全文获取类型
收费全文 | 218235篇 |
免费 | 12413篇 |
国内免费 | 3339篇 |
专业分类
林业 | 10340篇 |
农学 | 9773篇 |
基础科学 | 3827篇 |
27277篇 | |
综合类 | 42851篇 |
农作物 | 8547篇 |
水产渔业 | 10125篇 |
畜牧兽医 | 103140篇 |
园艺 | 3245篇 |
植物保护 | 14862篇 |
出版年
2021年 | 2202篇 |
2020年 | 2271篇 |
2019年 | 2655篇 |
2018年 | 3124篇 |
2017年 | 3743篇 |
2016年 | 3470篇 |
2015年 | 3537篇 |
2014年 | 3874篇 |
2013年 | 8465篇 |
2012年 | 6978篇 |
2011年 | 8477篇 |
2010年 | 5968篇 |
2009年 | 5729篇 |
2008年 | 7840篇 |
2007年 | 7533篇 |
2006年 | 7118篇 |
2005年 | 6638篇 |
2004年 | 5872篇 |
2003年 | 5788篇 |
2002年 | 5578篇 |
2001年 | 6430篇 |
2000年 | 6334篇 |
1999年 | 5288篇 |
1998年 | 2497篇 |
1997年 | 2342篇 |
1996年 | 2204篇 |
1995年 | 2569篇 |
1994年 | 2291篇 |
1993年 | 2186篇 |
1992年 | 4201篇 |
1991年 | 4479篇 |
1990年 | 4451篇 |
1989年 | 4325篇 |
1988年 | 3956篇 |
1987年 | 4065篇 |
1986年 | 4144篇 |
1985年 | 3884篇 |
1984年 | 3320篇 |
1983年 | 2932篇 |
1982年 | 2008篇 |
1979年 | 2948篇 |
1978年 | 2419篇 |
1977年 | 2045篇 |
1975年 | 2020篇 |
1974年 | 2683篇 |
1973年 | 2694篇 |
1972年 | 2632篇 |
1971年 | 2410篇 |
1970年 | 2254篇 |
1969年 | 2101篇 |
排序方式: 共有10000条查询结果,搜索用时 500 毫秒
1.
为揭示睡莲(基部被子植物)的开花特性,探究雄蕊在睡莲花瓣节律性开放过程中的作用.本研究以蓝鸟睡莲(Nymphaea 'Blue Bird')为实验材料,在去雄(摘除雄蕊)后记录花瓣开放角度和萎蔫时间,并于去雄后第6、24、48及72小时测定花瓣的生理生化指标变化.结果 显示,去雄后睡莲花瓣的开放角度小于对照组,在第48小时与对照组开放角度差异最大,达到49.31°,而去雄后花瓣萎蔫的时间与对照组相同,均出现在第72小时;去雄引起了花瓣多项生理生化指标发生变化,其中,含水量在第24小时与72小时显著低于对照组,比对照组分别低0.7%与0.9%;可溶性糖含量变化显著,在第24小时和48小时比对照组分别高出0.48与0.68 mg/g;脯氨酸(Pro)含量在第72小时显著高于对照组;过氧化物酶(POD)活性从第24小时起比对照组显著升高;而丙二醛(MDA)含量则与对照组无明显差异.以上结果说明,去雄导致了睡莲花瓣开放角度及生理状态发生改变,该发现进一步地揭示了睡莲的开花特性,首次证明了雄蕊对于花瓣节律性开放具有调节作用,为花瓣开放闭合及切花保鲜等领域的研究提供了新的思路. 相似文献
2.
旨在调查川西北牦牛哺乳动物正呼肠孤病毒(MRV)的感染情况并分离病毒。采用RT-PCR方法,对采自川西北15个牧场的72份牦牛腹泻粪便样本和其中5个牧场的15份腹泻牦牛血清样本进行MRV检测,阳性样本进一步用分型PCR确定其血清型。结果显示,粪便样本中MRV检出率为20.83%(15/72),血清2型的比例为60%(9/15);血清样本中MRV检出率为40%(6/15),血清2型的比例为83.33%(5/6);未检测到其他血清型。成功地从腹泻粪便中分离到1株MRV血清2型毒株(TCID50为4×10-8.56·mL-1),并获得长度为23 587 bp的分离株全基因组,该分离株与中国猪源毒株的遗传关系最近;与GenBank中所有的MRV S1基因相比,该分离株有4个独特的氨基酸突变。本研究从牦牛中检测到MRV,并分离到1株牛源MRV血清2型毒株,为进一步研究MRV血清2型生物学特性奠定了基础。 相似文献
3.
Shelley J. Newman Stephen A. Smith Kurt Zimmerman 《Journal of veterinary diagnostic investigation》2021,33(3):566
A large, firm, multi-cystic mammary gland mass grew slowly over 4 y in a 12-y-old, female Finn–Shetland cross sheep. A diagnosis of epithelial malignancy was suspected following fine-needle aspiration cytology at 30 mo after initial observation. The sheep was euthanized when the flock was downsized 18 mo later. A field postmortem examination revealed a large mammary mass, but an absence of metastases to internal organs. Imprint cytology of the mammary tissue supported a benign proliferative process. Histologically, mammary tissue was obliterated by cystic, tubular, and papillary adenomatous arrangements of mammary epithelium, with an anaplastic component, consistent with mammary carcinoma arising in an adenoma. IHC showed strong nuclear positivity to the antibody against progesterone receptor and minimal positivity to the antibody against estrogen receptor alpha expression. Intrinsic subtyping for basal or luminal epithelial origin was attempted through adaptation of companion animal IHC classification panels; high- and low-molecular-weight cytokeratins (CK5, CK8, CK18) failed to stain, but p63 expression for basal epithelium was positive. 相似文献
4.
5.
Impact of grassland contract policy on soil organic carbon losses from alpine grassland on the Qinghai–Tibetan Plateau 下载免费PDF全文
J. Cao Y. Gong E. T. Yeh N. M. Holden J. F. Adamowski R. C. Deo M. Liu J. Zhou J. Zhang W. Zhang S. Zhang D. Sheng S. Yang X. Xu M. Li Q. Feng 《Soil Use and Management》2017,33(4):663-671
Carbon storage in the soils on the Qinghai–Tibetan Plateau plays a very important role in the global carbon budget. In the 1990s, a policy of contracting collective grasslands to smaller units was implemented, resulting in a change from the traditional collective grassland management to two new management patterns: a multi‐household management pattern (MMP: grassland shared by several households without enclosures) and a single‐household management pattern (SMP: grassland enclosed and used by only one household). In 2016, 50 MMP and 54 SMP winter pastures on the Qinghai–Tibetan Plateau were sampled to assess the differences in soil organic carbon (SOC) between the two management patterns. Results showed that average SOC was significantly greater under MMP than under SMP, with an estimated 0.41 Mg C/ha/yr lost due to SMP following the new grassland contract. Based on the government's grassland policy, four grassland utilization scenarios were developed for both summer and winter pastures. We found that if the grassland were managed under SMP, likely C losses ranged between 0.31 × 107 and 6.15 × 107 Mg C/yr across the Qinghai–Tibetan Plateau relative to MMP, which more closely resembles pre‐1990s grassland management. Previous estimates of C losses have only considered land use change (with cover change) and ignored the impacts driven by land management pattern changes (without cover change). The new data suggest that C losses from the Qinghai–Tibetan Plateau are greater than previously estimated, and therefore that the grassland contract policy should be reviewed and SMP households should be encouraged to reunite into the MMP. These findings have potential implications for land management strategies not only on the Qinghai–Tibetan Plateau but also other grazing regions globally where such practices may exist. 相似文献
6.
Utilizing a DUALEM‐421 and inversion modelling to map baseline soil salinity along toposequences in the Hunter Valley Wine district 下载免费PDF全文
In the oldest commercial wine district of Australia, the Hunter Valley, there is the threat of soil salinization because marine sediments underlie the area. To understand the risk requires information about the spatial distribution of soil properties. Electromagnetic (EM) induction instruments have been used to identify and map the spatial variation of average soil salinity to a certain depth. However, soils vary with depth dependent on soil forming factors. We collected data from a single‐frequency and multiple‐coil DUALEM‐421 along a toposequence. We inverted this data using EM4Soil software and evaluated the resultant 2‐dimensional model of true electrical conductivity (σ – mS/m) with depth against electrical conductivity of saturated soil pastes (ECp – dS/m). Using a fitted linear regression (LR) model calibration approach and by varying the forward model (cumulative function‐CF and full solution‐FS), inversion algorithm (S1 and S2), damping factor (λ) and number of arrays, we determined a suitable electromagnetic conductivity image (EMCI), which was optimal (R2 = 0.82) when using the full solution, S2, λ = 3.6 and all six coil arrays. We conducted an uncertainty analysis of the LR model used to estimate the electrical conductivity of the saturated soil‐paste extract (ECe – dS/m). Our interpretation based on estimates of ECe suggests the approach can identify differences in salinity, how these vary with parent material and how topography influences salt distribution. The results provide information leading to insights into how soil forming factors and agricultural practices influence salinity down a toposequence and how this can guide soil management practices. 相似文献
7.
8.
Emily Corey Tommi Linnansaari Stephen J. Dugdale Normand Bergeron Jean-François Gendron Michel Lapointe Richard A. Cunjak 《Ecology of Freshwater Fish》2020,29(1):50-62
Climate change is expected to increase the frequency and magnitude of extreme thermal events in rivers. The Little Southwest Miramichi River (LSWM) and the Ouelle River (OR) are two Atlantic salmon (Salmo salar) rivers located in eastern Canada, where in recent years, water temperatures have exceeded known thermal limits (~23°C). Once temperature surpasses this threshold, juvenile salmon exploit thermal heterogeneity to behaviourally thermoregulate, forming aggregations in coolwater refuges. This study aimed to determine whether the behavioural thermoregulation response is universal across rivers, arising from common thermal cues. We detailed the temperature and discharge patterns of two geographically distinct rivers from 2010 to 2012 and compared these with aggregation onset temperature. PIT telemetry and snorkelling were used to confirm the presence of aggregations. Mean daily maximum temperature in 2010 was significantly greater in the OR versus the LSWM (p = 0.005), but not in other years (p = 0.090–0.353). Aggregations occurred on 14 and 9 occasions in the OR and LSWM respectively. Temperature at onset of aggregation was significantly greater in the OR (Tonset = 28.3°C) than in the LSWM (Tonset = 27.3°C; p = 0.049). Logistic regression models varied by river and were able to predict the probability of aggregation based on the preceding number of hours >23°C (R2 = 0.61 & 0.65; P50 = 27.4°C & 28.9°C; in the OR and LSWM respectively). These results imply the preceding local thermal regime may influence behaviour and indicate a degree of phenotypic plasticity, illustrating a need for localised management strategies. 相似文献
9.
Karen E. Jensen J. Christopher Taylor Robert J. Barry Louis R. D'Abramo D. Allen Davis Stephen A. Watts 《Journal of the World Aquaculture Society》2019,50(3):614-621
Sea urchins produce high‐energy, membrane‐bound fecal pellets that contain residual nutrients and large quantities of microbiota. These egesta are readily consumed by the shrimp, Litopenaeus vannamei. Egesta of the sea urchin, Lytechinus variegatus, were evaluated as a feed supplement or total replacement for a commercial shrimp diet. Shrimp were stocked at 0.49 g ± 0.06 g initial body weight and housed individually in 2.8‐L tanks in a commercial recirculating zebrafish system. Shrimp were assigned to one of six diets: commercial shrimp feed, reference sea urchin feed, collected dried sea urchin egesta, collected wet sea urchin egesta, half ration of shrimp feed and half collected wet sea urchin egesta, and egesta naturally produced by two sea urchins in polyculture. Equivalent dry matter amounts of each diet were proffered to shrimp in each treatment twice daily, except for those that had complete access to natural egesta excreted by sea urchins in polyculture. Sea urchins were proffered a reference sea urchin feed at 2% body weight daily. After 27 days, shrimp proffered collected dried or wet egesta did not differ significantly in percent weight gain and showed the lowest weight gain. The percent weight gain of shrimp fed the commercial shrimp diet did not differ significantly from that of the shrimp fed half commercial shrimp diet and half egesta. The highest weight gain was recorded for those shrimp that consumed the untouched egesta produced by sea urchins in polyculture. These data suggest that consumed egesta have noteworthy nutritional value and therefore would be beneficial to the culture of extractive species in an integrated multitrophic aquaculture system. 相似文献
10.
Still Only One Earth. Progress in the 40 years since the First UN Conference on the Environment. By R.E. Hester & R.M. Harrison (eds). Published by Royal Society of Chemistry,Cambridge, 2015. vii + 285 pp. £67.50. Hardback (ISBN 978‐1‐78262‐076‐1).
下载免费PDF全文
R. L. Hough 《Soil Use and Management》2017,33(1):176-176