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Phenotypic and functional analysis of bovine peripheral blood dendritic cells before parturition by a novel purification method 下载免费PDF全文
Tao Zhuang Megumi Urakawa Hidetoshi Sato Yuko Sato Teruaki Taguchi Tsuyoshi Umino Shiro Katto Koutaro Tanaka Kozue Yoshimura Naokazu Takada Hiroko Kobayashi Megumi Ito Michael T. Rose Yoshio Kiku Yuya Nagasawa Haruki Kitazawa Kouichi Watanabe Tomonori Nochi Tomohito Hayashi Hisashi Aso 《Animal Science Journal》2018,89(7):1011-1019
Dendritic cells (DCs) are specialized antigen presenting cells specializing in antigen uptake and processing, and play an important role in the innate and adaptive immune response. A subset of bovine peripheral blood DCs was identified as CD172a+/CD11c+/MHC (major histocompatibility complex) class II+ cells. Although DCs are identified at 0.1%–0.7% of peripheral blood mononuclear cells (PBMC), the phenotype and function of DCs remain poorly understood with regard to maintaining tolerance during the pregnancy. All cattle used in this study were 1 month before parturition. We have established a novel method for the purification of DCs from PBMC using magnetic‐activated cell sorting, and purified the CD172a+/CD11c+ DCs, with high expression of MHC class II and CD40, at 84.8% purity. There were individual differences in the expressions of CD205 and co‐stimulatory molecules CD80 and CD86 on DCs. There were positive correlations between expression of cytokine and co‐stimulatory molecules in DCs, and the DCs maintained their immune tolerance, evidenced by their low expressions of the co‐stimulatory molecules and cytokine production. These results suggest that before parturition a half of DCs may be immature and tend to maintain tolerance based on the low cytokine production, and the other DCs with high co‐stimulatory molecules may already have the ability of modulating the T‐cell linage. 相似文献
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Yamazaki H Wewengkang DS Nishikawa T Rotinsulu H Mangindaan RE Namikoshi M 《Marine drugs》2012,10(2):349-357
Two new tryptamine-derived alkaloids, named as leptoclinidamide (1) and (-)-leptoclinidamine B (2), were isolated from an Indonesian ascidian Leptoclinides dubius together with C2-α-D-mannosylpyranosyl-L-tryptophan (3). The structure of 1 was assigned on the basis of spectroscopic data for 1 and its N-acetyl derivative (4). Compound 1 was an amide of tryptamine with two β-alanine units. Although the planar structure of 2 is identical to that of the known compound (+)-leptoclinidamine B (5), compound 2 was determined to be the enantiomer of 5 based on amino acid analysis using HPLC methods. Compounds 1 to 4 were evaluated for cytotoxicity against two human cancer cell lines, HCT-15 (colon) and Jurkat (T-cell lymphoma) cells, but none of the compounds showed activity. 相似文献
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Tomomi Kaku Kei’ichi Baba Toru Taniguchi Manabu Kurita Ken-ichi Konagaya Katsuaki Ishii Teiji Kondo Satoshi Serada Haruka Iizuka Rumi Kaida Teruaki Taji Yoichi Sakata Takahisa Hayashi 《Journal of Wood Science》2012,58(4):281-289
The transgenic expression of Aspergillus xyloglucanase cDNA (AaXEG2) with 35S promoter in the leaves of open field-grown poplars was studied. The level of xyloglucan in the transgenic poplars was decreased to 15–16% in the non-fertile soil (forest-field soil) and to 21–22% in the fertile soil (farming-field soil) compared with that of the wild-type poplars. The leaves exhibited a smaller surface area with more rounded teeth than those of the wild-type plants, similar to the sun leaf variety that was grown in the incubation room and subsequently greenhoused. The majority of total veins with water-conducting vascular bundles were shorter in the leaves of the transgenic poplars than those of the wild type. This decrease in vein length may result from a decrease in xyloglucan during leaf development, from which large numbers of proteins were markedly downregulated in the leaves of the transgenic plants via proteomic analysis. It seems likely that the leaves of the transgenic poplars came to relax the edges of their tooth rather than extend their veins as a result of the loosening of the xyloglucan cellulose networks in the leaves. 相似文献
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Masaki TAKASU Nana HIRAMATSU Teruaki TOZAKI Hironaga KAKOI Telhisa HASEGAWA Masami MAEDA Huricha Satoshi KUSUDA Osamu DOI Tetsuma MURASE Harutaka MUKOYAMA 《Journal of Equine Science》2011,22(4):67-72
The objective of this study was to clarify the current status of endangered Kiso horse,
population statistics and biological traits, in order to take a step for the conservation
by scientific approach. We surveyed 125 Kiso horses (86.2% of the whole breed), analyzed
the construction of the population, and calculated the coefficient of inbreeding and
effective population size. Moreover, we confirmed coat color variations and the
traditional traits of the Kiso horse, and measured their height at the withers and chest
circumference to clarify their physical characteristics. The population pyramid of the
horses was stationary or contractive, suggesting a reduction of the population in the near
future. The effective population size of the horse (47.9) suggested that the diversity was
much less than their census size, and the high coefficient of inbreeding, 0.11 ± 0.07 on
average, suggested that the horses were surely inbred. The horses had only 4 coat colors;
bay, dark bay, buckskin dun, and chestnut, and 116 horses (92.8%) were bayish color,
suggesting the fixation in their coat color. Moreover, the majority of them had dorsal
stripe (83 horses; 66.4%), and the average heights at withers(131.9 ± 4.4 cm) and chest
circumference (167.1 ± 10.1 cm) were not significantly different between males and
females. 相似文献
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Analysis of the natural abundance 14C content of dibutyl phthalate (DBP) from two edible brown algae, Undaria pinnatifida and Laminaria japonica, and a green alga, Ulva sp., revealed that the DBP was naturally produced. The natural abundance 14C content of di-(2-ethylhexyl) phthalate (DEHP) obtained from the same algae was about 50–80% of the standard sample and the 14C content of the petrochemical (industrial) products of DBP and DEHP were below the detection limit. 相似文献
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Tetsuo Tamura Kazunori Inoue Hideto Nishiki Masafumi Sakata Makoto Seki Teruaki Koga Yoshihiro Ookubo Kazuhiro Akutsu Say Sato Kouichi Saitou Hikari Shinohara Terumi Kuraisi Hiroshi Kajikawa Mitsunori Kurihara 《Animal Science Journal》2019,90(1):62-70
We evaluated the influence on milk production of feeding early lactation cows a diet that included 14.5% crude protein (CP) and that did not meet methionine (Met) requirements or that met them by supplying rumen‐protected Met (RPMet). Thirty‐nine multiparous Holstein cows were allocated into two groups. For 15 weeks after calving, each group was fed one of the two total mixed rations, Control (n = 20) or Treatment (n = 19). The Treatment group received added RPMet at 0.034% (8 g/day) of the Control diet on dry matter basis. The adequacies of Met for the Control and Treatment groups were 96% and 106%, respectively, and for other amino acids, >110%. The CP level (14.5%) was 1 percentage point lower than that recommended by the Japanese Feeding Standard (2006). No between‐group differences were found in milk yield (40 kg/day), milk composition, plasma profile, rumen fermentation, nitrogen balance, or cow health. Met intake and the amount of rumen‐undegradable feed Met were higher in the Treatment group (p < 0.05). Microbial Met and total metabolizable Met did not differ between groups. Supplying RPMet in a 14.5% CP diet during early lactation did not dramatically affect milk production, because the amount of total metabolizable Met was unchanged. 相似文献
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Hitoshi Hatai Takashi Hatazoe Haruka Seo Teruaki Tozaki Shingo Ishikawa Noriaki Miyoshi Kazuhiro Misumi Seiji Hobo 《Journal of veterinary diagnostic investigation》2021,33(2):379
A 27-y-old Anglo-Arabian gelding with bay coat color was presented with a swelling of the left maxillary region. Fenestration on the left maxilla revealed that the left maxillary sinus was filled with black-red tissue. A portion of the tissue was excised and diagnosed histologically as malignant melanoma. Genotyping of the STX17 gene for gray coat color revealed that the horse did not have the “gray” factor. The horse was euthanized ~3 mo after first presentation. During autopsy, a black-to-gray mass extended from the left nasal cavity to the surrounding paranasal sinus and invaded the hard palate, cribriform plate, and the cranial portion of the left olfactory bulb. Moreover, identical black nodules were present in lymph nodes from the mandible to the larynx, and in the spleen, liver, kidney, and adrenal glands. However, masses were not found in the skin, perineal region, or pelvic cavity. All of the black-to-gray nodules were malignant melanomas that were histologically identical to the initial biopsy; tumor emboli were also found in the kidney. Sinonasal mucosal melanoma is a rare disease in horses. 相似文献