程度的增强,贝加尔针茅的盖度、频度和指数表现出降低的趋势,麻花头与日荫菅的盖度、频度和植被指数表现出先增加后降低的趋势,而菊叶委陵菜盖度、频度和植被指数则表现出增加的趋势;③轻度退化、中度退化、重度退化3个不同退化系列的草地变化可通过 Y1=0.020 5X1 0.003 9X2 - 0.003 2X3 0.003 9X4 - 47.245 6;Y2=0.005 4X1 0.005 2X2-0.003 4X3 0.011 9X4-16.734 1;Y3=0.002 2X1 0.003 5X2-0.001 6X3 0.004 6X4-3.854 8(其中,Y表示最终的变化值,X1,X2,X3,X4分别表示草地最优状态时贝加尔针茅、麻花头、日荫菅和菊叶委陵菜4种植物的指数)来反映.     

间歇供水条件下点源入渗规律研究

对间歇供水和连续供水方式下的杨凌粘壤土点源入渗进行了室内对比研究。结果表明:间歇供水地表点源入渗水平和竖直方向的最大入渗距离与入渗时间存在显著的幂函数关系;在灌水定额相同的条件下,大滴头流量(4L/h)间歇滴灌与小滴头流量(2L/h)连续滴灌相比较,间歇供水能有效增加水平湿润距离,同时垂向入渗距离降低15%;对应于同一灌水量,采用30min、60min和120min等3种间歇周期对点源入渗的水平和垂向入渗距离无明显影响。     

果树中基因枪法遗传转化的研究进展

目前,应用于果树遗传转化的方法主要有农杆菌介导法和DNA直接导入法。介绍了DNA直接导入法中基因枪转化技术的原理、类型和基因枪的轰击参数、受体材料、基因型、轰击前后的培养条件等对其遗传转化频率的影响,并系统地概述了基因枪法在选择标记基因、报告基因、改良果树性状相关基因、启动子及多基因遗传转化中的应用研究进展,同时对果树基因枪转化研究中存在的问题及应用前景进行了讨论。