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排序方式: 共有88条查询结果,搜索用时 15 毫秒
1.
Acetolactate synthase (ALS) is the target enzyme for four distinct families of compounds: sulfonylureas (SUs), imidazolinones, triazolopyrimidine sulfonanilides, and pyrimidinyl oxybenzoates. We cloned and sequenced the fragments encoding ALS genes from biotypes of Monochoria vaginalis susceptible (S) and resistant (R) to SU-herbicides. The nucleotide sequences of the 39 bp Domain A region for R M. vaginalis biotype differed from that of the S biotype by a single nucleotide substitution at variable Pro codon of Domain A (CCT to TCT), predicting a Pro in the S but a Ser in the R biotype. No nucleotide differences between S and R M. vaginalis were observed in Domain D. We suggest that the amino acid substitution at Domain A region is responsible for resistance to SU-herbicides in M. vaginalis collected from Ushiku City, Ibaraki Prefecture, Japan. 相似文献
2.
Hiroko Yamaguchi Misako Nakaya Gen Kaneko Chie Yoneda Toshitaka Mochizuki Katsuya Fukami Hideki Ushio Shugo Watabe 《Fisheries Science》2013,79(2):327-334
The taste and extractive components of boiled dorsal muscle and broth prepared from half-smooth golden puffer Lagocephalus spadiceus caught in Japan and from those imported from China were compared. In the sensory test, the first taste, elasticity, and saltiness of boiled muscle from Japanese (domestic) fish were higher than those of the imported fish, whereas the orthonasal fishy smell of the imported fish was higher than that of the domestic fish. The first taste, aftertaste, retronasal fishy smell, sweetness, saltiness, and umami of the broth prepared from dorsal muscle of the domestic fish were higher than those of the imported fish, whereas orthonasal fishy smell and bitterness of the imported fish were higher than those of the domestic fish. Most panelists preferred the overall taste of the domestic fish to that of the imported fish. The concentration of trimethylamine in the trichloroacetic acid extracts from boiled muscle and broth of the imported fish was higher than that of the domestic fish, suggesting that this substance contributes to the orthonasal fishy smell of the imported fish. 相似文献
3.
An attempt was made to account for quantitatively measured herbicidal performance of foliage-applied 5-hydroxy-3-methyl-2–oxo-imidazolidine-1-carboxamide derivatives by their photosynthesis-inhibiting activity and systemicity. Photosynthesis-inhibiting activity was estimated from the increase of chlorophyll fluorescence intensity in Chlorella vulgaris Beijer cells measured by a microplate scanner, and systemicity was also evaluated by computer-aided chlorophyll fluorescence imaging. The highest herbicidal performance was recorded for N-cyclohexyl-5-hydroxy-3-methyl-2-oxo-imidazo~idine-l-carboxamide, a compound with the second strongest photosynthesis-inhibiting activity and intermediate systemicity. Though neither photosynthesis-inhibiting activity nor systemicity showed significant correlation with the actual herbicidal performance in simple regression analyses, a high predictability was found for a multiple regression on both parameters as two independent variables, suggesting that these two factors work cooperatively in the field performance. 相似文献
4.
Umehashi M Imamura T Akiyama S Kimachi K Tokiyoshi S Mikami T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(11):1017-1021
In order to confirm the in vivo effectiveness of anti- feline herpesvirus type 1 (FHV-1) mouse-cat chimeric antibody (FJH2), and anti-feline calicivirus (FCV) mouse-cat chimeric antibody (F1D7), cats that had been experimentally infected with FHV-1 or FCV were administered intravenously with the chimeric antibodies, and observed for clinical manifestations. The symptoms due to FHV-1 or FCV infection in the cats administered FJH2 or F1D7 were obviously decreased when compared with those of the non-administered control cats. From these results, it was confirmed that both FJH2 and F1D7 were effective at reducing the appearance of symptoms due to FHV-1 and FCV infection, respectively. 相似文献
5.
6.
Effect of shrimp head protein hydrolysates on the state of water and denaturation of fish myofibrils during dehydration 总被引:1,自引:0,他引:1
Yaowalux?Ruttanapornvareesakul Misako?Ikeda Kenji?HaraEmail author Kazufumi?Osako Orawan?Kongpun Yukinori?Nozaki 《Fisheries Science》2005,71(1):220-228
ABSTRACT: To utilize fisheries waste products as food materials with functional properties, shrimp head protein hydrolysates (SHPH) from three species of shrimp, that is, Northern pink shrimp ( Pandalus eous ), Endeavour shrimp ( Metapenaeus endeavouri ) and Black tiger shrimp ( Penaeus monodon ), were produced by enzymatic hydrolysis using endopeptidase derived from Bacillus subtilis and exopeptidase derived from Aspergillus oryzae at a level of 0.1% (w/w). SHPH were rich in protein (90–91%) and amino acids (71–84%) but little fat (0.01–0.02%). The average molecular weight of SHPH was 300–1400. The effect of 5% SHPH (dry basis) addition on the state of water and denaturation of lizard fish myofibrils (Mf) during the dehydration process was evaluated by the desorption isotherm and the Ca-ATPase activity, and compared with the effect of sodium glutamate (Na-Glu). SHPH decreased the water activity and the Ca-ATPase inactivation, and increased monolayer sorbed water and multilayer sorbed water of Mf, although these effects of SHPH were smaller than those of Na-Glu. These findings suggest that the SHPH suppressed dehydration-induced denaturation of myofibrillar protein by stabilizing the hydrated water surrounding myofibrils. 相似文献
7.
8.
Effects of isoprothiolane and phytosterol on adipocyte metabolism and fatty acid composition of serum and tissue lipids in rats. 总被引:2,自引:0,他引:2
H Katamoto N Yoneda Y Shimada 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(5):905-910
Isoprothiolane at a dose of 250 mg/kg or phytosterol 50 mg/kg was orally administered to rats once a day for 2 weeks. Basal [U-14C]glucose conversion rate to total lipids in isolated adipocytes of the rats was significantly decreased by treatment with isoprothiolane (54%) or phytosterol (82%). Adipocytes from the rats with isoprothiolane released less glycerol than those from control rats only at an epinephrine concentration of 10 microM. The serum level of total cholesterol was depressed by phytosterol ingestion. The level of non-esterified fatty acids (NEFA) was increased by isoprothiolane. Desaturation in fatty acid composition of phospholipid or cholesterol ester was observed in serum, liver and adipose tissue of the rats treated with either drug. These results suggest that either drug may have common effects by preventing lipid deposition into adipocytes and accelerating fatty acid desaturation in tissue lipids. 相似文献
9.
10.
Expression and purification of recombinant swine interleukin-4 总被引:4,自引:0,他引:4
Nuntaprasert A Mori Y Fujita K Yoneda M Miura R Tsukiyama-Kohara K Kai C 《Comparative immunology, microbiology and infectious diseases》2005,28(1):17-35
The swine interleukin-4 (SwIL-4) cDNA was cloned by RT-PCR. It was expressed using an expression vector pQE30 in E. coli, a baculovirus AcNPV vector pVL1392 in insect cells, and a pCAGGS vector in mammalian cells. The rSwIL-4 proteins expressed from bacteria and insect cells were purified using a chelating affinity column and a mAb-coupled immunoaffinity column. The amount of the products and their bioactivities were compared. All recombinant cytokines were efficiently reacted with the specific antibodies and the molecular weight of rSwIL-4 was approximately 16 kDa in E. coli, 15 and 18 kDa in insect cells, and 15 and 20 kDa in mammalian cells. Variations of molecular weight observed in insect and mammalian cells were probably due to different modification ways of glycosylation. All these recombinant proteins retained their antigenicity and were biologically active in inducing human TF-1 cell proliferation in vitro. The simple purification method will make it possible to evaluate the in vitro and in vivo effects of IL-4 in pigs. 相似文献